Gene description for TNFRSF10B
Gene name tumor necrosis factor receptor superfamily, member 10b
Gene symbol TNFRSF10B
Other names/aliases CD262
DR5
KILLER
KILLER/DR5
TRAIL-R2
TRAILR2
TRICK2
TRICK2A
TRICK2B
TRICKB
ZTNFR9
Species Homo sapiens
 Database cross references - TNFRSF10B
ExoCarta ExoCarta_8795
Vesiclepedia VP_8795
Entrez Gene 8795
HGNC 11905
MIM 603612
UniProt O14763  
 TNFRSF10B identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for TNFRSF10B
Molecular Function
    protein binding GO:0005515 IPI
    TRAIL receptor activity GO:0036463 IDA
    signaling receptor activity GO:0038023 NAS
    TRAIL binding GO:0045569 NAS
Biological Process
    defense response to tumor cell GO:0002357 IDA
    apoptotic process GO:0006915 NAS
    cell surface receptor signaling pathway GO:0007166 TAS
    activation of NF-kappaB-inducing kinase activity GO:0007250 NAS
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 NAS
    response to endoplasmic reticulum stress GO:0034976 IDA
    TRAIL-activated apoptotic signaling pathway GO:0036462 IBA
    TRAIL-activated apoptotic signaling pathway GO:0036462 IMP
    regulation of apoptotic process GO:0042981 NAS
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEP
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IMP
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
 Experiment description of studies that identified TNFRSF10B in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for TNFRSF10B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
3 GPM6A 2823
Affinity Capture-MS Homo sapiens
4 LRRIQ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SKP1 6500
Affinity Capture-Western Homo sapiens
6 MFAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SLC15A4 121260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TNFSF10 8743
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 IL20RB  
Affinity Capture-MS Homo sapiens
10 CSNK1G2 1455
Affinity Capture-MS Homo sapiens
11 TRIM66  
Affinity Capture-MS Homo sapiens
12 MARCH2  
Affinity Capture-MS Homo sapiens
13 CYB561D2 11068
Affinity Capture-MS Homo sapiens
14 IFNGR1 3459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UGCG 7357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RIPK1 8737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 FFAR1  
Affinity Capture-MS Homo sapiens
18 HEPACAM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ARHGDIA 396
Co-purification Homo sapiens
20 PTRH2 51651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SLC20A1 6574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ASIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TMEM231 79583
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MUC1 4582
Two-hybrid Homo sapiens
26 GPRC5B 51704
Affinity Capture-MS Homo sapiens
27 SLAMF1 6504
Affinity Capture-MS Homo sapiens
28 RHOA 387
Co-purification Homo sapiens
29 ACVR2B  
Affinity Capture-MS Homo sapiens
30 EDAR  
Affinity Capture-MS Homo sapiens
31 TNFRSF13B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MCAM 4162
Proximity Label-MS Homo sapiens
33 CUL1 8454
Affinity Capture-Western Homo sapiens
34 TIMM50 92609
Two-hybrid Homo sapiens
35 FGFR1 2260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 TMEM59 9528
Affinity Capture-MS Homo sapiens
38 STX12 23673
Affinity Capture-MS Homo sapiens
39 CLDND1 56650
Affinity Capture-MS Homo sapiens
40 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
41 DDX58 23586
Affinity Capture-RNA Homo sapiens
42 PRPH2 5961
Affinity Capture-MS Homo sapiens
43 MICA 100507436
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 VSIG4  
Affinity Capture-MS Homo sapiens
46 NDFIP1 80762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MTUS2 23281
Affinity Capture-MS Homo sapiens
48 FASLG 356
Co-purification Homo sapiens
49 PTPRN2  
Affinity Capture-MS Homo sapiens
50 RBX1 9978
Affinity Capture-Western Homo sapiens
