Experiment description of studies that identified FRMD5 in sEVs

1 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for FRMD5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YWHAH 7533 Affinity Capture-MS Homo sapiens 2 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 3 FTL 2512 Affinity Capture-MS Homo sapiens 4 PCDHA8   Affinity Capture-MS Homo sapiens 5 CYP26B1   Affinity Capture-MS Homo sapiens 6 IL17RA 23765 Affinity Capture-MS Homo sapiens 7 BTNL2   Affinity Capture-MS Homo sapiens 8 COLEC12 81035 Affinity Capture-MS Homo sapiens 9 NRCAM   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 STX12 23673 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 CIB2   Affinity Capture-MS Homo sapiens 12 BTNL9   Affinity Capture-MS Homo sapiens 13 CIT 11113 Affinity Capture-MS Homo sapiens 14 SRC 6714 Affinity Capture-MS Homo sapiens 15 OPALIN   Affinity Capture-MS Homo sapiens 16 LDLRAD1   Affinity Capture-MS Homo sapiens 17 SMIM5   Affinity Capture-MS Homo sapiens 18 BAG5 9529 Affinity Capture-MS Homo sapiens 19 CLEC2D   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 PIGH   Affinity Capture-MS Homo sapiens 21 SSSCA1 10534 Affinity Capture-MS Homo sapiens 22 CD6 923 Affinity Capture-MS Homo sapiens 23 ECEL1   Affinity Capture-MS Homo sapiens 24 YWHAB 7529 Affinity Capture-MS Homo sapiens 25 TMEM9B 56674 Affinity Capture-MS Homo sapiens 26 CLEC4A   Affinity Capture-MS Homo sapiens 27 KLRG2 346689 Affinity Capture-MS Homo sapiens 28 RPS17 6218 Affinity Capture-MS Homo sapiens 29 SGCB 6443 Affinity Capture-MS Homo sapiens 30 KIAA1467 57613 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 VSIG4   Affinity Capture-MS Homo sapiens 32 KCNE3   Affinity Capture-MS Homo sapiens 33 TGFA   Affinity Capture-MS Homo sapiens 34 C3orf52   Affinity Capture-MS Homo sapiens 35 S1PR1 1901 Affinity Capture-MS Homo sapiens 36 PCDHB3   Affinity Capture-MS Homo sapiens 37 CCDC107   Affinity Capture-MS Homo sapiens 38 LPAR2 9170 Affinity Capture-MS Homo sapiens 39 FAM96A   Affinity Capture-MS Homo sapiens 40 EVA1B 55194 Affinity Capture-MS Homo sapiens 41 DNAJA1 3301 Affinity Capture-MS Homo sapiens 42 PTPRN   Affinity Capture-MS Homo sapiens 43 PCDHA12   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 YWHAQ 10971 Affinity Capture-MS Homo sapiens 45 ENPEP 2028 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 TUBA1C 84790 Affinity Capture-MS Homo sapiens 47 RCN2 5955 Affinity Capture-MS Homo sapiens 48 MPPE1 65258 Affinity Capture-MS Homo sapiens 49 HSPB8 26353 Affinity Capture-MS Homo sapiens 50 PTGES3 10728 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 YWHAG 7532 Affinity Capture-MS Homo sapiens 52 S1PR4   Affinity Capture-MS Homo sapiens 53 GRAMD1B   Affinity Capture-MS Homo sapiens 54 SPINT2 10653 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 CLEC4E   Affinity Capture-MS Homo sapiens 56 PRKAR1A 5573 Affinity Capture-MS Homo sapiens 57 ASB14   Affinity Capture-MS Homo sapiens 58 ACTL6B   Affinity Capture-MS Homo sapiens 59 PCDHAC2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 CACYBP 27101 Affinity Capture-MS Homo sapiens 61 MRVI1 10335 Affinity Capture-MS Homo sapiens View the network

Pathways in which FRMD5 is involved

No pathways found