Gene description for ACTG1
Gene name actin gamma 1
Gene symbol ACTG1
Other names/aliases ACT
ACTG
BRWS2
DFNA20
DFNA26
HEL-176
Species Homo sapiens
 Database cross references - ACTG1
ExoCarta ExoCarta_71
Vesiclepedia VP_71
Entrez Gene 71
HGNC 144
MIM 102560
UniProt P63261  
 ACTG1 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 16302729    
Breast cancer cells 19415654    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 22740476    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Keratinocytes 19530224    
Keratinocytes 19530224    
Melanoma cells 15478216    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
Urine 15326289    
Urine 21557262    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for ACTG1
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IC
    protein binding GO:0005515 IPI
    profilin binding GO:0005522 IDA
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
    protein kinase binding GO:0019901 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    identical protein binding GO:0042802 IPI
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IBA
Biological Process
    angiogenesis GO:0001525 IMP
    morphogenesis of a polarized epithelium GO:0001738 IMP
    axonogenesis GO:0007409 IBA
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of cell migration GO:0030335 IMP
    maintenance of blood-brain barrier GO:0035633 NAS
    sarcomere organization GO:0045214 IEA
    cell motility GO:0048870 IBA
    regulation of stress fiber assembly GO:0051492 IMP
    regulation of focal adhesion assembly GO:0051893 IMP
    platelet aggregation GO:0070527 HMP
    cellular response to type II interferon GO:0071346 IEA
    positive regulation of wound healing GO:0090303 IMP
    postsynaptic actin cytoskeleton organization GO:0098974 IEA
    tight junction assembly GO:0120192 IMP
    regulation of transepithelial transport GO:0150111 IMP
    regulation of synaptic vesicle endocytosis GO:1900242 IEA
    protein localization to bicellular tight junction GO:1902396 IMP
Subcellular Localization
    extracellular space GO:0005615 HDA
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 ISS
    cytoskeleton GO:0005856 TAS
    actin filament GO:0005884 IBA
    actin filament GO:0005884 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    focal adhesion GO:0005925 ISS
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    myofibril GO:0030016 IEA
    axon GO:0030424 IBA
    filamentous actin GO:0031941 IEA
    NuA4 histone acetyltransferase complex GO:0035267 IBA
    apical junction complex GO:0043296 IDA
    calyx of Held GO:0044305 IEA
    synapse GO:0045202 IBA
    phagocytic vesicle GO:0045335 IEA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
    blood microparticle GO:0072562 HDA
    dense body GO:0097433 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    basal body patch GO:0120220 IEA
 Experiment description of studies that identified ACTG1 in sEVs
1
Experiment ID 19
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16302729    
Organism Homo sapiens
Experiment description "Purification, characterization and biological significance of tumor-derived exosomes."
Authors "Koga K, Matsumoto K, Akiyoshi T, Kubo M, Yamanaka N, Tasaki A, Nakashima H, Nakamura M, Kuroki S, Tanaka M, Katano M"
Journal name ACR
Publication year 2005
Sample Breast cancer cells
Sample name BT-474
MDA-MB-231
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Filtration
Immunobeads(HER2)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
2
Experiment ID 65
MISEV standards
✘ Biophysical techniques
✔
HSP60|HSP27|GAPDH|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors "Staubach S, Razawi H, Hanisch FG."
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
3
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
8
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 201
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22740476    
Organism Homo sapiens
Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors "Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ."
Journal name Electrophoresis
Publication year 2012
Sample Colorectal cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
14
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
15
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
16
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
17
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 12
MISEV standards
✔
EM
Biophysical techniques
✔
MHCI
Enriched markers
✔
CANX|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15478216    
Organism Homo sapiens
Experiment description Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry.
