Gene description for RALGAPB
Gene name Ral GTPase activating protein, beta subunit (non-catalytic)
Gene symbol RALGAPB
Other names/aliases KIAA1219
RalGAPbeta
Species Homo sapiens
 Database cross references - RALGAPB
ExoCarta ExoCarta_57148
Vesiclepedia VP_57148
Entrez Gene 57148
HGNC 29221
UniProt Q86X10  
 RALGAPB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RALGAPB
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 ISS
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IPI
    protein heterodimerization activity GO:0046982 ISS
Biological Process
    Ral protein signal transduction GO:0032484 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 IEA
    activation of GTPase activity GO:0090630 ISS
 Experiment description of studies that identified RALGAPB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RALGAPB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PARP3 10039
Affinity Capture-MS Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LINC01587  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 IRGM 345611
Affinity Capture-MS Homo sapiens
6 SLMAP 7871
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 DLG3 1741
Affinity Capture-MS Homo sapiens
9 PFN1 5216
Proximity Label-MS Homo sapiens
10 RAB11A 8766
Proximity Label-MS Homo sapiens
11 LAMP1 3916
Proximity Label-MS Homo sapiens
12 GFOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 YWHAZ 7534
Affinity Capture-MS Homo sapiens
14 LAMP2 3920
Proximity Label-MS Homo sapiens
15 TMEM74  
Affinity Capture-MS Homo sapiens
16 MFAP5  
Affinity Capture-MS Homo sapiens
17 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RAB4A 5867
Proximity Label-MS Homo sapiens
19 ACACA 31
Negative Genetic Homo sapiens
20 DNAJC5 80331
Proximity Label-MS Homo sapiens
21 EPHA2 1969
Proximity Label-MS Homo sapiens
22 C12orf74  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DND1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 YWHAE 7531
Affinity Capture-MS Homo sapiens
25 DPH7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NTRK1 4914
Affinity Capture-MS Homo sapiens
27 RAB9A 9367
Proximity Label-MS Homo sapiens
28 SERPINB2 5055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FBXW7  
Affinity Capture-MS Homo sapiens
30 MEA1  
Affinity Capture-MS Homo sapiens
31 SGIP1  
Affinity Capture-MS Homo sapiens
32 RHOB 388
Proximity Label-MS Homo sapiens
33 NKIRAS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 STX6 10228
Proximity Label-MS Homo sapiens
35 PTPRH 5794
Proximity Label-MS Homo sapiens
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