Experiment description of studies that identified SHROOM3 in sEVs

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for SHROOM3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YWHAH 7533 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 YWHAE 7531 Affinity Capture-MS Homo sapiens 3 SFN 2810 Affinity Capture-MS Homo sapiens 4 Gspt1 14852 Affinity Capture-MS Mus musculus 5 FBXO45 200933 Affinity Capture-MS Homo sapiens 6 RPS6KA2   Affinity Capture-MS Homo sapiens 7 DNAJB14   Proximity Label-MS Homo sapiens 8 MYCN   Affinity Capture-MS Homo sapiens 9 DNAJC11 55735 Proximity Label-MS Homo sapiens 10 OBSL1 23363 Affinity Capture-MS Homo sapiens 11 Psmb5 19173 Affinity Capture-MS Mus musculus 12 KIF14 9928 Affinity Capture-MS Homo sapiens 13 KIF20A 10112 Affinity Capture-MS Homo sapiens 14 YWHAB 7529 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 PARD3 56288 Proximity Label-MS Homo sapiens 16 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 17 CTNNB1 1499 Proximity Label-MS Homo sapiens 18 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 19 DNAJA3 9093 Proximity Label-MS Homo sapiens 20 PTEN 5728 Affinity Capture-MS Homo sapiens 21 RAB32 10981 Affinity Capture-MS Homo sapiens 22 RPS6KB2   Affinity Capture-MS Homo sapiens 23 PSMD14 10213 Affinity Capture-MS Homo sapiens 24 YWHAQ 10971 Affinity Capture-MS Homo sapiens 25 CDK2 1017 Affinity Capture-MS Homo sapiens 26 SAAL1 113174 Co-fractionation Homo sapiens 27 CDH1 999 Proximity Label-MS Homo sapiens 28 MYO6 4646 Proximity Label-MS Homo sapiens 29 MLLT4 4301 Proximity Label-MS Homo sapiens 30 BTF3 689 Affinity Capture-MS Homo sapiens 31 MYH9 4627 Affinity Capture-MS Homo sapiens 32 CEMIP 57214 Affinity Capture-MS Homo sapiens 33 CEP135   Proximity Label-MS Homo sapiens 34 PINK1   Affinity Capture-MS Homo sapiens 35 DTX2 113878 Proximity Label-MS Homo sapiens 36 CCDC14   Proximity Label-MS Homo sapiens 37 UBTD1 80019 Co-purification Homo sapiens 38 SERBP1 26135 Affinity Capture-MS Homo sapiens 39 BICD1 636 Proximity Label-MS Homo sapiens 40 SNW1 22938 Affinity Capture-MS Homo sapiens 41 RHOB 388 Proximity Label-MS Homo sapiens 42 ILK 3611 Affinity Capture-MS Homo sapiens 43 HECTD1 25831 Affinity Capture-MS Homo sapiens 44 YWHAG 7532 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 MYC   Affinity Capture-MS Homo sapiens 46 SIK2   Affinity Capture-MS Homo sapiens 47 DDX39B 7919 Affinity Capture-MS Homo sapiens 48 SEC61B 10952 Proximity Label-MS Homo sapiens 49 SPSB4   Affinity Capture-MS Homo sapiens 50 CDC5L 988 Affinity Capture-MS Homo sapiens View the network

Pathways in which SHROOM3 is involved

No pathways found