Authors "Mears R, Craven RA, Hanrahan S, Totty N, Upton C, Young SL, Patel P, Selby PJ, Banks RE"
Journal name PROTEOMICS
Publication year 2004
Sample Melanoma cells
Sample name MeWo
SK-MEL-28
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [4700 Proteomics Analyzer]
Western blotting
20
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
21
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 136
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
25
Experiment ID 137
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB6|CD10
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
26
Experiment ID 138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
27
Experiment ID 139
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
28
Experiment ID 140
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
29
Experiment ID 141
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
30
Experiment ID 142
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
31
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
32
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
33
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
34
Experiment ID 146
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name RWPE - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
35
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
36
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
37
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
39
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
41
Experiment ID 113
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
42
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
43
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
44
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACTG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INSIG1  
Affinity Capture-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 RPS6KA5 9252
Two-hybrid Homo sapiens
4 MNS1 55329
Affinity Capture-MS Homo sapiens
5 DLG5 9231
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 SCIN 85477
Affinity Capture-MS Homo sapiens
8 GATAD2B 57459
Affinity Capture-MS Homo sapiens
9 PPP1CB 5500
Affinity Capture-MS Homo sapiens
10 SPRTN  
Affinity Capture-MS Homo sapiens
11 UBL4A 8266
Affinity Capture-MS Homo sapiens
12 Tpm1 22003
Affinity Capture-MS Mus musculus
13 SHC1 6464
Affinity Capture-MS Homo sapiens
14 WIPF2 147179
Affinity Capture-MS Homo sapiens
15 ARPC5L 81873
Affinity Capture-MS Homo sapiens
16 BCL7C  
Affinity Capture-MS Homo sapiens
17 PTMA 5757
Cross-Linking-MS (XL-MS) Homo sapiens
18 MKL1 57591
Affinity Capture-MS Homo sapiens
19 RIN3  
Affinity Capture-MS Homo sapiens
20 WASF2 10163
Affinity Capture-MS Homo sapiens
21 LIG4 3981
Two-hybrid Homo sapiens
22 ARPC5 10092
Affinity Capture-MS Homo sapiens
23 GPHN 10243
Affinity Capture-MS Homo sapiens
24 SP1  
Affinity Capture-MS Homo sapiens
25 Pparg  
Affinity Capture-MS Mus musculus
26 CAP1 10487
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
27 IKZF1  
Affinity Capture-MS Homo sapiens
28 ACTC1 70
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
29 DBN1 1627
Affinity Capture-MS Homo sapiens
30 MYOC 4653
Two-hybrid Homo sapiens
31 LIMA1 51474
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
32 ACTR1B 10120
Affinity Capture-MS Homo sapiens
33 HRAS 3265
Reconstituted Complex Homo sapiens
34 APOE 348
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
35 ADD3 120
Affinity Capture-MS Homo sapiens
36 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
