Gene description for FTO
Gene name fat mass and obesity associated
Gene symbol FTO
Other names/aliases ALKBH9
Species Homo sapiens
 Database cross references - FTO
ExoCarta ExoCarta_79068
Vesiclepedia VP_79068
Entrez Gene 79068
HGNC 24678
MIM 610966
UniProt Q9C0B1  
 FTO identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FTO
Molecular Function
    protein binding GO:0005515 IPI
    ferrous iron binding GO:0008198 IBA
    ferrous iron binding GO:0008198 IDA
    transferase activity GO:0016740 IEA
    oxidative RNA demethylase activity GO:0035515 IDA
    broad specificity oxidative DNA demethylase activity GO:0035516 IBA
    broad specificity oxidative DNA demethylase activity GO:0035516 IDA
    mRNA N6-methyladenosine dioxygenase activity GO:1990931 IBA
    mRNA N6-methyladenosine dioxygenase activity GO:1990931 IDA
    mRNA N6-methyladenosine dioxygenase activity GO:1990931 IMP
    tRNA demethylase activity GO:1990984 IDA
Biological Process
    temperature homeostasis GO:0001659 IEA
    DNA alkylation repair GO:0006307 IDA
    regulation of lipid storage GO:0010883 IMP
    snRNA processing GO:0016180 IDA
    regulation of multicellular organism growth GO:0040014 IEA
    RNA repair GO:0042245 IDA
    regulation of respiratory system process GO:0044065 IEA
    adipose tissue development GO:0060612 IEA
    mRNA destabilization GO:0061157 IDA
    regulation of white fat cell proliferation GO:0070350 IEA
    regulation of brown fat cell differentiation GO:0090335 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    nuclear speck GO:0016607 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified FTO in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FTO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MORN3  
Two-hybrid Homo sapiens
2 SPANXN5  
Affinity Capture-MS Homo sapiens
3 LDHA 3939
Co-fractionation Homo sapiens
4 NPEPPS 9520
Co-fractionation Homo sapiens
5 GNG2 54331
Affinity Capture-MS Homo sapiens
6 GNG13  
Two-hybrid Homo sapiens
7 MAGEF1  
Affinity Capture-MS Homo sapiens
8 SPRED2 200734
Affinity Capture-MS Homo sapiens
9 MVD 4597
Co-fractionation Homo sapiens
10 GPX7 2882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ANKRD11 29123
Two-hybrid Homo sapiens
12 MTPN 136319
Affinity Capture-MS Homo sapiens
13 PRNP 5621
Affinity Capture-MS Homo sapiens
14 LDHB 3945
Co-fractionation Homo sapiens
15 SDC1 6382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ZNF232  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CCDC57 284001
Two-hybrid Homo sapiens
18 ZMAT3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
20 CLP1  
Two-hybrid Homo sapiens
21 CDKN2AIP  
Affinity Capture-MS Homo sapiens
22 CARTPT  
Affinity Capture-MS Homo sapiens
23 DIRAS2 54769
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SLC9A3R1 9368
Co-fractionation Homo sapiens
25 NAT1 9
Affinity Capture-MS Homo sapiens
26 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TYW3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FSD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 AMD1  
Affinity Capture-Western Homo sapiens
30 OSGEP 55644
Affinity Capture-MS Homo sapiens
31 IL16  
Two-hybrid Homo sapiens
32 SBF2 81846
Proximity Label-MS Homo sapiens
33 EFEMP2 30008
Two-hybrid Homo sapiens
34 C19orf25  
Affinity Capture-MS Homo sapiens
35 BCCIP 56647
Co-fractionation Homo sapiens
36 DDX58 23586
Affinity Capture-RNA Homo sapiens
37 RIPK4  
Affinity Capture-MS Homo sapiens
38 ZNF77  
Two-hybrid Homo sapiens
39 KLHL38  
Two-hybrid Homo sapiens
40 OR5F1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 NXF1 10482
Two-hybrid Homo sapiens
42 EEF2 1938
Co-fractionation Homo sapiens
43 CKB 1152
Co-fractionation Homo sapiens
44 HOXC5  
Affinity Capture-MS Homo sapiens
45 CTSA 5476
Co-fractionation Homo sapiens
46 GPANK1  
Two-hybrid Homo sapiens
47 CRCP  
Affinity Capture-MS Homo sapiens
48 TNPO2 30000
Two-hybrid Homo sapiens
49 RPA3 6119
Proximity Label-MS Homo sapiens
50 OGT 8473
Reconstituted Complex Homo sapiens
51 NDRG1 10397
Co-fractionation Homo sapiens
52 C1orf109  
Two-hybrid Homo sapiens
53 MPZL1 9019
Affinity Capture-MS Homo sapiens
54 SLC2A5 6518
Affinity Capture-MS Homo sapiens
55 IQGAP1 8826
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 SPANXN4  
Affinity Capture-MS Homo sapiens
57 TULP3 7289
Affinity Capture-MS Homo sapiens
58 PGAP2  
Affinity Capture-MS Homo sapiens
59 SHC3  
Two-hybrid Homo sapiens
60 TKFC 26007
Co-fractionation Homo sapiens
61 NFYA 4800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ENTPD7  
Affinity Capture-MS Homo sapiens
63 RNF144A  
Affinity Capture-MS Homo sapiens
64 ALS2CR12  
Affinity Capture-MS Homo sapiens
65 GNG8  
Affinity Capture-MS Homo sapiens
66 USP20  
Two-hybrid Homo sapiens
67 PEPD 5184
Co-fractionation Homo sapiens
68 ALX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 MAD2L2 10459
Two-hybrid Homo sapiens
70 CNDP2 55748
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which FTO is involved
PathwayEvidenceSource
DNA Damage Reversal TAS Reactome
DNA Repair TAS Reactome
Reversal of alkylation damage by DNA dioxygenases TAS Reactome





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