ExoCarta: Gene summary

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Gene description for MKL1
Gene name	megakaryoblastic leukemia (translocation) 1
Gene symbol	MKL1
Other names/aliases	BSAC MAL MRTF-A
Species	Homo sapiens
Database cross references - MKL1
ExoCarta	ExoCarta_57591
Vesiclepedia	VP_57591
Entrez Gene	57591
HGNC	14334
MIM	606078
UniProt	Q969V6
MKL1 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells	33991177
Gastric cancer cells	33991177
Gastric cancer cells	33991177
Hepatocytes	26054723

Gene ontology annotations for MKL1

Molecular Function
	transcription coactivator activity	GO:0003713	IBA
	transcription coactivator activity	GO:0003713	IGI
	transcription coactivator activity	GO:0003713	ISS
	actin binding	GO:0003779	IDA
	actin monomer binding	GO:0003785	ISS
	protein binding	GO:0005515	IPI
	leucine zipper domain binding	GO:0043522	IPI
Biological Process
	actin cytoskeleton organization	GO:0030036	ISS
	wound healing, spreading of cells	GO:0044319	IDA
	positive regulation of transcription by RNA polymerase II	GO:0045944	IBA
	positive regulation of transcription by RNA polymerase II	GO:0045944	IDA
	smooth muscle cell differentiation	GO:0051145	IBA
	smooth muscle cell differentiation	GO:0051145	IMP
	positive regulation of miRNA transcription	GO:1902895	IGI
Subcellular Localization
	nucleus	GO:0005634	IBA
	nucleus	GO:0005634	IDA
	nucleoplasm	GO:0005654	IDA
	nucleoplasm	GO:0005654	TAS
	cytoplasm	GO:0005737	IDA
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS

Experiment description of studies that identified MKL1 in sEVs

Experiment ID

363

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

364

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

365

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

237

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	Enriched markers
✔ HSP90B1	Negative markers
✔ qNano	Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocytes

Sample name

MIHA

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RNA Sequencing

Protein-protein interactions for MKL1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

MVP

9961

Two-hybrid

Homo sapiens

SNX27

81609

Affinity Capture-MS

Homo sapiens

SSRP1

6749

Affinity Capture-Western

Homo sapiens

MYOCD

Reconstituted Complex

Homo sapiens

GSK3B

2932

Affinity Capture-Western

Homo sapiens

XPO1

7514

Affinity Capture-MS

Homo sapiens

BAG2

9532

Affinity Capture-MS

Homo sapiens

NCOA3

Reconstituted Complex

Homo sapiens

MKL2

Affinity Capture-Western

Homo sapiens

ACTC1

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

S100A2

6273

Affinity Capture-MS

Homo sapiens

FHL2

2274

Affinity Capture-MS

Homo sapiens

TRIM54

Two-hybrid

Homo sapiens

NXF1

10482

Affinity Capture-RNA

Homo sapiens

APEX1

328

Affinity Capture-RNA

Homo sapiens

PFN1

5216

Proximity Label-MS

Homo sapiens

SUPT16H

11198

Affinity Capture-Western

Homo sapiens

SMARCA4

6597

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens

HSPA2

3306

Affinity Capture-MS

Homo sapiens

SMAD3

4088

Affinity Capture-Western

Homo sapiens

HSPA1A

3303

Affinity Capture-MS

Homo sapiens

PEX14

5195

Proximity Label-MS

Homo sapiens

SRF

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

ACTB

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

KDM3A

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens

DNMT1

1786

Affinity Capture-Western

Homo sapiens

BCL7A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ACTG1

Affinity Capture-MS

Homo sapiens

EP300

2033

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

View the network

Pathways in which MKL1 is involved

Pathway	Evidence	Source
Metabolism of proteins	TAS	Reactome
Post-translational protein modification	TAS	Reactome
RHO GTPase Effectors	TAS	Reactome
RHO GTPases Activate Formins	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome
SUMO E3 ligases SUMOylate target proteins	TAS	Reactome
SUMOylation	TAS	Reactome
SUMOylation of transcription cofactors	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here