Gene description for PITPNM3
Gene name PITPNM family member 3
Gene symbol PITPNM3
Other names/aliases ACKR6
CORD5
NIR1
RDGBA3
Species Homo sapiens
 Database cross references - PITPNM3
ExoCarta ExoCarta_83394
Vesiclepedia VP_83394
Entrez Gene 83394
HGNC 21043
MIM 608921
UniProt Q9BZ71  
 PITPNM3 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PITPNM3
Molecular Function
    phospholipase activity GO:0004620 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    phosphatidylinositol transfer activity GO:0008526 TAS
    receptor tyrosine kinase binding GO:0030971 IMP
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    phospholipid transport GO:0015914 IEA
    intermembrane lipid transfer GO:0120009 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 IEA
    cell projection GO:0042995 IEA
    cell body GO:0044297 IEA
 Experiment description of studies that identified PITPNM3 in sEVs
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PITPNM3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 KRAS 3845
Synthetic Lethality Homo sapiens
3 OSBPL3 26031
Affinity Capture-MS Homo sapiens
4 NR3C1 2908
Proximity Label-MS Homo sapiens
5 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CKAP5 9793
Affinity Capture-MS Homo sapiens
7 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ZFPL1 7542
Affinity Capture-MS Homo sapiens
9 PTK2B 2185
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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