Gene description for EMC3
Gene name ER membrane protein complex subunit 3
Gene symbol EMC3
Other names/aliases POB
TMEM111
Species Homo sapiens
 Database cross references - EMC3
ExoCarta ExoCarta_55831
Vesiclepedia VP_55831
Entrez Gene 55831
HGNC 23999
UniProt Q9P0I2  
 EMC3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for EMC3
Molecular Function
    protein binding GO:0005515 IPI
    membrane insertase activity GO:0032977 IMP
Biological Process
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 IDA
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 IMP
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    membrane GO:0016020 IDA
    EMC complex GO:0072546 IBA
    EMC complex GO:0072546 IDA
    EMC complex GO:0072546 IPI
    EMC complex GO:0072546 ISS
 Experiment description of studies that identified EMC3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for EMC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 TMCO1 54499
Affinity Capture-MS Homo sapiens
4 EMC6 83460
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 C7orf34  
Affinity Capture-MS Homo sapiens
6 SAA1 6288
Affinity Capture-MS Homo sapiens
7 SEC63 11231
Proximity Label-MS Homo sapiens
8 SLC17A2  
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
11 Emc1 230866
Affinity Capture-MS Mus musculus
12 CYB5B 80777
Co-fractionation Homo sapiens
13 DNAJC25 548645
Proximity Label-MS Homo sapiens
14 SLC18A1  
Affinity Capture-MS Homo sapiens
15 EMC10 284361
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DERL2 51009
Affinity Capture-MS Homo sapiens
17 RHOT2 89941
Proximity Label-MS Homo sapiens
18 CANX 821
Affinity Capture-MS Homo sapiens
19 VAPA 9218
Affinity Capture-MS Homo sapiens
20 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 EMC8 10328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 EMC1 23065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 EMC9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CLPP 8192
Proximity Label-MS Homo sapiens
25 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 EMC7 56851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 Emc6  
Affinity Capture-MS Mus musculus
28 TPTE  
Proximity Label-MS Homo sapiens
29 CTDNEP1 23399
Proximity Label-MS Homo sapiens
30 EMC4 51234
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ATP1B4  
Affinity Capture-MS Homo sapiens
32 UBC 7316
Affinity Capture-MS Homo sapiens
33 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
34 FKBP8 23770
Proximity Label-MS Homo sapiens
35 RB1CC1 9821
Affinity Capture-MS Homo sapiens
36 FBXO6 26270
Affinity Capture-MS Homo sapiens
37 PTPN1 5770
Proximity Label-MS Homo sapiens
38 COPE 11316
Affinity Capture-MS Homo sapiens
39 PDIA4 9601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 UBQLN2 29978
Affinity Capture-MS Homo sapiens
41 CPT1A 1374
Co-fractionation Homo sapiens
42 DEFB106A  
Affinity Capture-MS Homo sapiens
43 MMGT1 93380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SCN4A  
Affinity Capture-MS Homo sapiens
45 NDUFB6 4712
Affinity Capture-MS Homo sapiens
46 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
47 UBAC2 337867
Affinity Capture-MS Homo sapiens
48 FIS1 51024
Proximity Label-MS Homo sapiens
49 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
50 SDF2L1 23753
Affinity Capture-MS Homo sapiens
51 FBXO2 26232
Affinity Capture-MS Homo sapiens
52 EMC2 9694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RAB7A 7879
Proximity Label-MS Homo sapiens
54 SLC4A8 9498
Affinity Capture-MS Homo sapiens
55 SLC28A2  
Affinity Capture-MS Homo sapiens
56 RPN1 6184
Affinity Capture-MS Homo sapiens
57 TRDN 10345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DNAJC16  
Proximity Label-MS Homo sapiens
59 DNAJC1 64215
Proximity Label-MS Homo sapiens
60 GNRH1  
Affinity Capture-MS Homo sapiens
61 MAK16  
Affinity Capture-MS Homo sapiens
62 C9orf72  
Affinity Capture-MS Homo sapiens
63 FAM19A2 338811
Affinity Capture-MS Homo sapiens
64 CCDC47 57003
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 APOA2 336
Affinity Capture-MS Homo sapiens
66 DDOST 1650
Affinity Capture-MS Homo sapiens
67 TMEM33 55161
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 IFNA21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 DNAJC22  
Proximity Label-MS Homo sapiens
71 PLAC9 219348
Affinity Capture-MS Homo sapiens
72 SEC61B 10952
Affinity Capture-MS Homo sapiens
73 CCDC8  
Affinity Capture-MS Homo sapiens
74 RMDN3 55177
Proximity Label-MS Homo sapiens
75 SLC25A46 91137
Proximity Label-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here