Gene description for Tpm3
Gene name tropomyosin 3, gamma
Gene symbol Tpm3
Other names/aliases TM30nm
TMnm
Tm5NM
Tpm-5
Tpm5
Trop-5
gamma-TM
hTM30nm
hTMnm
Species Mus musculus
 Database cross references - Tpm3
ExoCarta ExoCarta_59069
Vesiclepedia VP_59069
Entrez Gene 59069
UniProt P21107  
 Tpm3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tpm3
Molecular Function
    protein binding GO:0005515 IPI
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
Biological Process
    muscle contraction GO:0006936 IBA
    actin filament organization GO:0007015 IBA
Subcellular Localization
    stress fiber GO:0001725 ISO
    podosome GO:0002102 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    actin filament GO:0005884 IBA
    actin cytoskeleton GO:0015629 ISO
    growth cone GO:0030426 IDA
    cortical cytoskeleton GO:0030863 IDA
    cleavage furrow GO:0032154 IDA
    neuron projection GO:0043005 IDA
 Experiment description of studies that identified Tpm3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tpm3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Mettl21e  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Tfe3  
Affinity Capture-MS Mus musculus
7 Dbnl 13169
Co-fractionation Mus musculus
8 Tpm4  
Co-fractionation Mus musculus
9 Tmod1  
Co-fractionation Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Tmod2  
Co-fractionation Mus musculus
12 HACE1 57531
Affinity Capture-MS Homo sapiens
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