Gene description for RNF149
Gene name ring finger protein 149
Gene symbol RNF149
Other names/aliases DNAPTP2
Species Homo sapiens
 Database cross references - RNF149
ExoCarta ExoCarta_284996
Vesiclepedia VP_284996
Entrez Gene 284996
HGNC 23137
UniProt Q8NC42  
 RNF149 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RNF149
Molecular Function
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    protein ubiquitination GO:0016567 IEA
    regulation of protein stability GO:0031647 IEA
    negative regulation of MAPK cascade GO:0043409 IEA
    cellular response to xenobiotic stimulus GO:0071466 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    late endosome GO:0005770 IBA
    endoplasmic reticulum GO:0005783 IBA
    Golgi apparatus GO:0005794 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified RNF149 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RNF149
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
2 PTPRF 5792
Affinity Capture-MS Homo sapiens
3 EMC4 51234
Affinity Capture-MS Homo sapiens
4 HFE  
Affinity Capture-MS Homo sapiens
5 SGCA  
Affinity Capture-MS Homo sapiens
6 GJD3  
Proximity Label-MS Homo sapiens
7 NOTCH3 4854
Affinity Capture-MS Homo sapiens
8 SNX3 8724
Affinity Capture-MS Homo sapiens
9 LGR4 55366
Affinity Capture-MS Homo sapiens
10 MFAP3  
Affinity Capture-MS Homo sapiens
11 LITAF 9516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ZFAND2B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
13 NRSN1  
Affinity Capture-MS Homo sapiens
14 GPM6A 2823
Affinity Capture-MS Homo sapiens
15 CCDC91 55297
Affinity Capture-MS Homo sapiens
16 CSNK1G2 1455
Affinity Capture-MS Homo sapiens
17 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ATP12A 479
Affinity Capture-MS Homo sapiens
19 BNIP3L  
Affinity Capture-MS Homo sapiens
20 IFNGR1 3459
Affinity Capture-MS Homo sapiens
21 RET 5979
Affinity Capture-MS Homo sapiens
22 BMPR1A 657
Affinity Capture-MS Homo sapiens
23 PRPF40A 55660
Affinity Capture-MS Homo sapiens
24 LTN1 26046
Affinity Capture-MS Homo sapiens
25 SLC12A2 6558
Affinity Capture-MS Homo sapiens
26 LRP10 26020
Affinity Capture-MS Homo sapiens
27 HEPACAM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TMEM231 79583
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ABCA3 21
Affinity Capture-MS Homo sapiens
30 PIGN 23556
Affinity Capture-MS Homo sapiens
31 SLC20A1 6574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
33 CTLA4  
Affinity Capture-MS Homo sapiens
34 TMEM183A  
Affinity Capture-MS Homo sapiens
35 ADCY6 112
Affinity Capture-MS Homo sapiens
36 AKIRIN2  
Affinity Capture-MS Homo sapiens
37 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NR2F2  
Affinity Capture-MS Homo sapiens
39 UNC5B 219699
Affinity Capture-MS Homo sapiens
40 TMEM30A 55754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RNF133  
Affinity Capture-MS Homo sapiens
42 CD3E 916
Affinity Capture-MS Homo sapiens
43 NBR1 4077
Affinity Capture-MS Homo sapiens
44 TOM1 10043
Affinity Capture-MS Homo sapiens
45 ACVR2B  
Affinity Capture-MS Homo sapiens
46 UPK3BL 100134938
Affinity Capture-MS Homo sapiens
47 BTNL8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 STX6 10228
Affinity Capture-MS Homo sapiens
49 TMEM192 201931
Affinity Capture-MS Homo sapiens
50 TPCN2  
Affinity Capture-MS Homo sapiens
51 EDAR  
Affinity Capture-MS Homo sapiens
52 STXBP3 6814
Affinity Capture-MS Homo sapiens
53 VCAM1 7412
Affinity Capture-Western Homo sapiens
54 USP2  
Biochemical Activity Homo sapiens
55 SDCBP 6386
Affinity Capture-MS Homo sapiens
56 COX1 4512
Affinity Capture-MS Homo sapiens
57 FGFR1 2260
Affinity Capture-MS Homo sapiens
58 ACVR1 90
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 STX12 23673
Affinity Capture-MS Homo sapiens
60 CLDND1 56650
Affinity Capture-MS Homo sapiens
61 NOTCH2 4853
Affinity Capture-MS Homo sapiens
62 EMC1 23065
Affinity Capture-MS Homo sapiens
63 NDFIP1 80762
Affinity Capture-MS Homo sapiens
64 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CNNM1  
Affinity Capture-MS Homo sapiens
66 NOMO1 23420
Affinity Capture-MS Homo sapiens
67 TFB2M  
Affinity Capture-MS Homo sapiens
68 IGF1R 3480
Affinity Capture-Western Homo sapiens
69 TRAM1 23471
Affinity Capture-MS Homo sapiens
70 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SEC62 7095
Affinity Capture-MS Homo sapiens
72 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ZDHHC12  
Affinity Capture-MS Homo sapiens
74 BROX 148362