37 ARCN1 372
Co-fractionation Homo sapiens
38 FMN2  
Affinity Capture-MS Homo sapiens
39 MYO5C 55930
Affinity Capture-MS Homo sapiens
40 TMOD1  
Affinity Capture-MS Homo sapiens
41 FHOD1 29109
Affinity Capture-Western Homo sapiens
42 Actb 11461
Affinity Capture-MS Mus musculus
43 SMARCD2 6603
Affinity Capture-MS Homo sapiens
44 RPS6KB2  
Affinity Capture-MS Homo sapiens
45 VASP 7408
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
46 PSEN2 5664
Two-hybrid Homo sapiens
47 EEF1A2 1917
Co-fractionation Homo sapiens
48 DCTN3 11258
Affinity Capture-MS Homo sapiens
49 POTEF 728378
Cross-Linking-MS (XL-MS) Homo sapiens
50 CCDC53 51019
Affinity Capture-MS Homo sapiens
51 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 HSPA4 3308
Co-fractionation Homo sapiens
53 POTEE 445582
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
54 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 RUVBL1 8607
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
56 WDR76  
Affinity Capture-MS Homo sapiens
57 KIAA1522 57648
Affinity Capture-MS Homo sapiens
58 CORO1B 57175
Co-fractionation Homo sapiens
59 B3GNT2 10678
Affinity Capture-MS Homo sapiens
60 BCL7B  
Affinity Capture-MS Homo sapiens
61 ARHGAP32  
Affinity Capture-MS Homo sapiens
62 TGFB1 7040
Affinity Capture-MS Homo sapiens
63 NDRG1 10397
Affinity Capture-MS Homo sapiens
64 DPF2  
Affinity Capture-MS Homo sapiens
65 DCTN4 51164
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ACTR3 10096
Cross-Linking-MS (XL-MS) Homo sapiens
67 CCT6A 908
Affinity Capture-MS Homo sapiens
68 ACTG2 72
Cross-Linking-MS (XL-MS) Homo sapiens
69 STOM 2040
Affinity Capture-MS Homo sapiens
70 IQGAP1 8826
Affinity Capture-MS Homo sapiens
71 LGR4 55366
Affinity Capture-MS Homo sapiens
72 ACTR1A 10121
Affinity Capture-MS Homo sapiens
73 POU5F1  
Affinity Capture-RNA Homo sapiens
74 TNIK 23043
Two-hybrid Homo sapiens
75 ABI1 10006
Affinity Capture-MS Homo sapiens
76 SMC2 10592
Affinity Capture-MS Homo sapiens
77 WASH1 100287171
Affinity Capture-MS Homo sapiens
78 Flot2 14252
Affinity Capture-MS Mus musculus
79 POTEI 653269
Cross-Linking-MS (XL-MS) Homo sapiens
80 ARID1A 8289
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
81 MAGEA3  
Affinity Capture-MS Homo sapiens
82 NPM1 4869
Proximity Label-MS Homo sapiens
83 PEPD 5184
Co-fractionation Homo sapiens
84 ITGA4 3676
Affinity Capture-MS Homo sapiens
85 EED  
Affinity Capture-MS Homo sapiens
86 UCHL5 51377
Affinity Capture-MS Homo sapiens
87 WASL 8976
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
88 CAPZA1 829
Affinity Capture-MS Homo sapiens
89 SFN 2810
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
90 MTFR2  
Affinity Capture-MS Homo sapiens
91 PFN2 5217
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
92 CTPS1 1503
Co-fractionation Homo sapiens
93 BRK1 55845
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
94 GMFB 2764
Affinity Capture-MS Homo sapiens
95 FAF2 23197
Affinity Capture-MS Homo sapiens
96 FGFR1 2260
Affinity Capture-MS Homo sapiens
97 WASF3 10810
Affinity Capture-MS Homo sapiens
98 CCT5 22948
Affinity Capture-MS Homo sapiens
99 SRCAP  
Affinity Capture-MS Homo sapiens
100 EEF1A1 1915
Co-fractionation Homo sapiens
101 Lima1  
Affinity Capture-MS Mus musculus
102 GIT2 9815
Two-hybrid Homo sapiens
103 Tmed10 68581
Affinity Capture-MS Mus musculus
104 HSPA8 3312
Co-fractionation Homo sapiens
105 WDR1 9948
Affinity Capture-MS Homo sapiens
106 YWHAB 7529
Co-fractionation Homo sapiens
107 INSIG2  
