Gene description for NELFB
Gene name negative elongation factor complex member B
Gene symbol NELFB
Other names/aliases COBRA1
NELF-B
Species Homo sapiens
 Database cross references - NELFB
ExoCarta ExoCarta_25920
Vesiclepedia VP_25920
Entrez Gene 25920
HGNC 24324
MIM 611180
UniProt Q8WX92  
 NELFB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for NELFB
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
Biological Process
    cell population proliferation GO:0008283 ISS
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 IBA
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 IDA
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 ISS
    stem cell differentiation GO:0048863 ISS
    negative regulation of stem cell differentiation GO:2000737 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    NELF complex GO:0032021 IBA
    NELF complex GO:0032021 IDA
    NELF complex GO:0032021 IPI
 Experiment description of studies that identified NELFB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NELFB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DENR 8562
Reconstituted Complex Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 KHDRBS3  
Reconstituted Complex Homo sapiens
4 NCBP1 4686
Affinity Capture-MS Homo sapiens
5 BRDT  
Affinity Capture-MS Homo sapiens
6 COIL  
Proximity Label-MS Homo sapiens
7 ELP6 54859
Co-fractionation Homo sapiens
8 EIF3C 8663
Co-fractionation Homo sapiens
9 KCTD10 83892
Affinity Capture-MS Homo sapiens
10 S100A6 6277
Affinity Capture-MS Homo sapiens
11 YWHAE 7531
Affinity Capture-MS Homo sapiens
12 EIF2B2 8892
Co-fractionation Homo sapiens
13 PRSS3 5646
Affinity Capture-MS Homo sapiens
14 BRCA1 672
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
15 ANK1 286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 C9orf78 51759
Affinity Capture-MS Homo sapiens
17 ILF2 3608
Co-fractionation Homo sapiens
18 INTS9 55756
Affinity Capture-MS Homo sapiens
19 DIMT1 27292
Co-fractionation Homo sapiens
20 IGF2BP3 10643
Co-fractionation Homo sapiens
21 Nelfa  
Affinity Capture-MS Mus musculus
22 POLR2I 5438
Affinity Capture-MS Homo sapiens
23 YWHAG 7532
Affinity Capture-MS Homo sapiens
24 XYLT1 64131
Affinity Capture-MS Homo sapiens
25 BRD4 23476
Affinity Capture-MS Homo sapiens
26 ATG16L1 55054
Affinity Capture-MS Homo sapiens
27 POLR2C 5432
Affinity Capture-MS Homo sapiens
28 NMT1 4836
Co-fractionation Homo sapiens
29 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 POLR2K  
Affinity Capture-MS Homo sapiens
31 EDC4 23644
Co-fractionation Homo sapiens
32 MRE11A 4361
Co-fractionation Homo sapiens
33 HCST  
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 SUPT5H 6829
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
36 CEBPE  
Two-hybrid Homo sapiens
37 NELFE 7936
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
38 NTRK1 4914
Affinity Capture-MS Homo sapiens
39 CENPM  
Affinity Capture-MS Homo sapiens
40 SYNCRIP 10492
Co-fractionation Homo sapiens
41 PPP2CA 5515
Affinity Capture-MS Homo sapiens
42 SRSF1 6426
Affinity Capture-MS Homo sapiens
43 RPN1 6184
Affinity Capture-MS Homo sapiens
44 RFC4 5984
Co-fractionation Homo sapiens
45 DDX1 1653
Co-fractionation Homo sapiens
46 DHX9 1660
Co-fractionation Homo sapiens
47 ABCF1 23
Co-fractionation Homo sapiens
48 CEBPA  
Protein-peptide Homo sapiens
49 Ncbp2  
Affinity Capture-MS Mus musculus
50 POLR2B 5431
Affinity Capture-MS Homo sapiens
51 TOMM40 10452
Co-fractionation Homo sapiens
52 POLR2H 5437
Affinity Capture-MS Homo sapiens
53 MSH2 4436
Affinity Capture-MS Homo sapiens
54 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 YBX1 4904
Co-fractionation Homo sapiens
56 NELFCD 51497
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 TPRX1  
Two-hybrid Homo sapiens
58 POLR2E 5434
Affinity Capture-MS Homo sapiens
59 CD274 29126
Affinity Capture-MS Homo sapiens
60 YWHAZ 7534
Affinity Capture-MS Homo sapiens
61 EIF2B3 8891
Co-fractionation Homo sapiens
62 POLR2A 5430
Affinity Capture-Western Homo sapiens
63 JUN 3725
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
64 MAPK6  
Two-hybrid Homo sapiens
65 FOS 2353
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
66 FABP5 2171
Affinity Capture-MS Homo sapiens
67 RPA3 6119
Proximity Label-MS Homo sapiens
68 CYP2J2  
Affinity Capture-MS Homo sapiens
69 CCT8 10694
Affinity Capture-MS Homo sapiens
70 SCO1  
Co-fractionation Homo sapiens
71 KIR2DL4  
Affinity Capture-MS Homo sapiens
72 FKBP3 2287
Co-fractionation Homo sapiens
73 HSPA9 3313
Reconstituted Complex Homo sapiens
74 NELFB 25920
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
75 PA2G4 5036
Co-fractionation Homo sapiens
76 GYPA  
Affinity Capture-MS Homo sapiens
77 ALDH3A2 224
Affinity Capture-MS Homo sapiens
78 POLR2F  
Affinity Capture-MS Homo sapiens
79 POLR2J  
Affinity Capture-MS Homo sapiens
80 PTBP1 5725
Co-fractionation Homo sapiens
81 NELFA  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
83 MGST1 4257
Reconstituted Complex Homo sapiens
84 YBX3 8531
Co-fractionation Homo sapiens
85 ATP1A1 476
Affinity Capture-MS Homo sapiens
86 RPL23A 6147
Co-fractionation Homo sapiens
87 KRAS 3845
Negative Genetic Homo sapiens
88 HNRNPU 3192
Co-fractionation Homo sapiens
89 SSRP1 6749
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NELFB is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat IEA Reactome
Disease IEA Reactome
Disease TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat TAS Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle IEA Reactome
HIV Life Cycle TAS Reactome
HIV Transcription Elongation IEA Reactome
HIV Transcription Elongation TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Late Phase of HIV Life Cycle IEA Reactome
Late Phase of HIV Life Cycle TAS Reactome
NTRK3 as a dependence receptor IEA Reactome
Pausing and recovery of HIV elongation IEA Reactome
Pausing and recovery of Tat-mediated HIV elongation IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
Signal Transduction IEA Reactome
Signaling by NTRK3 (TRKC) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
TP53 Regulates Transcription of DNA Repair Genes TAS Reactome
Transcription of the HIV genome IEA Reactome
Transcription of the HIV genome TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Infection Pathways TAS Reactome





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