Affinity Capture-MS Homo sapiens
75 BAG6 7917
Affinity Capture-Western Homo sapiens
76 SLC20A2 6575
Affinity Capture-MS Homo sapiens
77 USP8 9101
Affinity Capture-MS Homo sapiens
78 ACVR1B 91
Affinity Capture-MS Homo sapiens
79 FYN 2534
Affinity Capture-MS Homo sapiens
80 C16orf58 64755
Affinity Capture-MS Homo sapiens
81 CCDC85C  
Affinity Capture-MS Homo sapiens
82 ARL17A  
Affinity Capture-MS Homo sapiens
83 ARRDC3 57561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 CSNK1E 1454
Affinity Capture-MS Homo sapiens
85 NARS2  
Affinity Capture-MS Homo sapiens
86 RGS9BP  
Affinity Capture-MS Homo sapiens
87 BRAF  
Affinity Capture-Western Homo sapiens
88 ATP2B4 493
Affinity Capture-MS Homo sapiens
89 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
91 EPHB4 2050
Affinity Capture-MS Homo sapiens
92 VCP 7415
Affinity Capture-Western Homo sapiens
93 PCDHA8  
Affinity Capture-MS Homo sapiens
94 SEC63 11231
Affinity Capture-MS Homo sapiens
95 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
96 ACVR2A  
Affinity Capture-MS Homo sapiens
97 PCDHB7  
Affinity Capture-MS Homo sapiens
98 HUS1  
Affinity Capture-MS Homo sapiens
99 EMC3 55831
Affinity Capture-MS Homo sapiens
100 TTYH1  
Affinity Capture-MS Homo sapiens
101 CANX 821
Affinity Capture-MS Homo sapiens
102 NRSN2  
Affinity Capture-MS Homo sapiens
103 EMC8 10328
Affinity Capture-MS Homo sapiens
104 MARCH1  
Affinity Capture-MS Homo sapiens
105 NDFIP2 54602
Affinity Capture-MS Homo sapiens
106 DUOXA2  
Affinity Capture-MS Homo sapiens
107 SMIM5  
Affinity Capture-MS Homo sapiens
108 ADCY9 115
Affinity Capture-MS Homo sapiens
109 TBC1D22B  
Affinity Capture-MS Homo sapiens
110 RNF128  
Affinity Capture-MS Homo sapiens
111 EMC10 284361
Affinity Capture-MS Homo sapiens
112 PTPRA 5786
Affinity Capture-MS Homo sapiens
113 CERK 64781
Affinity Capture-MS Homo sapiens
114 VAMP3 9341
Affinity Capture-MS Homo sapiens
115 RNF19A  
Affinity Capture-MS Homo sapiens
116 TMEM74  
Affinity Capture-MS Homo sapiens
117 CSNK1G1 53944
Affinity Capture-MS Homo sapiens
118 KCNE3  
Affinity Capture-MS Homo sapiens
119 FGFR2 2263
Affinity Capture-MS Homo sapiens
120 LSMEM2  
Affinity Capture-MS Homo sapiens
121 RAB2B 84932
Affinity Capture-MS Homo sapiens
122 JAK1 3716
Affinity Capture-MS Homo sapiens
123 RELT  
Affinity Capture-MS Homo sapiens
124 EMC2 9694
Affinity Capture-MS Homo sapiens
125 BNIP3 664
Affinity Capture-MS Homo sapiens
126 PRRG3  
Affinity Capture-MS Homo sapiens
127 EMC6 83460
Affinity Capture-MS Homo sapiens
128 CRLF2 64109
Affinity Capture-MS Homo sapiens
129 PLXNB2 23654
Affinity Capture-MS Homo sapiens
130 DGAT1 8694
Affinity Capture-MS Homo sapiens
131 IL17RB  
Affinity Capture-MS Homo sapiens
132 GRK6 2870
Affinity Capture-MS Homo sapiens
133 TGFBR2 7048
Affinity Capture-MS Homo sapiens
134 RBM25 58517
Affinity Capture-MS Homo sapiens
135 GOSR1 9527
Affinity Capture-MS Homo sapiens
136 LSR 51599
Affinity Capture-MS Homo sapiens
137 ABCD4 5826
Affinity Capture-MS Homo sapiens
138 RNF19B  
Affinity Capture-MS Homo sapiens
139 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
140 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 LMAN2L 81562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 CDC6  
Affinity Capture-MS Homo sapiens
143 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
144 DLK1  
Affinity Capture-MS Homo sapiens
145 UBE2D2 7322
Reconstituted Complex Homo sapiens
146 TUBB1 81027
Affinity Capture-MS Homo sapiens
147 STARD3 10948
Affinity Capture-MS Homo sapiens
148 SFTPC  
Affinity Capture-MS Homo sapiens
149 NAMPT 10135
Affinity Capture-MS Homo sapiens
150 PPP1R13B  
Affinity Capture-MS Homo sapiens
151 RAB2A 5862
Affinity Capture-MS Homo sapiens
152 PLXNA4 91584
Affinity Capture-MS Homo sapiens
153 PTPRS 5802
Affinity Capture-MS Homo sapiens
154 GOLGA5 9950
Affinity Capture-MS Homo sapiens
155 HSDL1 83693
Affinity Capture-MS Homo sapiens
156 BMPR2 659
Affinity Capture-MS Homo sapiens
157 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 SEC61A1 29927
Affinity Capture-MS Homo sapiens
159 ANKRD46 157567
Affinity Capture-MS Homo sapiens
160 ADIPOR1 51094
Affinity Capture-MS Homo sapiens
161 ZDHHC13 54503
Affinity Capture-MS Homo sapiens
162 PVRL3 25945
Affinity Capture-MS Homo sapiens
163 CC2D1A 54862
Affinity Capture-MS Homo sapiens
164 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RNF149 is involved
No pathways found





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