Gene description for Pfkp
Gene name phosphofructokinase, platelet
Gene symbol Pfkp
Other names/aliases 1200015H23Rik
9330125N24Rik
ATP-PFK
PFK-C
PFK-P
Species Mus musculus
 Database cross references - Pfkp
ExoCarta ExoCarta_56421
Vesiclepedia VP_56421
Entrez Gene 56421
UniProt Q9WUA3  
 Pfkp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pfkp
Molecular Function
    6-phosphofructokinase activity GO:0003872 IBA
    6-phosphofructokinase activity GO:0003872 ISO
    6-phosphofructokinase activity GO:0003872 ISS
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
    metal ion binding GO:0046872 IEA
    fructose-6-phosphate binding GO:0070095 IBA
    fructose-6-phosphate binding GO:0070095 IEA
    fructose-6-phosphate binding GO:0070095 ISO
Biological Process
    fructose 6-phosphate metabolic process GO:0006002 IBA
    fructose 6-phosphate metabolic process GO:0006002 IEA
    fructose 6-phosphate metabolic process GO:0006002 ISO
    glycolytic process GO:0006096 ISO
    fructose 1,6-bisphosphate metabolic process GO:0030388 IBA
    fructose 1,6-bisphosphate metabolic process GO:0030388 IEA
    fructose 1,6-bisphosphate metabolic process GO:0030388 ISO
    glycolytic process through fructose-6-phosphate GO:0061615 ISS
    canonical glycolysis GO:0061621 IBA
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    6-phosphofructokinase complex GO:0005945 IBA
    6-phosphofructokinase complex GO:0005945 ISS
    membrane GO:0016020 IBA
 Experiment description of studies that identified Pfkp in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pfkp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tia1  
Affinity Capture-MS Mus musculus
2 HTT 3064
Affinity Capture-MS Homo sapiens
3 Atxn1  
Proximity Label-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Mapt  
Affinity Capture-MS Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 Nanog  
Affinity Capture-MS Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
9 Ywhaz 22631
Affinity Capture-MS Mus musculus
10 Nedd4l 83814
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Pfkp is involved
PathwayEvidenceSource
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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