Gene description for ANK1
Gene name ankyrin 1, erythrocytic
Gene symbol ANK1
Other names/aliases ANK
SPH1
SPH2
Species Homo sapiens
 Database cross references - ANK1
ExoCarta ExoCarta_286
Vesiclepedia VP_286
Entrez Gene 286
HGNC 492
MIM 612641
UniProt P16157  
 ANK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ANK1
Molecular Function
    structural molecule activity GO:0005198 NAS
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    cytoskeletal anchor activity GO:0008093 IBA
    cytoskeletal anchor activity GO:0008093 IDA
    cytoskeletal anchor activity GO:0008093 TAS
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 TAS
    protein phosphatase binding GO:0019903 IPI
    spectrin binding GO:0030507 IBA
    spectrin binding GO:0030507 IDA
    spectrin binding GO:0030507 IPI
    spectrin binding GO:0030507 NAS
    transmembrane transporter binding GO:0044325 IBA
    ATPase binding GO:0051117 IPI
Biological Process
    exocytosis GO:0006887 NAS
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    cytoskeleton organization GO:0007010 NAS
    signal transduction GO:0007165 IEA
    positive regulation of organelle organization GO:0010638 IEA
    maintenance of epithelial cell apical/basal polarity GO:0045199 TAS
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 NAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    cytoplasmic side of plasma membrane GO:0009898 IDA
    spectrin-associated cytoskeleton GO:0014731 IDA
    basolateral plasma membrane GO:0016323 NAS
    sarcoplasmic reticulum GO:0016529 IEA
    Z disc GO:0030018 IEA
    axolemma GO:0030673 IEA
    M band GO:0031430 IEA
    sarcolemma GO:0042383 IEA
    neuron projection GO:0043005 IBA
    postsynaptic membrane GO:0045211 IEA
    ankyrin-1 complex GO:0170014 IDA
 Experiment description of studies that identified ANK1 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ANK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FIG4 9896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
3 SLC4A1 6521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 SLC4A3  
Affinity Capture-Western Homo sapiens
5 ANO6 196527
Affinity Capture-MS Homo sapiens
6 FGFR1 2260
Affinity Capture-MS Homo sapiens
7 RHAG  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
8 CUL3 8452
Affinity Capture-Western Homo sapiens
9 KCTD6  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 PDS5B 23047
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CHL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MEX3A  
Affinity Capture-RNA Homo sapiens
13 SPTB 6710
Reconstituted Complex Homo sapiens
14 SPTA1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
15 OBSCN 84033
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
16 HSPB1 3315
Two-hybrid Homo sapiens
17 FKBP8 23770
Affinity Capture-MS Homo sapiens
18 VAC14 55697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SPTBN1 6711
Affinity Capture-Western Homo sapiens
20 TGFBR1 7046
Affinity Capture-Western Homo sapiens
21 RYR1 6261
Reconstituted Complex Homo sapiens
22 TTN 7273
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
23 RAPGEF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PIKFYVE 200576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
26 CD44 960
Protein-peptide Homo sapiens
27 TIAM1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 NELFB 25920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 BRI3BP 140707
Affinity Capture-MS Homo sapiens
30 MB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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