Gene description for IQSEC1
Gene name IQ motif and Sec7 domain 1
Gene symbol IQSEC1
Other names/aliases ARF-GEP100
ARFGEP100
BRAG2
GEP100
Species Homo sapiens
 Database cross references - IQSEC1
ExoCarta ExoCarta_9922
Vesiclepedia VP_9922
Entrez Gene 9922
HGNC 29112
MIM 610166
UniProt Q6DN90  
 IQSEC1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for IQSEC1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
Biological Process
    actin cytoskeleton organization GO:0030036 IBA
    regulation of ARF protein signal transduction GO:0032012 IEA
    positive regulation of keratinocyte migration GO:0051549 ISS
    dendritic spine development GO:0060996 ISS
    positive regulation of focal adhesion disassembly GO:0120183 ISS
Subcellular Localization
    nucleolus GO:0005730 IDA
    synaptic vesicle GO:0008021 IEA
    postsynaptic density GO:0014069 IEA
    membrane GO:0016020 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified IQSEC1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IQSEC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 DNAJC7 7266
Affinity Capture-MS Homo sapiens
3 FAM196A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MECP2 4204
Affinity Capture-MS Homo sapiens
5 CCDC96  
Affinity Capture-MS Homo sapiens
6 IRAK2  
Affinity Capture-MS Homo sapiens
7 NPAS1  
Affinity Capture-MS Homo sapiens
8 TFDP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MAGEA3  
Two-hybrid Homo sapiens
10 PRMT6  
Affinity Capture-MS Homo sapiens
11 FAM167A  
Affinity Capture-MS Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 RC3H2  
Affinity Capture-MS Homo sapiens
14 WWOX 51741
Affinity Capture-MS Homo sapiens
15 AGO2 27161
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 LRRC25  
Affinity Capture-MS Homo sapiens
17 P4HA2 8974
Affinity Capture-MS Homo sapiens
18 HERC2P9  
Affinity Capture-MS Homo sapiens
19 TRIM66  
Affinity Capture-MS Homo sapiens
20 MFI2 4241
Affinity Capture-MS Homo sapiens
21 HAVCR2  
Affinity Capture-MS Homo sapiens
22 IQSEC2 23096
Affinity Capture-MS Homo sapiens
23 SULT1C4  
Affinity Capture-MS Homo sapiens
24 GNAQ 2776
Affinity Capture-Western Homo sapiens
25 SRPR 6734
Affinity Capture-MS Homo sapiens
26 SKP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 APOE 348
Two-hybrid Homo sapiens
28 IRAK1  
Affinity Capture-MS Homo sapiens
29 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
30 FYCO1 79443
Affinity Capture-MS Homo sapiens
31 ARF6 382
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
32 METTL21B  
Affinity Capture-MS Homo sapiens
33 CACNG7  
Affinity Capture-MS Homo sapiens
34 MAGEA9  
Affinity Capture-MS Homo sapiens
35 RIPK4  
Affinity Capture-MS Homo sapiens
36 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
37 DNAJB6 10049
Affinity Capture-MS Homo sapiens
38 IRF7  
Affinity Capture-MS Homo sapiens
39 C6orf141  
Affinity Capture-MS Homo sapiens
40 CRYBB3  
Affinity Capture-MS Homo sapiens
41 PROZ 8858
Affinity Capture-MS Homo sapiens
42 WDR5 11091
Affinity Capture-MS Homo sapiens
43 BSND  
Affinity Capture-MS Homo sapiens
44 NAAA  
Affinity Capture-MS Homo sapiens
45 BCL2L12 83596
Affinity Capture-MS Homo sapiens
46 APBA2 321
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 D2HGDH 728294
Affinity Capture-MS Homo sapiens
48 BRINP1  
Affinity Capture-MS Homo sapiens
49 FAM83A  
Affinity Capture-MS Homo sapiens
50 B4GALT2  
Affinity Capture-MS Homo sapiens
51 ACTL6B  
Affinity Capture-MS Homo sapiens
52 RNF2  
Affinity Capture-MS Homo sapiens
53 RHOB 388
Proximity Label-MS Homo sapiens
54 FBLN5 10516
Affinity Capture-MS Homo sapiens
55 PELI3  
Affinity Capture-MS Homo sapiens
56 CTNNA1 1495
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
57 DACT2  
Affinity Capture-MS Homo sapiens
58 DDX39B 7919
Affinity Capture-MS Homo sapiens
59 ANKRD28 23243
Affinity Capture-MS Homo sapiens
60 LURAP1  
Affinity Capture-MS Homo sapiens
61 HSPB8 26353
Affinity Capture-MS Homo sapiens
62 GALNT9  
Affinity Capture-MS Homo sapiens
63 ASB4  
Affinity Capture-MS Homo sapiens
64 MLLT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 STK32C  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which IQSEC1 is involved
No pathways found





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