Gene description for C2orf49
Gene name chromosome 2 open reading frame 49
Gene symbol C2orf49
Other names/aliases asw
Species Homo sapiens
 Database cross references - C2orf49
ExoCarta ExoCarta_79074
Vesiclepedia VP_79074
Entrez Gene 79074
HGNC 28772
UniProt Q9BVC5  
 C2orf49 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for C2orf49
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 NAS
    embryonic morphogenesis GO:0048598 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 NAS
    tRNA-splicing ligase complex GO:0072669 IBA
    tRNA-splicing ligase complex GO:0072669 IDA
    tRNA-splicing ligase complex GO:0072669 NAS
 Experiment description of studies that identified C2orf49 in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for C2orf49
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MSGN1  
Affinity Capture-MS Homo sapiens
2 HNRNPD 3184
Co-fractionation Homo sapiens
3 MYCN  
Affinity Capture-MS Homo sapiens
4 PIN1 5300
Affinity Capture-MS Homo sapiens
5 YWHAE 7531
Affinity Capture-MS Homo sapiens
6 YWHAZ 7534
Affinity Capture-MS Homo sapiens
7 EGFR 1956
Affinity Capture-MS Homo sapiens
8 FUS 2521
Co-fractionation Homo sapiens
9 TMOD1  
Affinity Capture-MS Homo sapiens
10 APP 351
Reconstituted Complex Homo sapiens
11 TRIM54  
Two-hybrid Homo sapiens
12 ANO4 121601
Affinity Capture-MS Homo sapiens
13 MICU2 221154
Affinity Capture-MS Homo sapiens
14 ACTR6  
Affinity Capture-MS Homo sapiens
15 E2F4  
Affinity Capture-MS Homo sapiens
16 MYC  
Affinity Capture-MS Homo sapiens
17 GLI2  
Affinity Capture-MS Homo sapiens
18 RTCB 51493
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
19 C14orf166 51637
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
20 RNASE1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ASCL3  
Affinity Capture-MS Homo sapiens
22 YWHAQ 10971
Affinity Capture-MS Homo sapiens
23 MDC1  
Affinity Capture-MS Homo sapiens
24 YWHAG 7532
Affinity Capture-MS Homo sapiens
25 STYX  
Affinity Capture-MS Homo sapiens
26 DDX1 1653
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
27 DDX39B 7919
Affinity Capture-MS Homo sapiens
28 PRMT1 3276
Affinity Capture-MS Homo sapiens
29 CEBPA  
Protein-peptide Homo sapiens
30 FAM98B 283742
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which C2orf49 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome





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