Experiment description of studies that identified ASAP2 in sEVs

1 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for ASAP2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 KNDC1   Affinity Capture-MS Homo sapiens 2 THSD7A 221981 Two-hybrid Homo sapiens 3 SEC24A 10802 Co-fractionation Homo sapiens 4 NCK1 4690 Affinity Capture-MS Homo sapiens 5 PARP1 142 Proximity Label-MS Homo sapiens 6 ITSN1 6453 Two-hybrid Homo sapiens 7 GRB2 2885 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 PDAP1 11333 Co-fractionation Homo sapiens 9 CLUAP1 23059 Affinity Capture-MS Homo sapiens 10 APC   Reconstituted Complex Homo sapiens 11 ARHGAP12   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 Asap1   Two-hybrid Mus musculus 13 ASAP3 55616 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 ADD3 120 Co-fractionation Homo sapiens 15 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 16 NCK2 8440 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 DHX40   Co-fractionation Homo sapiens 18 MAP1LC3A 84557 Affinity Capture-MS Homo sapiens 19 TBRG4 9238 Affinity Capture-MS Homo sapiens 20 Reps2   Affinity Capture-Western Mus musculus 21 NTRK1 4914 Affinity Capture-MS Homo sapiens 22 PXN 5829 Reconstituted Complex Homo sapiens 23 PTK2B 2185 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 24 GAPDH 2597 Cross-Linking-MS (XL-MS) Homo sapiens 25 SRC 6714 Affinity Capture-Western Homo sapiens 26 SH3KBP1 30011 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 27 SRM 6723 Co-fractionation Homo sapiens 28 RAB11FIP3   Affinity Capture-Western Homo sapiens 29 C19orf35   Affinity Capture-MS Homo sapiens 30 KIAA1522 57648 Affinity Capture-MS Homo sapiens View the network

Pathways in which ASAP2 is involved

No pathways found