ExoCarta: Gene summary

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Gene description for KLHL21
Gene name	kelch-like family member 21
Gene symbol	KLHL21
Other names/aliases	-
Species	Homo sapiens
Database cross references - KLHL21
ExoCarta	ExoCarta_9903
Vesiclepedia	VP_9903
Entrez Gene	9903
HGNC	29041
MIM	616262
UniProt	Q9UJP4
KLHL21 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells	36408942

Gene ontology annotations for KLHL21

Molecular Function
	ubiquitin-protein transferase activity	GO:0004842	IDA
	protein binding	GO:0005515	IPI
	cullin family protein binding	GO:0097602	IPI
	ubiquitin-like ligase-substrate adaptor activity	GO:1990756	IBA
Biological Process
	protein ubiquitination	GO:0016567	IDA
	protein ubiquitination	GO:0016567	IEA
	regulation of cytokinesis	GO:0032465	IMP
	chromosome passenger complex localization to spindle midzone	GO:0035853	IMP
	proteasome-mediated ubiquitin-dependent protein catabolic process	GO:0043161	IBA
	cell division	GO:0051301	IEA
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	polar microtubule	GO:0005827	IDA
	cytosol	GO:0005829	TAS
	Cul3-RING ubiquitin ligase complex	GO:0031463	IBA
	Cul3-RING ubiquitin ligase complex	GO:0031463	IDA

Experiment description of studies that identified KLHL21 in sEVs

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for KLHL21

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

KLHL9

55958

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

ZBTB34

403341

Affinity Capture-MS

Homo sapiens

ZNF414

Affinity Capture-MS

Homo sapiens

KLHL28

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

XPA

Affinity Capture-MS

Homo sapiens

GPR17

2840

Affinity Capture-MS

Homo sapiens

RCL1

10171

Affinity Capture-MS

Homo sapiens

KLC3

Two-hybrid

Homo sapiens

HOXA9

Affinity Capture-MS

Homo sapiens

TNNI2

7136

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

IKBKG

8517

Affinity Capture-Western

Homo sapiens

CCDC130

Affinity Capture-MS

Homo sapiens

CUL3

8452

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

KLHL29

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLHL13

90293

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

MAPRE1

22919

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens

AURKB

9212

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens

ZNF777

Affinity Capture-MS

Homo sapiens

TPM1

7168

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HOXC9

Affinity Capture-MS

Homo sapiens

ZBTB3

Affinity Capture-MS

Homo sapiens

SP110

Affinity Capture-MS

Homo sapiens

RPL28

6158

Affinity Capture-MS

Homo sapiens

GLI4

Affinity Capture-MS

Homo sapiens

PLK1

5347

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

COPS5

10987

Affinity Capture-MS

Homo sapiens

HOXD10

Affinity Capture-MS

Homo sapiens

HOXC12

Affinity Capture-MS

Homo sapiens

C9orf72

Affinity Capture-MS

Homo sapiens

PLEKHG3

26030

Affinity Capture-MS

Homo sapiens

IKBKB

3551

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens

NUMB

8650

Affinity Capture-MS

Homo sapiens

CHUK

1147

Affinity Capture-Western

Homo sapiens

ZNF263

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLHL14

57565

Affinity Capture-MS

Homo sapiens

BACH2

60468

Affinity Capture-MS

Homo sapiens

ZBTB44

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which KLHL21 is involved

Pathway	Evidence	Source
Adaptive Immune System	TAS	Reactome
Antigen processing: Ubiquitination & Proteasome degradation	TAS	Reactome
Class I MHC mediated antigen processing & presentation	TAS	Reactome
Immune System	TAS	Reactome
Metabolism of proteins	TAS	Reactome
Neddylation	TAS	Reactome
Post-translational protein modification	TAS	Reactome

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