Gene description for SQLE
Gene name squalene epoxidase
Gene symbol SQLE
Other names/aliases -
Species Homo sapiens
 Database cross references - SQLE
ExoCarta ExoCarta_6713
Vesiclepedia VP_6713
Entrez Gene 6713
HGNC 11279
MIM 602019
UniProt Q14534  
 SQLE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SQLE
Molecular Function
    squalene monooxygenase activity GO:0004506 IBA
    squalene monooxygenase activity GO:0004506 IDA
    squalene monooxygenase activity GO:0004506 NAS
    protein binding GO:0005515 IPI
    FAD binding GO:0071949 IDA
Biological Process
    cholesterol metabolic process GO:0008203 IBA
    sterol biosynthetic process GO:0016126 IBA
    sterol biosynthetic process GO:0016126 IDA
    sterol biosynthetic process GO:0016126 NAS
    regulation of cell population proliferation GO:0042127 IMP
    lipid droplet formation GO:0140042 IMP
    response to biphenyl GO:1904614 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified SQLE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SQLE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OR2M7  
Affinity Capture-MS Homo sapiens
2 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 C7orf34  
Affinity Capture-MS Homo sapiens
4 SLC17A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 OR6T1  
Affinity Capture-MS Homo sapiens
6 ALDOC 230
Two-hybrid Homo sapiens
7 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KCNA2  
Affinity Capture-MS Homo sapiens
9 PCDHGA5  
Affinity Capture-MS Homo sapiens
10 ZDHHC12  
Affinity Capture-MS Homo sapiens
11 PTCH2  
Affinity Capture-MS Homo sapiens
12 TMEM14B  
Two-hybrid Homo sapiens
13 RNFT1  
Affinity Capture-MS Homo sapiens
14 ZNF641  
Two-hybrid Homo sapiens
15 REEP4  
Two-hybrid Homo sapiens
16 AMFR 267
Affinity Capture-MS Homo sapiens
17 CREB3L1  
Two-hybrid Homo sapiens
18 C3orf52  
Affinity Capture-MS Homo sapiens
19 CREB3  
Two-hybrid Homo sapiens
20 C16orf58 64755
Affinity Capture-MS Homo sapiens
21 CYB5B 80777
Affinity Capture-MS Homo sapiens
22 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SLC30A2  
Affinity Capture-MS Homo sapiens
25 LDLR 3949
Negative Genetic Homo sapiens
26 MARCH4  
Affinity Capture-MS Homo sapiens
27 TREML2 79865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SLC18A1  
Affinity Capture-MS Homo sapiens
29 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 RNF185  
Affinity Capture-MS Homo sapiens
31 PIGH  
Affinity Capture-MS Homo sapiens
32 TMCO3 55002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PCDHGC4  
Affinity Capture-MS Homo sapiens
34 MARCH6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 RAN 5901
Affinity Capture-MS Homo sapiens
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