Gene description for C8A
Gene name complement component 8, alpha polypeptide
Gene symbol C8A
Other names/aliases -
Species Homo sapiens
 Database cross references - C8A
ExoCarta ExoCarta_731
Vesiclepedia VP_731
Entrez Gene 731
HGNC 1352
MIM 120950
UniProt P07357  
 C8A identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for C8A
Molecular Function
    complement binding GO:0001848 IEA
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IEA
Biological Process
    immune response GO:0006955 TAS
    complement activation GO:0006956 IBA
    complement activation GO:0006956 IDA
    complement activation, alternative pathway GO:0006957 IEA
    complement activation, classical pathway GO:0006958 IEA
    killing of cells of another organism GO:0031640 IEA
    positive regulation of immune response GO:0050778 NAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    membrane attack complex GO:0005579 IBA
    membrane attack complex GO:0005579 IDA
    membrane attack complex GO:0005579 IPI
    extracellular space GO:0005615 IBA
    plasma membrane GO:0005886 NAS
    membrane GO:0016020 TAS
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified C8A in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C8A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MESDC2 23184
Affinity Capture-MS Homo sapiens
2 CMIP 80790
Affinity Capture-MS Homo sapiens
3 MMGT1 93380
Two-hybrid Homo sapiens
4 C8G 733
Reconstituted Complex Homo sapiens
5 RSPH4A  
Affinity Capture-MS Homo sapiens
6 TAF7  
Affinity Capture-MS Homo sapiens
7 C8B 732
Cross-Linking-MS (XL-MS) Homo sapiens
8 IDE 3416
Affinity Capture-MS Homo sapiens
9 GOLT1A  
Two-hybrid Homo sapiens
10 GPR152  
Two-hybrid Homo sapiens
11 CCR1  
Affinity Capture-MS Homo sapiens
12 FBXO2 26232
Affinity Capture-MS Homo sapiens
13 ZMYM2  
Affinity Capture-MS Homo sapiens
14 MMP14 4323
Affinity Capture-MS Homo sapiens
15 HSPA5 3309
Affinity Capture-MS Homo sapiens
16 CD59 966
Reconstituted Complex Homo sapiens
17 SLC10A1  
Two-hybrid Homo sapiens
18 SLC7A1 6541
Two-hybrid Homo sapiens
19 EEF1A1 1915
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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