Gene description for ARL2
Gene name ADP-ribosylation factor-like 2
Gene symbol ARL2
Other names/aliases ARFL2
Species Homo sapiens
 Database cross references - ARL2
ExoCarta ExoCarta_402
Vesiclepedia VP_402
Entrez Gene 402
HGNC 693
MIM 601175
UniProt P36404  
 ARL2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for ARL2
Molecular Function
    GTPase activity GO:0003924 IMP
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IMP
    GDP binding GO:0019003 IEA
Biological Process
    regulation of glycolytic process GO:0006110 IMP
    protein folding GO:0006457 IBA
    centrosome cycle GO:0007098 IMP
    positive regulation of cell-substrate adhesion GO:0010811 ISS
    regulation of microtubule polymerization GO:0031113 IMP
    positive regulation of microtubule polymerization GO:0031116 IDA
    negative regulation of GTPase activity GO:0034260 IDA
    maintenance of protein location in nucleus GO:0051457 IDA
    bicellular tight junction assembly GO:0070830 ISS
    regulation of aerobic respiration GO:1903715 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial intermembrane space GO:0005758 IDA
    mitochondrial matrix GO:0005759 TAS
    Golgi apparatus GO:0005794 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IDA
    cilium GO:0005929 IDA
    microtubule cytoskeleton GO:0015630 IBA
    lateral plasma membrane GO:0016328 ISS
 Experiment description of studies that identified ARL2 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ARL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
3 TUBB2A 7280
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GYPA  
Affinity Capture-MS Homo sapiens
5 NT5E 4907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FN1 2335
Affinity Capture-MS Homo sapiens
7 WDR17  
Affinity Capture-MS Homo sapiens
8 GINS2  
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 ARL2BP  
Reconstituted Complex Bos taurus
11 TUBB 203068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
12 Tubb5 22154
Affinity Capture-MS Mus musculus
13 PES1 23481
Co-fractionation Homo sapiens
14 YWHAB 7529
Affinity Capture-MS Homo sapiens
15 RIT1 6016
Negative Genetic Homo sapiens
16 TBCE 6905
Co-fractionation Homo sapiens
17 RPA2 6118
Proximity Label-MS Homo sapiens
18 PTPRA 5786
Affinity Capture-MS Homo sapiens
19 TUBB8 347688
Affinity Capture-MS Homo sapiens
20 LHX4  
Two-hybrid Homo sapiens
21 CFAP36 112942
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 YWHAZ 7534
Affinity Capture-MS Homo sapiens
24 TUBB4B 10383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ARL2BP 23568
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
26 FAHD1 81889
Affinity Capture-MS Homo sapiens
27 COX8A  
Proximity Label-MS Homo sapiens
28 CLPP 8192
Proximity Label-MS Homo sapiens
29 PPP2R2C 5522
Co-purification Homo sapiens
30 PDHA1 5160
Proximity Label-MS Homo sapiens
31 TUBB4A 10382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TBCD 6904
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
33 PDE6D 5147
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 Tubb4b 227613
Affinity Capture-MS Mus musculus
35 ALPI  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NUTM2F  
Affinity Capture-MS Homo sapiens
37 HSCB 150274
Proximity Label-MS Homo sapiens
38 TUBB2B 347733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ARL3 403
Affinity Capture-MS Homo sapiens
40 MOV10 4343
Affinity Capture-RNA Homo sapiens
41 ACOX1 51
Affinity Capture-MS Homo sapiens
42 ESRRA  
Co-fractionation Homo sapiens
43 PPP2R5D 5528
Co-purification Homo sapiens
44 TBL1XR1 79718
Two-hybrid Homo sapiens
45 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PTPDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PPP2CA 5515
Co-purification Homo sapiens
48 UNC119 9094
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
49 TBC1D23 55773
Affinity Capture-MS Homo sapiens
50 KRAS 3845
Negative Genetic Homo sapiens
51 CYCS 54205
Co-fractionation Homo sapiens
52 PTPRU  
Affinity Capture-MS Homo sapiens
53 ARL2 402
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CS 1431
Proximity Label-MS Homo sapiens
55 PPP2CB 5516
Co-purification Homo sapiens
56 L3MBTL4  
Affinity Capture-MS Homo sapiens
57 UNC119B 84747
Affinity Capture-MS Homo sapiens
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