Gene description for ALDH4A1
Gene name aldehyde dehydrogenase 4 family, member A1
Gene symbol ALDH4A1
Other names/aliases ALDH4
P5CD
P5CDh
Species Homo sapiens
 Database cross references - ALDH4A1
ExoCarta ExoCarta_8659
Vesiclepedia VP_8659
Entrez Gene 8659
HGNC 406
MIM 606811
UniProt P30038  
 ALDH4A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ALDH4A1
Molecular Function
    1-pyrroline-5-carboxylate dehydrogenase activity GO:0003842 IBA
    aldehyde dehydrogenase (NAD+) activity GO:0004029 IDA
    protein binding GO:0005515 IPI
    electron transfer activity GO:0009055 TAS
    identical protein binding GO:0042802 IPI
Biological Process
    proline metabolic process GO:0006560 TAS
    proline catabolic process GO:0006562 TAS
    proline catabolic process to glutamate GO:0010133 IEA
    4-hydroxyproline catabolic process GO:0019470 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified ALDH4A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ALDH4A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPST 4357
Co-fractionation Homo sapiens
2 GSTP1 2950
Co-fractionation Homo sapiens
3 FABP3 2170
Co-fractionation Homo sapiens
4 LDHA 3939
Co-fractionation Homo sapiens
5 ISG15 9636
Co-fractionation Homo sapiens
6 ESD 2098
Co-fractionation Homo sapiens
7 GLRX5 51218
Co-fractionation Homo sapiens
8 MSRB2  
Co-fractionation Homo sapiens
9 LDHB 3945
Co-fractionation Homo sapiens
10 LAP3 51056
Co-fractionation Homo sapiens
11 TMEM184A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 OIT3 170392
Affinity Capture-MS Homo sapiens
13 CFL1 1072
Co-fractionation Homo sapiens
14 ALDH7A1 501
Co-fractionation Homo sapiens
15 FFAR1  
Affinity Capture-MS Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 CRYZ 1429
Co-fractionation Homo sapiens
18 PMPCA 23203
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
19 PTRHD1 391356
Co-fractionation Homo sapiens
20 YARS2  
Affinity Capture-MS Homo sapiens
21 HINT2 84681
Proximity Label-MS Homo sapiens
22 FTSJ2 29960
Affinity Capture-MS Homo sapiens
23 FXN  
Co-fractionation Homo sapiens
24 MALSU1  
Affinity Capture-MS Homo sapiens
25 LRRC46  
Affinity Capture-MS Homo sapiens
26 LDHAL6A  
Co-fractionation Homo sapiens
27 PMPCB 9512
Proximity Label-MS Homo sapiens
28 TACO1  
Proximity Label-MS Homo sapiens
29 MDH2 4191
Proximity Label-MS Homo sapiens
30 C6orf203  
Proximity Label-MS Homo sapiens
31 ACSM5 54988
Affinity Capture-MS Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 PPIL1 51645
Co-fractionation Homo sapiens
34 HRSP12 10247
Co-fractionation Homo sapiens
35 TUFM 7284
Proximity Label-MS Homo sapiens
36 PGM2 55276
Co-fractionation Homo sapiens
37 ARG1 383
Two-hybrid Homo sapiens
38 ALDH6A1 4329
Co-fractionation Homo sapiens
39 OGT 8473
Reconstituted Complex Homo sapiens
40 HMGCL 3155
Co-fractionation Homo sapiens
41 TRUB2  
Proximity Label-MS Homo sapiens
42 MTRF1L  
Proximity Label-MS Homo sapiens
43 CLIC4 25932
Co-fractionation Homo sapiens
44 INHBA 3624
Co-fractionation Homo sapiens
45 COX4I1 1327
Co-fractionation Homo sapiens
46 ENO3 2027
Co-fractionation Homo sapiens
47 NIT2 56954
Co-fractionation Homo sapiens
48 ADH5 128
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
49 DCXR 51181
Co-fractionation Homo sapiens
50 AHCY 191
Co-fractionation Homo sapiens
51 PYCR1 5831
Affinity Capture-MS Homo sapiens
52 FAHD1 81889
Affinity Capture-MS Homo sapiens
53 TST 7263
Co-fractionation Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 STK16 8576
Affinity Capture-MS Homo sapiens
56 SELENBP1 8991
Co-fractionation Homo sapiens
57 GPR45  
Affinity Capture-MS Homo sapiens
58 TSFM 10102
Proximity Label-MS Homo sapiens
59 CFL2 1073
Co-fractionation Homo sapiens
60 AUH 549
Proximity Label-MS Homo sapiens
61 DSTN 11034
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
62 ENO1 2023
Co-fractionation Homo sapiens
63 TBCA 6902
Co-fractionation Homo sapiens
64 RAB33A 9363
Affinity Capture-MS Homo sapiens
65 AK4 205
Affinity Capture-MS Homo sapiens
66 CLPP 8192
Proximity Label-MS Homo sapiens
67 CS 1431
Proximity Label-MS Homo sapiens
68 GOT2 2806
Co-fractionation Homo sapiens
69 CLIC1 1192
Co-fractionation Homo sapiens
70 LDHAL6B 92483
Co-fractionation Homo sapiens
71 SOD2 6648
Co-fractionation Homo sapiens
72 ALDH4A1 8659
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
73 MTPN 136319
Co-fractionation Homo sapiens
74 GPI 2821
Co-fractionation Homo sapiens
75 LYPLAL1 127018
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
76 TKT 7086
Co-fractionation Homo sapiens
77 FKBP1A 2280
Co-fractionation Homo sapiens
78 C21orf33  
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
79 SSBP1 6742
Proximity Label-MS Homo sapiens
80 PMM2 5373
Co-fractionation Homo sapiens
81 ANXA11 311
Co-fractionation Homo sapiens
82 IDH2 3418
Co-fractionation Homo sapiens
83 SEC14L2  
Co-fractionation Homo sapiens
84 TBRG4 9238
Proximity Label-MS Homo sapiens
85 ARRB1 408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 GOT1 2805
Co-fractionation Homo sapiens
87 ACO2 50
Co-fractionation Homo sapiens
88 C1QBP 708
Proximity Label-MS Homo sapiens
89 QRSL1  
Affinity Capture-MS Homo sapiens
90 OR2A4 79541
Affinity Capture-MS Homo sapiens
91 METTL17  
Proximity Label-MS Homo sapiens
92 CCR1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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