Gene description for PLXNA4
Gene name plexin A4
Gene symbol PLXNA4
Other names/aliases FAYV2820
PLEXA4
PLXNA4A
PLXNA4B
PRO34003
Species Homo sapiens
 Database cross references - PLXNA4
ExoCarta ExoCarta_91584
Vesiclepedia VP_91584
Entrez Gene 91584
HGNC 9102
MIM 604280
UniProt Q9HCM2  
 PLXNA4 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PLXNA4
Molecular Function
    protein binding GO:0005515 IPI
    semaphorin receptor activity GO:0017154 IBA
Biological Process
    axon guidance GO:0007411 IBA
    synapse assembly GO:0007416 IBA
    motor neuron axon guidance GO:0008045 ISS
    facial nerve structural organization GO:0021612 ISS
    glossopharyngeal nerve morphogenesis GO:0021615 IEA
    trigeminal nerve structural organization GO:0021637 ISS
    vagus nerve morphogenesis GO:0021644 IEA
    postganglionic parasympathetic fiber development GO:0021784 IEA
    branchiomotor neuron axon guidance GO:0021785 ISS
    chemorepulsion of branchiomotor axon GO:0021793 IEA
    anterior commissure morphogenesis GO:0021960 IEA
    regulation of cell migration GO:0030334 IBA
    embryonic heart tube development GO:0035050 IEA
    sympathetic nervous system development GO:0048485 IEA
    regulation of axon extension involved in axon guidance GO:0048841 IEA
    regulation of negative chemotaxis GO:0050923 IEA
    semaphorin-plexin signaling pathway GO:0071526 IBA
    semaphorin-plexin signaling pathway GO:0071526 ISS
    semaphorin-plexin signaling pathway GO:0071526 NAS
    sympathetic neuron axon guidance GO:0097492 IEA
    maintenance of synapse structure GO:0099558 IEA
Subcellular Localization
    semaphorin receptor complex GO:0002116 IBA
    semaphorin receptor complex GO:0002116 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    cerebellar climbing fiber to Purkinje cell synapse GO:0150053 IEA
 Experiment description of studies that identified PLXNA4 in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLXNA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 HNRNPCL2 440563
Cross-Linking-MS (XL-MS) Homo sapiens
3 HNRNPCL3 649330
Cross-Linking-MS (XL-MS) Homo sapiens
4 TOMM22 56993
Co-fractionation Homo sapiens
5 GPM6A 2823
Affinity Capture-MS Homo sapiens
6 SGCZ  
Affinity Capture-MS Homo sapiens
7 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NTRK3 4916
Affinity Capture-Western Homo sapiens
9 CRYZL1 9946
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HNRNPCL1 343069
Cross-Linking-MS (XL-MS) Homo sapiens
11 NTRK1 4914
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 DCT 1638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MTX1 4580
Co-fractionation Homo sapiens
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