Affinity Capture-MS Homo sapiens
108 RBMX 27316
Co-fractionation Homo sapiens
109 COBLL1 22837
Affinity Capture-MS Homo sapiens
110 PFN1 5216
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
111 ALAS1  
Affinity Capture-MS Homo sapiens
112 ACTA2 59
Cross-Linking-MS (XL-MS) Homo sapiens
113 TOLLIP 54472
Proximity Label-MS Homo sapiens
114 FMNL2 114793
Affinity Capture-MS Homo sapiens
115 MYO18A 399687
Affinity Capture-MS Homo sapiens
116 TCP1 6950
Affinity Capture-MS Homo sapiens
117 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
118 RBX1 9978
Affinity Capture-MS Homo sapiens
119 WASF1 8936
Affinity Capture-MS Homo sapiens
120 MEPCE 56257
Affinity Capture-MS Homo sapiens
121 DCTN5 84516
Affinity Capture-MS Homo sapiens
122 ERBB3 2065
Affinity Capture-MS Homo sapiens
123 CTBP2 1488
Two-hybrid Homo sapiens
124 EVL 51466
Affinity Capture-MS Homo sapiens
125 ARHGAP27  
Affinity Capture-MS Homo sapiens
126 PLD1 5337
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
127 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
128 PSMD14 10213
Affinity Capture-MS Homo sapiens
129 BAIAP2 10458
Affinity Capture-MS Homo sapiens
130 HIST1H3E 8353
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
131 EPS8L2 64787
Affinity Capture-Western Homo sapiens
132 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
133 SH3GL2  
Two-hybrid Homo sapiens
134 UBASH3B 84959
Affinity Capture-MS Homo sapiens
135 MAPT  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
136 SMARCA4 6597
Affinity Capture-MS Homo sapiens
137 VCL 7414
Affinity Capture-MS Homo sapiens
138 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
139 MYH9 4627
Affinity Capture-MS Homo sapiens
140 WDYHV1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
141 TRIM21 6737
Affinity Capture-MS Homo sapiens
142 SLX4  
Affinity Capture-MS Homo sapiens
143 TWF1 5756
Affinity Capture-MS Homo sapiens
144 ACTL6A 86
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
145 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
146 FANCD2  
Affinity Capture-MS Homo sapiens
147 CSRP2BP  
Affinity Capture-MS Homo sapiens
148 FLNB 2317
Co-fractionation Homo sapiens
149 YWHAQ 10971
Affinity Capture-MS Homo sapiens
150 FBXO25  
Affinity Capture-MS Homo sapiens
151 CCDC101  
Affinity Capture-MS Homo sapiens
152 CCDC22 28952
Two-hybrid Homo sapiens
153 GZMK  
Biochemical Activity Homo sapiens
154 Coro1c 23790
Affinity Capture-MS Mus musculus
155 SREBF2 6721
Positive Genetic Homo sapiens
156 ACTR10 55860
Affinity Capture-MS Homo sapiens
157 ENAH 55740
Affinity Capture-MS Homo sapiens
158 ACTG1 71
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
159 MCM2 4171
Affinity Capture-MS Homo sapiens
160 ATP5B 506
Co-fractionation Homo sapiens
161 ST3GAL3  
Two-hybrid Homo sapiens
162 BRCA1 672
Two-hybrid Homo sapiens
163 RAN 5901
Co-fractionation Homo sapiens
164 USP5 8078
Co-fractionation Homo sapiens
165 FBXO30 84085
Affinity Capture-MS Homo sapiens
166 BAIAP2L1 55971
Affinity Capture-MS Homo sapiens
167 PACSIN2 11252
Affinity Capture-MS Homo sapiens
168 ANLN 54443
Affinity Capture-MS Homo sapiens
169 PAICS 10606
Co-fractionation Homo sapiens
170 DISC1 27185
Two-hybrid Homo sapiens
171 GAN 8139
Affinity Capture-MS Homo sapiens
172 HSPA5 3309
Affinity Capture-MS Homo sapiens
173 MTFR1  
Affinity Capture-MS Homo sapiens
174 PSMA3 5684
Affinity Capture-MS Homo sapiens
175 SPATS2L 26010
Affinity Capture-MS Homo sapiens
176 Myh9 17886
Affinity Capture-MS Mus musculus
177 CYFIP2 26999
Affinity Capture-MS Homo sapiens
