Gene description for MICA
Gene name MHC class I polypeptide-related sequence A
Gene symbol MICA
Other names/aliases MIC-A
PERB11.1
Species Homo sapiens
 Database cross references - MICA
ExoCarta ExoCarta_100507436
Vesiclepedia VP_100507436
Entrez Gene 100507436
HGNC 7090
MIM 600169
UniProt Q29983  
 MICA identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Trophoblast cells 21276792    
 Gene ontology annotations for MICA
Molecular Function
    protein binding GO:0005515 IPI
    beta-2-microglobulin binding GO:0030881 IDA
    natural killer cell lectin-like receptor binding GO:0046703 IDA
    natural killer cell lectin-like receptor binding GO:0046703 IPI
    receptor ligand activity GO:0048018 IDA
Biological Process
    T cell mediated cytotoxicity GO:0001913 IDA
    immune response to tumor cell GO:0002418 IDA
    immune response GO:0006955 IBA
    DNA damage response GO:0006974 IDA
    signal transduction GO:0007165 IEA
    response to heat GO:0009408 IDA
    killing of cells of another organism GO:0031640 IEA
    negative regulation of natural killer cell activation GO:0032815 IDA
    natural killer cell mediated cytotoxicity GO:0042267 IDA
    defense response to bacterium GO:0042742 IDA
    negative regulation of natural killer cell mediated cytotoxicity GO:0045953 IMP
    gamma-delta T cell activation GO:0046629 IDA
    defense response to virus GO:0051607 IDA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 IDA
 Experiment description of studies that identified MICA in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 121
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|HSP60|HSP70|CD81
Enriched markers
✔
HSPA5
Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21276792    
Organism Homo sapiens
Experiment description Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells.
Authors "Atay S, Gercel-Taylor C, Kesimer M, Taylor DD."
Journal name ECR
Publication year 2011
Sample Trophoblast cells
Sample name Sw71
Isolation/purification methods Differential centrifugation
Ultrafiltration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for MICA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FLVCR1 28982
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PHF7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 POT1  
Two-hybrid Homo sapiens
4 GPIHBP1  
Affinity Capture-MS Homo sapiens
5 RAB35 11021
Affinity Capture-MS Homo sapiens
6 ELSPBP1  
Affinity Capture-MS Homo sapiens
7 IL13RA2 3598
Affinity Capture-MS Homo sapiens
8 NUP160 23279
Affinity Capture-MS Homo sapiens
9 AP1M1 8907
Affinity Capture-MS Homo sapiens
10 ATL3 25923
Affinity Capture-MS Homo sapiens
11 ORC4  
Affinity Capture-MS Homo sapiens
12 RETNLB  
Affinity Capture-MS Homo sapiens
13 Tpm1 22003
Affinity Capture-MS Mus musculus
14 HMOX2 3163
Affinity Capture-MS Homo sapiens
15 DEFA1 1667
Affinity Capture-MS Homo sapiens
16 TCTN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 KIF14 9928
Affinity Capture-MS Homo sapiens
18 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 DEFB109P1B  
Affinity Capture-MS Homo sapiens
20 PCDH1 5097
Affinity Capture-MS Homo sapiens
21 RAB13 5872
Affinity Capture-MS Homo sapiens
22 MSMO1 6307
Affinity Capture-MS Homo sapiens
23 SGCA  
Affinity Capture-MS Homo sapiens
24 TNFSF8  
Affinity Capture-MS Homo sapiens
25 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HPN  
Affinity Capture-MS Homo sapiens
27 PSCA 8000
Affinity Capture-MS Homo sapiens
28 GGH 8836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 Chmp4b 75608
Affinity Capture-MS Mus musculus
30 RYK 6259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 VAMP3 9341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ERLEC1 27248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SLC39A11  
Affinity Capture-MS Homo sapiens
34 RPS6KA4 8986
Affinity Capture-MS Homo sapiens
35 LRFN1  
Affinity Capture-MS Homo sapiens
36 TNPO3 23534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 VAC14 55697
Affinity Capture-MS Homo sapiens
38 F3 2152
Affinity Capture-MS Homo sapiens
39 AAR2 25980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SPPL2B 56928
Affinity Capture-MS Homo sapiens
41 SCAMP3 10067
Affinity Capture-MS Homo sapiens
42 SLURP1  
Affinity Capture-MS Homo sapiens
43 CLDND1 56650
Affinity Capture-MS Homo sapiens
44 SPINK4  
Affinity Capture-MS Homo sapiens
45 INSL5  
Affinity Capture-MS Homo sapiens
46 MTOR 2475
Affinity Capture-MS Homo sapiens
47 MFAP5  
Affinity Capture-MS Homo sapiens
48 MYADM 91663
Affinity Capture-MS Homo sapiens
49 EPHA7 2045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SCAMP1 9522
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 DEFB136  
Affinity Capture-MS Homo sapiens
52 S1PR4  
Affinity Capture-MS Homo sapiens
53 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 FAM19A3  
Affinity Capture-MS Homo sapiens
55 DEFB107A  
Affinity Capture-MS Homo sapiens
56 MICB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SPATA31D3  
Affinity Capture-MS Homo sapiens
58 DLK1  
Affinity Capture-MS Homo sapiens
59 IL2RA  
Affinity Capture-MS Homo sapiens
60 AVP  
Affinity Capture-MS Homo sapiens
61 LCN9  
Affinity Capture-MS Homo sapiens
62 WFDC3  
Affinity Capture-MS Homo sapiens
63 SEZ6L2 26470
Affinity Capture-MS Homo sapiens
64 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
65 DEFA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ADAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 DEFB135  
Affinity Capture-MS Homo sapiens
68 TMEM87A 25963
Affinity Capture-MS Homo sapiens
69 IL27RA 9466
Affinity Capture-MS Homo sapiens
70 PRSS8 5652
Affinity Capture-MS Homo sapiens
71 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 VIPR1  
Affinity Capture-MS Homo sapiens
73 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
74 FAM19A2 338811
Affinity Capture-MS Homo sapiens
75 TNPO2 30000
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PGLYRP3 114771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 PGRMC2 10424
Affinity Capture-MS Homo sapiens
79 EDDM3B  
Affinity Capture-MS Homo sapiens
80 ITM2B 9445
Affinity Capture-MS Homo sapiens
81 TNFAIP2 7127
Affinity Capture-MS Homo sapiens
82 IGFL3  
Affinity Capture-MS Homo sapiens
83 TNFSF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 VAMP5 10791
Affinity Capture-MS Homo sapiens
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