ExoCarta: Gene summary

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Gene description for THSD4
Gene name	thrombospondin, type I, domain containing 4
Gene symbol	THSD4
Other names/aliases	ADAMTSL-6 ADAMTSL6 FVSY9334 PRO34005
Species	Homo sapiens
Database cross references - THSD4
ExoCarta	ExoCarta_79875
Vesiclepedia	VP_79875
Entrez Gene	79875
HGNC	25835
MIM	614476
UniProt	Q6ZMP0
THSD4 identified in sEVs derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Mesenchymal stem cells	36408942
Saliva	19199708

Gene ontology annotations for THSD4

Molecular Function
	extracellular matrix structural constituent	GO:0005201	RCA
	hydrolase activity	GO:0016787	IEA
Biological Process
	elastic fiber assembly	GO:0048251	IEA
	microfibril assembly	GO:0160054	IMP
Subcellular Localization
	microfibril	GO:0001527	IMP
	collagen-containing extracellular matrix	GO:0062023	HDA
	extracellular exosome	GO:0070062	HDA

Experiment description of studies that identified THSD4 in sEVs

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

MISEV standards
✔ IEM	Biophysical techniques
✔ TSG101\|Alix\|CD63\|CD81	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

19199708

Organism

Homo sapiens

Experiment description

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

Authors

"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"

Journal name

JPR

Publication year

2009

Sample

Saliva

Sample name

Saliva

Isolation/purification methods

Differential centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy

Protein-protein interactions for THSD4

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

LY86

Affinity Capture-MS

Homo sapiens

NPTX1

Affinity Capture-MS

Homo sapiens

BTNL2

Affinity Capture-MS

Homo sapiens

EPDR1

54749

Affinity Capture-MS

Homo sapiens

BCAN

Affinity Capture-MS

Homo sapiens

ARID5B

84159

Affinity Capture-MS

Homo sapiens

FOXG1

Affinity Capture-MS

Homo sapiens

HSPA8

3312

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ALMS1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

UQCC1

55245

Affinity Capture-MS

Homo sapiens

KANSL1

Affinity Capture-MS

Homo sapiens

HOXD13

Affinity Capture-MS

Homo sapiens

GGH

8836

Affinity Capture-MS

Homo sapiens

NUFIP2

57532

Affinity Capture-MS

Homo sapiens

CFC1

Affinity Capture-MS

Homo sapiens

MLF1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRG2

Affinity Capture-MS

Homo sapiens

MFAP4

4239

Affinity Capture-MS

Homo sapiens

PI15

Affinity Capture-MS

Homo sapiens

VSIG8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FHL3

2275

Affinity Capture-MS

Homo sapiens

USP54

159195

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LOXL4

84171

Affinity Capture-MS

Homo sapiens

RIPK1

8737

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NCR3

Affinity Capture-MS

Homo sapiens

KRBA1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ELSPBP1

Affinity Capture-MS

Homo sapiens

APPBP2

Affinity Capture-MS

Homo sapiens

OFD1

8481

Affinity Capture-MS

Homo sapiens

ZNF195

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

WDR76

Affinity Capture-MS

Homo sapiens

TRIM68

55128

Affinity Capture-MS

Homo sapiens

ZNF281

Affinity Capture-MS

Homo sapiens

SDF2L1

23753

Affinity Capture-MS

Homo sapiens

HSPA2

3306

Affinity Capture-MS

Homo sapiens

FBXO2

26232

Affinity Capture-MS

Homo sapiens

HELZ

9931

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GLI3

2737

Affinity Capture-MS

Homo sapiens

KANK2

25959

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DNAJB9

4189

Affinity Capture-MS

Homo sapiens

CEP164

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ETV3

Affinity Capture-MS

Homo sapiens

CDHR4

Affinity Capture-MS

Homo sapiens

EDN3

Affinity Capture-MS

Homo sapiens

DNASE1L1

1774

Affinity Capture-MS

Homo sapiens

NXPH3

Affinity Capture-MS

Homo sapiens

ZNF609

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which THSD4 is involved

Pathway	Evidence	Source
Defective B3GALTL causes PpS	TAS	Reactome
Disease	TAS	Reactome
Diseases associated with O-glycosylation of proteins	TAS	Reactome
Diseases of glycosylation	TAS	Reactome
Diseases of metabolism	TAS	Reactome
Metabolism of proteins	TAS	Reactome
O-glycosylation of TSR domain-containing proteins	TAS	Reactome
O-linked glycosylation	TAS	Reactome
Post-translational protein modification	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here