Gene description for ST3GAL4
Gene name ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Gene symbol ST3GAL4
Other names/aliases CGS23
NANTA3
SAT3
SIAT4
SIAT4C
ST3GalIV
STZ
Species Homo sapiens
 Database cross references - ST3GAL4
ExoCarta ExoCarta_6484
Vesiclepedia VP_6484
Entrez Gene 6484
HGNC 10864
MIM 104240
UniProt Q11206  
 ST3GAL4 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ST3GAL4
Molecular Function
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 IBA
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 IDA
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 TAS
    N-acetyllactosaminide alpha-2,3-sialyltransferase activity GO:0008118 IDA
    N-acetyllactosaminide alpha-2,3-sialyltransferase activity GO:0008118 IMP
    sialyltransferase activity GO:0008373 TAS
    beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase GO:0047288 IBA
    beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase GO:0047288 IDA
Biological Process
    protein glycosylation GO:0006486 IBA
    protein glycosylation GO:0006486 IDA
    glycoprotein biosynthetic process GO:0009101 IDA
    glycolipid biosynthetic process GO:0009247 IBA
    glycolipid biosynthetic process GO:0009247 IDA
    oligosaccharide biosynthetic process GO:0009312 IDA
    oligosaccharide biosynthetic process GO:0009312 TAS
    O-glycan processing GO:0016266 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    viral protein processing GO:0019082 TAS
    positive regulation of blood coagulation GO:0030194 ISS
    lipid glycosylation GO:0030259 IDA
    cognition GO:0050890 IMP
    sialylation GO:0097503 IDA
    positive regulation of leukocyte tethering or rolling GO:1903238 IMP
    protein sialylation GO:1990743 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 IEA
    membrane GO:0016020 HDA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified ST3GAL4 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ST3GAL4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 PCSK1N  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 C1GALT1C1 29071
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LRP5 4041
Affinity Capture-MS Homo sapiens
5 GPIHBP1  
Affinity Capture-MS Homo sapiens
6 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NAAA  
Affinity Capture-MS Homo sapiens
8 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
9 CHRNA4  
Affinity Capture-MS Homo sapiens
10 FAM20C 56975
Affinity Capture-MS Homo sapiens
11 DEFA1 1667
Affinity Capture-MS Homo sapiens
12 CLSTN3 9746
Affinity Capture-MS Homo sapiens
13 LCN6  
Affinity Capture-MS Homo sapiens
14 HFE  
Affinity Capture-MS Homo sapiens
15 TMEM25  
Affinity Capture-MS Homo sapiens
16 MANEAL 149175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PON2 5445
Affinity Capture-MS Homo sapiens
18 FAM213A 84293
Affinity Capture-MS Homo sapiens
19 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PDGFRA 5156
Affinity Capture-MS Homo sapiens
21 FAM3C 10447
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 LRFN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RMND1 55005
Affinity Capture-MS Homo sapiens
24 ADPGK 83440
Affinity Capture-MS Homo sapiens
25 KLRD1  
Affinity Capture-MS Homo sapiens
26 BTN2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 GGT7 2686
Affinity Capture-MS Homo sapiens
28 NDUFA4 4697
Affinity Capture-MS Homo sapiens
29 SCGB1C1  
Affinity Capture-MS Homo sapiens
30 IL5RA  
Affinity Capture-MS Homo sapiens
31 TMX3 54495
Affinity Capture-MS Homo sapiens
32 APEX1 328
Affinity Capture-RNA Homo sapiens
33 MSMB  
Affinity Capture-MS Homo sapiens
34 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CHRNA5  
Affinity Capture-MS Homo sapiens
36 AVP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 LRP6 4040
Affinity Capture-MS Homo sapiens
38 LCN9  
Affinity Capture-MS Homo sapiens
39 SCGB2A2  
Affinity Capture-MS Homo sapiens
40 C1orf54  
Affinity Capture-MS Homo sapiens
41 PSCA 8000
Affinity Capture-MS Homo sapiens
42 CRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 IMPAD1 54928
Affinity Capture-MS Homo sapiens
44 IL27RA 9466
Affinity Capture-MS Homo sapiens
45 DHFRL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 OAF 220323
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CHRNE  
Affinity Capture-MS Homo sapiens
48 SLC31A1 1317
Affinity Capture-MS Homo sapiens
49 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 EDDM3B  
Affinity Capture-MS Homo sapiens
51 TBC1D15 64786
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 TAS1R2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ST3GAL4 is involved
PathwayEvidenceSource
Asparagine N-linked glycosylation TAS Reactome
Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein TAS Reactome
Blood group systems biosynthesis TAS Reactome
Disease IEA Reactome
Disease TAS Reactome
Glycosaminoglycan metabolism TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Keratan sulfate biosynthesis TAS Reactome
Keratan sulfate/keratin metabolism TAS Reactome
Late SARS-CoV-2 Infection Events IEA Reactome
Late SARS-CoV-2 Infection Events TAS Reactome
Lewis blood group biosynthesis TAS Reactome
Maturation of protein 3a TAS Reactome
Maturation of protein 3a IEA Reactome
Maturation of spike protein TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of proteins TAS Reactome
N-Glycan antennae elongation TAS Reactome
N-glycan antennae elongation in the medial/trans-Golgi TAS Reactome
O-linked glycosylation TAS Reactome
O-linked glycosylation of mucins TAS Reactome
Post-translational protein modification TAS Reactome
Pre-NOTCH Expression and Processing IEA Reactome
Pre-NOTCH Processing in Golgi IEA Reactome
SARS-CoV Infections IEA Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-2 Infection IEA Reactome
SARS-CoV-2 Infection TAS Reactome
Sialic acid metabolism TAS Reactome
Signal Transduction IEA Reactome
Signaling by NOTCH IEA Reactome
Synthesis of substrates in N-glycan biosythesis TAS Reactome
Termination of O-glycan biosynthesis TAS Reactome
Translation of Structural Proteins TAS Reactome
Translation of Structural Proteins IEA Reactome
Translation of Structural Proteins TAS Reactome
Transport to the Golgi and subsequent modification TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Infection Pathways TAS Reactome





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