51 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 ACVR1 90
Affinity Capture-MS Homo sapiens
53 CBL 867
Affinity Capture-Western Homo sapiens
54 GPR35  
Affinity Capture-MS Homo sapiens
55 SCAMP1 9522
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 DNAJC5 80331
Proximity Label-MS Homo sapiens
57 FYN 2534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RNF128  
Affinity Capture-MS Homo sapiens
59 ARRDC3 57561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TNFRSF10C  
Affinity Capture-MS Homo sapiens
61 MAPK8 5599
Co-purification Homo sapiens
62 ITCH 83737
Affinity Capture-Western Homo sapiens
63 PROKR1  
Affinity Capture-MS Homo sapiens
64 CSPG5 10675
Affinity Capture-MS Homo sapiens
65 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
66 SYT11 23208
Affinity Capture-MS Homo sapiens
67 REEP5 7905
Affinity Capture-MS Homo sapiens
68 MARCH3 115123
Affinity Capture-MS Homo sapiens
69 LPPR1  
Affinity Capture-MS Homo sapiens
70 LAPTM4B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 CXCR4 7852
Affinity Capture-MS Homo sapiens
72 SKP2  
Affinity Capture-Western Homo sapiens
73 MARCH1  
Affinity Capture-MS Homo sapiens
74 CD83  
Affinity Capture-MS Homo sapiens
75 PCDHAC1  
Affinity Capture-MS Homo sapiens
76 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 LAMP1 3916
Proximity Label-MS Homo sapiens
78 MARCH8  
Affinity Capture-MS Homo sapiens
79 Bmpr1a  
Affinity Capture-MS Mus musculus
80 TNFRSF1A 7132
Co-purification Homo sapiens
81 VAMP3 9341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 CUL3 8452
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
83 EGFR 1956
Affinity Capture-MS Homo sapiens
84 OPRL1 4987
Affinity Capture-MS Homo sapiens
85 LPAR1 1902
Affinity Capture-MS Homo sapiens
86 KCNE3  
Affinity Capture-MS Homo sapiens
87 MICB  
Affinity Capture-MS Homo sapiens
88 IFNAR1  
Affinity Capture-MS Homo sapiens
89 RPA3 6119
Proximity Label-MS Homo sapiens
90 RELT  
Affinity Capture-MS Homo sapiens
91 CASP10  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
92 ASPH 444
Two-hybrid Homo sapiens
93 MSN 4478
Co-purification Homo sapiens
94 ZDHHC15 158866
Two-hybrid Homo sapiens
95 AVPR2  
Affinity Capture-MS Homo sapiens
96 FLVCR1 28982
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 FADD 8772
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
98 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
99 RNF11 26994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 TGFBR2 7048
Affinity Capture-MS Homo sapiens
101 PRAF2 11230
Affinity Capture-MS Homo sapiens
102 STX3 6809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 TMEM214 54867
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 IL1R1 3554
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 LSR 51599
Affinity Capture-MS Homo sapiens
106 FAS 355
Co-purification Homo sapiens
107 EZR 7430
Co-purification Homo sapiens
108 RYK 6259
Affinity Capture-MS Homo sapiens
109 CASP8 841
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
110 BID  
Co-purification Homo sapiens
111 ANKRD26P1  
Affinity Capture-MS Homo sapiens
112 GPR3  
Affinity Capture-MS Homo sapiens
113 GPRC5D  
Affinity Capture-MS Homo sapiens
114 DNAJC5B  
Proximity Label-MS Homo sapiens
115 SPPL2B 56928
Affinity Capture-MS Homo sapiens
116 DDR1 780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 VAMP5 10791
Affinity Capture-MS Homo sapiens
118 KIR2DL4  
Affinity Capture-MS Homo sapiens
119 KRAS 3845
Proximity Label-MS Homo sapiens
120 TRADD 8717
Affinity Capture-Western Homo sapiens
121 TMEM31  
Two-hybrid Homo sapiens
122 CKAP4 10970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 SLC1A1 6505
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 CAV1 857
Co-fractionation Homo sapiens
125 KCNE4  
Affinity Capture-MS Homo sapiens
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