178 ARPC1B 10095
Affinity Capture-MS Homo sapiens
179 EIF6 3692
Two-hybrid Homo sapiens
180 RC3H2  
Affinity Capture-MS Homo sapiens
181 E2F8  
Co-fractionation Homo sapiens
182 ARPC3 10094
Affinity Capture-MS Homo sapiens
183 IKZF3  
Affinity Capture-MS Homo sapiens
184 NCKAP1 10787
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
185 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
186 CTNNB1 1499
Affinity Capture-MS Homo sapiens
187 CKAP2  
Affinity Capture-MS Homo sapiens
188 ABI2 10152
Affinity Capture-MS Homo sapiens
189 BCAP31 10134
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
190 FAM21A 387680
Affinity Capture-MS Homo sapiens
191 ACTBL2 345651
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
192 NR3C1 2908
Affinity Capture-MS Homo sapiens
193 BTF3 689
Affinity Capture-MS Homo sapiens
194 CCT4 10575
Affinity Capture-MS Homo sapiens
195 CCT3 7203
Affinity Capture-MS Homo sapiens
196 MYO19  
Affinity Capture-MS Homo sapiens
197 HOOK3 84376
Proximity Label-MS Homo sapiens
198 MRGBP  
Affinity Capture-MS Homo sapiens
199 FBXO6 26270
Affinity Capture-MS Homo sapiens
200 EP400  
Affinity Capture-MS Homo sapiens
201 TMEM11 8834
Affinity Capture-MS Homo sapiens
202 MORF4L1  
Affinity Capture-MS Homo sapiens
203 GZMA 3001
Biochemical Activity Homo sapiens
204 CORO1C 23603
Co-fractionation Homo sapiens
205 SUZ12  
Affinity Capture-MS Homo sapiens
206 LARS 51520
Affinity Capture-MS Homo sapiens
207 MYO1C 4641
Affinity Capture-MS Homo sapiens
208 CYFIP1 23191
Affinity Capture-MS Homo sapiens
209 G6PD 2539
Co-fractionation Homo sapiens
210 ARID1B 57492
Affinity Capture-MS Homo sapiens
211 CDK2 1017
Affinity Capture-MS Homo sapiens
212 MAPK6  
Two-hybrid Homo sapiens
213 SMARCD1 6602
Affinity Capture-MS Homo sapiens
214 ADD1 118
Affinity Capture-MS Homo sapiens
215 Flnb 286940
Affinity Capture-MS Mus musculus
216 EPS8L1 54869
Affinity Capture-Western Homo sapiens
217 SERBP1 26135
Affinity Capture-MS Homo sapiens
218 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
219 SPOP  
Affinity Capture-MS Homo sapiens
220 FDPS 2224
Co-fractionation Homo sapiens
221 TMOD2 29767
Affinity Capture-MS Homo sapiens
222 YARS 8565
Co-fractionation Homo sapiens
223 CCT8 10694
Affinity Capture-MS Homo sapiens
224 MRFAP1  
Affinity Capture-MS Homo sapiens
225 CFL1 1072
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
226 MYO9B 4650
Affinity Capture-MS Homo sapiens
227 KAT5  
Affinity Capture-MS Homo sapiens
228 CFL2 1073
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
229 TRAF2 7186
Co-localization Homo sapiens
230 DSTN 11034
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
231 ACTB 60
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
232 FLII 2314
Affinity Capture-MS Homo sapiens
233 DDRGK1 65992
Affinity Capture-MS Homo sapiens
234 RNF2  
Affinity Capture-MS Homo sapiens
235 BMI1  
Affinity Capture-MS Homo sapiens
236 WDR44 54521
Affinity Capture-MS Homo sapiens
237 KRAS 3845
Affinity Capture-MS Homo sapiens
238 DCTN1 1639
Affinity Capture-MS Homo sapiens
239 RNF208  
Affinity Capture-MS Homo sapiens
240 PXK 54899
Affinity Capture-MS Homo sapiens
241 C9orf72  
Affinity Capture-MS Homo sapiens
242 TSGA10  
Affinity Capture-MS Homo sapiens
243 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
244 CCDC8  
Affinity Capture-MS Homo sapiens
245 WHAMM  
Affinity Capture-MS Homo sapiens
246 SPTBN1 6711
Affinity Capture-MS Homo sapiens
247 SPIRE2  
Affinity Capture-MS Homo sapiens
248 Calml3  
