Gene description for PVRL3
Gene name poliovirus receptor-related 3
Gene symbol PVRL3
Other names/aliases CD113
CDW113
NECTIN-3
PPR3
PRR3
PVRR3
Species Homo sapiens
 Database cross references - PVRL3
ExoCarta ExoCarta_25945
Vesiclepedia VP_25945
Entrez Gene 25945
HGNC 17664
MIM 607147
UniProt Q9NQS3  
 PVRL3 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PVRL3
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 ISS
    cell adhesion molecule binding GO:0050839 IPI
    cell adhesion mediator activity GO:0098631 ISS
Biological Process
    lens morphogenesis in camera-type eye GO:0002089 IEA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 ISS
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IBA
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 ISS
    fertilization GO:0009566 IEA
    symbiont entry into host cell GO:0046718 IEA
    retina morphogenesis in camera-type eye GO:0060042 IEA
    establishment of protein localization to plasma membrane GO:0061951 ISS
    cochlea morphogenesis GO:0090103 ISS
    regulation of postsynapse assembly GO:0150052 IEA
    protein localization to cell junction GO:1902414 IEA
Subcellular Localization
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 ISS
    adherens junction GO:0005912 IBA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
    apical junction complex GO:0043296 IBA
    cell-cell contact zone GO:0044291 IEA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
    postsynaptic density membrane GO:0098839 IEA
 Experiment description of studies that identified PVRL3 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PVRL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 KRTAP10-7  
Two-hybrid Homo sapiens
3 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
4 HFE  
Affinity Capture-MS Homo sapiens
5 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 Esrrb  
Affinity Capture-MS Mus musculus
7 PICK1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
8 NPTX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 BTNL2  
Affinity Capture-MS Homo sapiens
10 TMEM106A  
Affinity Capture-MS Homo sapiens
11 CYSRT1 375791
Two-hybrid Homo sapiens
12 KRTAP1-3  
Two-hybrid Homo sapiens
13 Spast  
Affinity Capture-MS Mus musculus
14 FBXO7 25793
Affinity Capture-MS Homo sapiens
15 MAK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PDK1 5163
Affinity Capture-MS Homo sapiens
17 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 KRTAP4-1  
Two-hybrid Homo sapiens
19 NBPF19  
Two-hybrid Homo sapiens
20 KRTAP9-3  
Two-hybrid Homo sapiens
21 LGALS1 3956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SDF2 6388
Affinity Capture-MS Homo sapiens
23 CLEC4A  
Affinity Capture-MS Homo sapiens
24 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 KRTAP5-9  
Two-hybrid Homo sapiens
26 Ncapg2  
Affinity Capture-MS Mus musculus
27 KRTAP1-1  
Two-hybrid Homo sapiens
28 KRTAP10-3  
Two-hybrid Homo sapiens
29 KRTAP5-11  
Two-hybrid Homo sapiens
30 SMEK1 55671
Affinity Capture-MS Homo sapiens
31 OTX1  
Two-hybrid Homo sapiens
32 MCAM 4162
Proximity Label-MS Homo sapiens
33 PVRL2 5819
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 INPP5K 51763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SDF2L1 23753
Affinity Capture-MS Homo sapiens
36 PVRL1 5818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CHMP4B 128866
Affinity Capture-MS Homo sapiens
38 MLLT4 4301
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
39 PARD3 56288
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 NLRP3  
Affinity Capture-MS Homo sapiens
41 SPPL2B 56928
Affinity Capture-MS Homo sapiens
42 ASPH 444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 FBXW7  
Synthetic Lethality Homo sapiens
44 PVR 5817
Reconstituted Complex Homo sapiens
45 ERO1LB 56605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 KRTAP4-2  
Two-hybrid Homo sapiens
47 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 Mad2l1 56150
Affinity Capture-MS Mus musculus
49 Cul4a  
Affinity Capture-MS Mus musculus
50 PVRL3 25945
Co-purification Homo sapiens
Co-purification Homo sapiens
51 Tpx2  
Affinity Capture-MS Mus musculus
52 RGS20 8601
Two-hybrid Homo sapiens
53 PCDHB3  
Affinity Capture-MS Homo sapiens
54 COL14A1 7373
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 KRTAP10-9  
Two-hybrid Homo sapiens
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