Gene description for FIG4
Gene name FIG4 phosphoinositide 5-phosphatase
Gene symbol FIG4
Other names/aliases ALS11
BTOP
CMT4J
KIAA0274
SAC3
YVS
dJ249I4.1
Species Homo sapiens
 Database cross references - FIG4
ExoCarta ExoCarta_9896
Vesiclepedia VP_9896
Entrez Gene 9896
HGNC 16873
MIM 609390
UniProt Q92562  
 FIG4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FIG4
Molecular Function
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 IEA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IEA
    protein serine/threonine phosphatase activity GO:0004722 IEA
    protein binding GO:0005515 IPI
    phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity GO:0034485 IEA
    phosphatidylinositol-4-phosphate phosphatase activity GO:0043812 IEA
    phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity GO:0043813 IBA
    phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity GO:0043813 TAS
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    vacuole organization GO:0007033 IEA
    locomotory behavior GO:0007626 IEA
    positive regulation of neuron projection development GO:0010976 IEA
    negative regulation of myelination GO:0031642 IEA
    myelin assembly GO:0032288 IEA
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IEA
    pigmentation GO:0043473 IEA
    phosphatidylinositol dephosphorylation GO:0046856 IBA
    neuron development GO:0048666 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    lipid droplet GO:0005811 IDA
    endosome membrane GO:0010008 IDA
    early endosome membrane GO:0031901 TAS
    late endosome membrane GO:0031902 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome GO:0055037 IEA
 Experiment description of studies that identified FIG4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FIG4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YEATS4  
Affinity Capture-MS Homo sapiens
2 SNX27 81609
Affinity Capture-MS Homo sapiens
3 ATG9A 79065
Proximity Label-MS Homo sapiens
4 VAC14 55697
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 NPTN 27020
Affinity Capture-MS Homo sapiens
6 MYO18A 399687
Affinity Capture-MS Homo sapiens
7 CMAS 55907
Affinity Capture-MS Homo sapiens
8 ANK1 286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 KIF20A 10112
Affinity Capture-MS Homo sapiens
10 CSPP1  
Affinity Capture-MS Homo sapiens
11 PARK2  
Affinity Capture-MS Homo sapiens
12 PSME1 5720
Affinity Capture-MS Homo sapiens
13 CAPZB 832
Affinity Capture-MS Homo sapiens
14 BRCA2 675
Affinity Capture-MS Homo sapiens
15 KIF23 9493
Affinity Capture-MS Homo sapiens
16 RAB7A 7879
Proximity Label-MS Homo sapiens
17 GORASP2 26003
Two-hybrid Homo sapiens
18 PIKFYVE 200576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
19 PTGES3 10728
Affinity Capture-MS Homo sapiens
20 TWISTNB  
Affinity Capture-MS Homo sapiens
21 PTCH1  
Affinity Capture-MS Homo sapiens
22 CUL4A 8451
Affinity Capture-MS Homo sapiens
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