Affinity Capture-MS Mus musculus
249 GSN 2934
Affinity Capture-MS Homo sapiens
250 DUSP19  
Affinity Capture-MS Homo sapiens
251 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
252 SMARCC2 6601
Affinity Capture-MS Homo sapiens
253 CPNE3 8895
Co-fractionation Homo sapiens
254 FEZ1  
Affinity Capture-Western Homo sapiens
255 PDLIM7 9260
Affinity Capture-MS Homo sapiens
256 EIF3F 8665
Affinity Capture-MS Homo sapiens
257 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
258 FASN 2194
Positive Genetic Homo sapiens
259 SPTAN1 6709
Affinity Capture-MS Homo sapiens
260 Tmod3 50875
Affinity Capture-MS Mus musculus
261 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
262 HSPB2  
Two-hybrid Homo sapiens
263 PLEC 5339
Cross-Linking-MS (XL-MS) Homo sapiens
264 PDCL3 79031
Affinity Capture-MS Homo sapiens
265 WIPF3  
Affinity Capture-MS Homo sapiens
266 ILF3 3609
Affinity Capture-RNA Homo sapiens
267 TOPBP1 11073
Affinity Capture-MS Homo sapiens
268 TP53 7157
Affinity Capture-MS Homo sapiens
269 POTEJ 653781
Cross-Linking-MS (XL-MS) Homo sapiens
270 MDK 4192
Affinity Capture-MS Homo sapiens
271 RUVBL2 10856
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
272 TPR 7175
Co-fractionation Homo sapiens
273 SMARCE1 6605
Affinity Capture-MS Homo sapiens
274 SMARCC1 6599
Affinity Capture-MS Homo sapiens
275 EHHADH 1962
Two-hybrid Homo sapiens
276 CCT2 10576
Affinity Capture-MS Homo sapiens
277 Myo1c 17913
Affinity Capture-MS Mus musculus
278 CDC37 11140
Two-hybrid Homo sapiens
279 ARPC2 10109
Affinity Capture-MS Homo sapiens
280 SS18  
Affinity Capture-MS Homo sapiens
281 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
282 PHACTR2  
Affinity Capture-MS Homo sapiens
283 C17orf89  
Affinity Capture-MS Homo sapiens
284 MORF4L2  
Affinity Capture-MS Homo sapiens
285 DCTN2 10540
Affinity Capture-MS Homo sapiens
286 CAP2 10486
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
287 MLH1 4292
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
288 DMAP1 55929
Affinity Capture-MS Homo sapiens
289 PRR11  
Affinity Capture-MS Homo sapiens
290 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
291 COBL  
Affinity Capture-MS Homo sapiens
292 DDX58 23586
Affinity Capture-RNA Homo sapiens
293 MKL2  
Affinity Capture-MS Homo sapiens
294 CCT7 10574
Affinity Capture-MS Homo sapiens
295 Myh10 77579
Affinity Capture-MS Mus musculus
296 TLR9  
Affinity Capture-MS Homo sapiens
297 UFL1 23376
Affinity Capture-MS Homo sapiens
298 ADRB2  
Affinity Capture-MS Homo sapiens
299 ACTA1 58
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
300 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
301 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
302 NCL 4691
Co-fractionation Homo sapiens
303 EZH2  
Affinity Capture-MS Homo sapiens
304 PHACTR4 65979
Affinity Capture-MS Homo sapiens
305 INF2 64423
Affinity Capture-MS Homo sapiens
306 LRRK2 120892
Affinity Capture-MS Homo sapiens
307 ERRFI1 54206
Affinity Capture-MS Homo sapiens
308 CAD 790
Co-fractionation Homo sapiens
309 PYGB 5834
Co-fractionation Homo sapiens
310 FLNA 2316
Affinity Capture-MS Homo sapiens
311 SMARCB1 6598
Affinity Capture-MS Homo sapiens
312 ACO2 50
Affinity Capture-MS Homo sapiens
313 Flot1 14251
Affinity Capture-MS Mus musculus
314 LDLR 3949
Positive Genetic Homo sapiens
315 TMSB4X 7114
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
316 LRRFIP1 9208
Affinity Capture-MS Homo sapiens
317 UBE2Q2 92912
Affinity Capture-MS Homo sapiens
318 MAP3K4 4216
Affinity Capture-MS Homo sapiens
