Experiment description of studies that identified TSPAN10 in sEVs

1 Experiment ID 254 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name MNT-1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 255 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name G1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 256 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 501mel Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 257 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name Daju Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for TSPAN10

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ADAM10 102 Affinity Capture-Western Homo sapiens 2 SPPL3   Affinity Capture-MS Homo sapiens 3 CSNK1G3 1456 Affinity Capture-MS Homo sapiens 4 GLS 2744 Affinity Capture-MS Homo sapiens 5 OR52B2   Affinity Capture-MS Homo sapiens 6 C4orf32   Affinity Capture-MS Homo sapiens 7 GNPAT 8443 Affinity Capture-MS Homo sapiens 8 AGPAT4 56895 Affinity Capture-MS Homo sapiens 9 RTN2 6253 Affinity Capture-MS Homo sapiens 10 AGPAT9   Affinity Capture-MS Homo sapiens 11 KIF14 9928 Affinity Capture-MS Homo sapiens 12 NRSN2   Affinity Capture-MS Homo sapiens 13 TSC2 7249 Affinity Capture-MS Homo sapiens 14 FITM2   Affinity Capture-MS Homo sapiens 15 GJA1 2697 Affinity Capture-MS Homo sapiens 16 ECT2 1894 Affinity Capture-MS Homo sapiens 17 UQCRH 7388 Affinity Capture-MS Homo sapiens 18 SLC26A6 65010 Affinity Capture-MS Homo sapiens 19 VEZT 55591 Affinity Capture-MS Homo sapiens 20 MTCH2 23788 Affinity Capture-MS Homo sapiens 21 CYP2S1   Affinity Capture-MS Homo sapiens 22 ATP12A 479 Affinity Capture-MS Homo sapiens 23 LPCAT4   Affinity Capture-MS Homo sapiens 24 SLC26A2 1836 Affinity Capture-MS Homo sapiens 25 KIF20A 10112 Affinity Capture-MS Homo sapiens 26 OXSR1 9943 Affinity Capture-MS Homo sapiens 27 TMEM186   Affinity Capture-MS Homo sapiens 28 LAMP2 3920 Proximity Label-MS Homo sapiens 29 GHDC   Affinity Capture-MS Homo sapiens 30 GPR114   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 KLRG2 346689 Affinity Capture-MS Homo sapiens 32 TMEM11 8834 Affinity Capture-MS Homo sapiens 33 ARL8B 55207 Affinity Capture-MS Homo sapiens 34 AURKB 9212 Affinity Capture-MS Homo sapiens 35 GPD2 2820 Affinity Capture-MS Homo sapiens 36 FAM134C 162427 Affinity Capture-MS Homo sapiens 37 HSD17B13   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 GOLGA5 9950 Affinity Capture-MS Homo sapiens 39 ATP9A 10079 Affinity Capture-MS Homo sapiens 40 OR6T1   Affinity Capture-MS Homo sapiens 41 ELOVL2   Affinity Capture-MS Homo sapiens 42 RAB9A 9367 Affinity Capture-MS Homo sapiens 43 KIAA1524 57650 Affinity Capture-MS Homo sapiens 44 CISD2 493856 Affinity Capture-MS Homo sapiens 45 SLC7A2 6542 Affinity Capture-MS Homo sapiens 46 LHFPL1   Affinity Capture-MS Homo sapiens 47 TMEM205 374882 Affinity Capture-MS Homo sapiens 48 PRAF2 11230 Affinity Capture-MS Homo sapiens 49 SLC1A1 6505 Affinity Capture-MS Homo sapiens 50 AP3D1 8943 Affinity Capture-MS Homo sapiens 51 ALG11   Affinity Capture-MS Homo sapiens 52 ADIPOR1 51094 Affinity Capture-MS Homo sapiens 53 ELOVL5 60481 Affinity Capture-MS Homo sapiens 54 SPTLC2 9517 Affinity Capture-MS Homo sapiens 55 SC5D 6309 Affinity Capture-MS Homo sapiens 56 EIF2B4 8890 Affinity Capture-MS Homo sapiens 57 ATP13A1 57130 Affinity Capture-MS Homo sapiens 58 FAM210B   Affinity Capture-MS Homo sapiens 59 PNLDC1   Affinity Capture-MS Homo sapiens View the network

Pathways in which TSPAN10 is involved

No pathways found