Gene description for VPS33A
Gene name vacuolar protein sorting 33 homolog A (S. cerevisiae)
Gene symbol VPS33A
Other names/aliases -
Species Homo sapiens
 Database cross references - VPS33A
ExoCarta ExoCarta_65082
Vesiclepedia VP_65082
Entrez Gene 65082
HGNC 18179
MIM 610034
UniProt Q96AX1  
 VPS33A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for VPS33A
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    endosome to lysosome transport GO:0008333 IMP
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 IDA
    platelet formation GO:0030220 ISS
    melanosome localization GO:0032400 IDA
    lysosome localization GO:0032418 IDA
    endosomal vesicle fusion GO:0034058 NAS
    regulation of SNARE complex assembly GO:0035542 NAS
    regulation of lysosomal lumen pH GO:0035751 IMP
    regulation of developmental pigmentation GO:0048070 ISS
    autophagosome maturation GO:0097352 IMP
Subcellular Localization
    lysosome GO:0005764 IBA
    lysosomal membrane GO:0005765 HDA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 ISS
    endosome membrane GO:0010008 TAS
    AP-3 adaptor complex GO:0030123 IDA
    clathrin-coated vesicle GO:0030136 IEA
    HOPS complex GO:0030897 IDA
    HOPS complex GO:0030897 NAS
    late endosome membrane GO:0031902 IEA
    CORVET complex GO:0033263 IBA
    CORVET complex GO:0033263 NAS
    perinuclear region of cytoplasm GO:0048471 IDA
    clathrin complex GO:0071439 IDA
 Experiment description of studies that identified VPS33A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS33A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 RPA2 6118
Proximity Label-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 VPS16 64601
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
5 VPS41 27072
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
6 PKNOX1  
Affinity Capture-MS Homo sapiens
7 FBXW11  
Affinity Capture-MS Homo sapiens
8 NPAS1  
Affinity Capture-MS Homo sapiens
9 NSUN5P1  
Affinity Capture-MS Homo sapiens
10 AP3D1 8943
Affinity Capture-Western Homo sapiens
11 SREK1 140890
Co-fractionation Homo sapiens
12 CUL3 8452
Affinity Capture-MS Homo sapiens
13 RAB7A 7879
Proximity Label-MS Homo sapiens
14 LAMTOR1 55004
Proximity Label-MS Homo sapiens
15 VIPAS39 63894
Affinity Capture-Western Homo sapiens
16 LAMP3  
Proximity Label-MS Homo sapiens
17 NR3C1 2908
Proximity Label-MS Homo sapiens
18 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 RAB4A 5867
Proximity Label-MS Homo sapiens
20 Plk2  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
21 RAB5A 5868
Proximity Label-MS Homo sapiens
22 CRYAB 1410
Affinity Capture-MS Homo sapiens
23 TEKT4  
Affinity Capture-MS Homo sapiens
24 FAM96A  
Affinity Capture-MS Homo sapiens
25 CCDC127  
Affinity Capture-MS Homo sapiens
26 D2HGDH 728294
Affinity Capture-MS Homo sapiens
27 INF2 64423
Affinity Capture-MS Homo sapiens
28 UBR7  
Co-fractionation Homo sapiens
29 MON1A 84315
Reconstituted Complex Homo sapiens
30 TRIM35 23087
Affinity Capture-MS Homo sapiens
31 VPS45 11311
Co-fractionation Homo sapiens
32 VPS8 23355
Affinity Capture-MS Homo sapiens
33 SRSF2 6427
Affinity Capture-MS Homo sapiens
34 CLTB 1212
Affinity Capture-Western Homo sapiens
35 NRIP1 8204
Affinity Capture-MS Homo sapiens
36 CLTA 1211
Co-fractionation Homo sapiens
37 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
38 UVRAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 UFL1 23376
Affinity Capture-MS Homo sapiens
40 MON1B 22879
Reconstituted Complex Homo sapiens
41 TECPR2  
Affinity Capture-MS Homo sapiens
42 C12orf74  
Affinity Capture-MS Homo sapiens
43 MTMR7  
Affinity Capture-MS Homo sapiens
44 LRRC29  
Affinity Capture-MS Homo sapiens
45 LAMP2 3920
Proximity Label-MS Homo sapiens
46 NT5C 30833
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 EGFR 1956
Negative Genetic Homo sapiens
49 RAB9A 9367
Proximity Label-MS Homo sapiens
50 CT55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TOLLIP 54472
Proximity Label-MS Homo sapiens
52 ZUFSP 221302
Affinity Capture-MS Homo sapiens
53 UBXN6 80700
Affinity Capture-MS Homo sapiens
54 BICD2 23299
Affinity Capture-MS Homo sapiens
55 VPS11 55823
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
56 PPM1M  
Affinity Capture-MS Homo sapiens
57 Vps41  
Affinity Capture-MS Mus musculus
58 VIM 7431
Co-fractionation Homo sapiens
59 VCPIP1 80124
Affinity Capture-MS Homo sapiens
60 TRMU  
Affinity Capture-MS Homo sapiens
61 PMM1 5372
Affinity Capture-MS Homo sapiens
62 STX17 55014
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
63 VPS18 57617
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
64 C9orf72  
Affinity Capture-MS Homo sapiens
65 HSPB8 26353
Affinity Capture-MS Homo sapiens
66 GALNT9  
Affinity Capture-MS Homo sapiens
67 CDC5L 988
Affinity Capture-MS Homo sapiens
68 BRD4 23476
Affinity Capture-MS Homo sapiens
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