319 MYC  
Affinity Capture-MS Homo sapiens
320 TWF2 11344
Affinity Capture-MS Homo sapiens
321 SPIRE1 56907
Affinity Capture-MS Homo sapiens
322 PRSS23 11098
Two-hybrid Homo sapiens
323 BCL7A  
Affinity Capture-MS Homo sapiens
324 ARHGAP12  
Affinity Capture-MS Homo sapiens
325 CDKN2A 1029
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
326 CTNND1 1500
Affinity Capture-Western Homo sapiens
327 COTL1 23406
Reconstituted Complex Homo sapiens
328 CEBPA  
Protein-peptide Homo sapiens
View the network image/svg+xml
 Pathways in which ACTG1 is involved
PathwayEvidenceSource
Adherens junctions interactions TAS Reactome
Adherens junctions interactions IEA Reactome
Axon guidance TAS Reactome
Axon guidance IEA Reactome
Cell junction organization TAS Reactome
Cell junction organization IEA Reactome
Cell-Cell communication TAS Reactome
Cell-Cell communication IEA Reactome
Cell-cell junction organization TAS Reactome
Cell-cell junction organization IEA Reactome
Cell-extracellular matrix interactions TAS Reactome
Clathrin-mediated endocytosis TAS Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Disease IEA Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
EPHB-mediated forward signaling TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis IEA Reactome
Formation of annular gap junctions TAS Reactome
Gap junction degradation TAS Reactome
Gap junction trafficking TAS Reactome
Gap junction trafficking and regulation TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Interaction between L1 and Ankyrins TAS Reactome
Interaction between L1 and Ankyrins IEA Reactome
L1CAM interactions TAS Reactome
L1CAM interactions IEA Reactome
Leishmania infection TAS Reactome
Leishmania infection IEA Reactome
Leishmania phagocytosis TAS Reactome
Leishmania phagocytosis IEA Reactome
MAP2K and MAPK activation TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Membrane Trafficking TAS Reactome
Membrane Trafficking IEA Reactome
Nervous system development TAS Reactome
Nervous system development IEA Reactome
Oncogenic MAPK signaling TAS Reactome
Paradoxical activation of RAF signaling by kinase inactive BRAF TAS Reactome
Parasite infection TAS Reactome
Parasite infection IEA Reactome
Parasitic Infection Pathways TAS Reactome
Parasitic Infection Pathways IEA Reactome
RAF/MAP kinase cascade TAS Reactome
Recycling pathway of L1 TAS Reactome
Recycling pathway of L1 IEA Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins TAS Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs TAS Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RHOBTB GTPase Cycle TAS Reactome
RHOBTB2 GTPase cycle TAS Reactome
Sensory Perception IEA Reactome
Sensory processing of sound IEA Reactome
Sensory processing of sound by inner hair cells of the cochlea IEA Reactome
Sensory processing of sound by outer hair cells of the cochlea IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by BRAF and RAF1 fusions TAS Reactome
Signaling by high-kinase activity BRAF mutants TAS Reactome
Signaling by moderate kinase activity BRAF mutants TAS Reactome
Signaling by RAF1 mutants TAS Reactome
Signaling by RAS mutants TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF TAS Reactome
Signaling downstream of RAS mutants TAS Reactome
Translocation of SLC2A4 (GLUT4) to the plasma membrane IEA Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
Vesicle-mediated transport TAS Reactome
Vesicle-mediated transport IEA Reactome





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