Gene description for CDC42SE2
Gene name CDC42 small effector 2
Gene symbol CDC42SE2
Other names/aliases SPEC2
Species Homo sapiens
 Database cross references - CDC42SE2
ExoCarta ExoCarta_56990
Vesiclepedia VP_56990
Entrez Gene 56990
HGNC 18547
UniProt Q9NRR3  
 CDC42SE2 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Prostate cancer cells 25844599    
 Gene ontology annotations for CDC42SE2
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IEA
    signaling adaptor activity GO:0035591 IDA
Biological Process
    phagocytosis GO:0006909 IEA
    regulation of cell shape GO:0008360 IEA
    regulation of signal transduction GO:0009966 IDA
    regulation of Rho protein signal transduction GO:0035023 IEA
Subcellular Localization
    phagocytic cup GO:0001891 IEA
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell projection GO:0042995 IEA
 Experiment description of studies that identified CDC42SE2 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for CDC42SE2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBB 203068
Affinity Capture-MS Homo sapiens
2 CDC42 998
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 VSIG4  
Affinity Capture-MS Homo sapiens
4 AURKB 9212
Affinity Capture-MS Homo sapiens
5 TUBB2B 347733
Affinity Capture-MS Homo sapiens
6 SSC5D 284297
Affinity Capture-MS Homo sapiens
7 RAC1 5879
Two-hybrid Homo sapiens
8 TMPRSS5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TUBB4B 10383
Affinity Capture-MS Homo sapiens
10 TSC22D3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
12 MYO19  
Affinity Capture-MS Homo sapiens
13 HSD11B1 3290
Affinity Capture-MS Homo sapiens
14 LIPH 200879
Affinity Capture-MS Homo sapiens
15 MB21D1  
Proximity Label-MS Homo sapiens
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