Gene description for FADD
Gene name Fas (TNFRSF6)-associated via death domain
Gene symbol FADD
Other names/aliases GIG3
MORT1
Species Homo sapiens
 Database cross references - FADD
ExoCarta ExoCarta_8772
Vesiclepedia VP_8772
Entrez Gene 8772
HGNC 3573
MIM 602457
UniProt Q13158  
 FADD identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for FADD
Molecular Function
    protease binding GO:0002020 IPI
    death receptor binding GO:0005123 IBA
    tumor necrosis factor receptor binding GO:0005164 IEA
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IDA
    tumor necrosis factor receptor superfamily binding GO:0032813 IPI
    receptor serine/threonine kinase binding GO:0033612 IEA
    signaling adaptor activity GO:0035591 IEA
    death effector domain binding GO:0035877 IPI
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 IEA
    caspase binding GO:0089720 IBA
    caspase binding GO:0089720 IDA
    caspase binding GO:0089720 IPI
Biological Process
    kidney development GO:0001822 IEA
    positive regulation of T cell mediated cytotoxicity GO:0001916 ISS
    positive regulation of adaptive immune response GO:0002821 ISS
    apoptotic process GO:0006915 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IDA
    positive regulation of type II interferon production GO:0032729 ISS
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    T cell differentiation in thymus GO:0033077 ISS
    TRAIL-activated apoptotic signaling pathway GO:0036462 IDA
    positive regulation of activated T cell proliferation GO:0042104 ISS
    T cell homeostasis GO:0043029 ISS
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEP
    innate immune response GO:0045087 IEA
    positive regulation of innate immune response GO:0045089 IBA
    positive regulation of macrophage differentiation GO:0045651 IMP
    positive regulation of proteolysis GO:0045862 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    behavioral response to cocaine GO:0048148 IEA
    lymph node development GO:0048535 ISS
    spleen development GO:0048536 ISS
    thymus development GO:0048538 ISS
    defense response to virus GO:0051607 IMP
    positive regulation of type I interferon-mediated signaling pathway GO:0060340 IMP
    negative regulation of necroptotic process GO:0060546 IEA
    negative regulation of activation-induced cell death of T cells GO:0070236 ISS
    cellular response to mechanical stimulus GO:0071260 IEP
    death-inducing signaling complex assembly GO:0071550 IDA
    motor neuron apoptotic process GO:0097049 IEA
    apoptotic signaling pathway GO:0097190 IDA
    extrinsic apoptotic signaling pathway GO:0097191 IBA
    extrinsic apoptotic signaling pathway GO:0097191 IDA
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IEA
    activation of cysteine-type endopeptidase activity GO:0097202 IDA
    necroptotic signaling pathway GO:0097527 IMP
    positive regulation of execution phase of apoptosis GO:1900119 IEA
    positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation GO:2000454 ISS
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cytoplasmic side of plasma membrane GO:0009898 IC
    death-inducing signaling complex GO:0031264 IDA
    death-inducing signaling complex GO:0031264 TAS
    CD95 death-inducing signaling complex GO:0031265 IBA
    CD95 death-inducing signaling complex GO:0031265 IDA
    cell body GO:0044297 IEA
    ripoptosome GO:0097342 IDA
 Experiment description of studies that identified FADD in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for FADD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
2 ABCA1 19
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 APOA1BP 128240
Co-fractionation Homo sapiens
5 BTG1 694
Two-hybrid Homo sapiens
6 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
7 FADD 8772
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
8 MKRN1 23608
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 NEFH 4744
Affinity Capture-MS Homo sapiens
10 SDCBP 6386
Two-hybrid Homo sapiens
11 TRAF2 7186
Affinity Capture-Western Homo sapiens
12 RALGDS  
Affinity Capture-Western Homo sapiens
13 CFLAR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 IL37  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EHD1 10938
Co-fractionation Homo sapiens
16 TNFSF10 8743
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 DAP3 7818
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
18 ATG5 9474
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
19 FAS 355
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
20 TNF  
Affinity Capture-Western Homo sapiens
21 IRF7  
Affinity Capture-Western Homo sapiens
22 SQSTM1 8878
Affinity Capture-Western Homo sapiens
23 E2F4  
Positive Genetic Homo sapiens
24 MYD88 4615
Two-hybrid Homo sapiens
25 NACA 4666
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 MBD4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
27 PRKCZ 5590
Affinity Capture-Western Homo sapiens
28 ATG12  
Affinity Capture-Western Homo sapiens
29 HIPK3 10114
Affinity Capture-Western Homo sapiens
30 FASLG 356
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-localization Homo sapiens
31 CASP8 841
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 NOL3 8996
Phenotypic Suppression Homo sapiens
33 CUL3 8452
Affinity Capture-Western Homo sapiens
34 PIDD1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 BID  
Co-purification Homo sapiens
Co-localization Homo sapiens
36 TAB1 10454
Two-hybrid Homo sapiens
37 MOB4 25843
Two-hybrid Homo sapiens
38 MAVS 57506
Affinity Capture-Western Homo sapiens
39 BTK 695
Negative Genetic Homo sapiens
40 RIPK1 8737
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
41 PARK7 11315
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
42 ZMYM5 9205
Two-hybrid Homo sapiens
43 TCEA2  
Two-hybrid Homo sapiens
44 ZCCHC12  
Two-hybrid Homo sapiens
45 NLRC4  
Affinity Capture-Western Homo sapiens
46 Naca 17938
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
47 CLIP3  
Affinity Capture-Western Homo sapiens
48 ARHGDIA 396
Co-purification Homo sapiens
49 RIPK3 11035
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 TPPP 11076
Affinity Capture-MS Homo sapiens
51 NUTM2F  
Two-hybrid Homo sapiens
52 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
53 UBE2I 7329
Two-hybrid Homo sapiens
54 DAPK1 1612
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
55 IRF3 3661
Negative Genetic Homo sapiens
56 PRKRIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 LRRK2 120892
Affinity Capture-Western Homo sapiens
58 SPOP  
Two-hybrid Homo sapiens
59 TRIM21 6737
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
60 NTF4  
Affinity Capture-MS Homo sapiens
61 TLR3  
Affinity Capture-Western Homo sapiens
62 SDS  
Affinity Capture-MS Homo sapiens
63 CASP10  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
64 RAP1A 5906
Reconstituted Complex Homo sapiens
65 Fas 14102
Two-hybrid Mus musculus
66 EZR 7430
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
67 TRADD 8717
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 KHDRBS1 10657
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
69 DDIT4L  
Two-hybrid Homo sapiens
70 CASP8AP2  
Affinity Capture-Western Homo sapiens
71 MSN 4478
Co-purification Homo sapiens
72 RHOA 387
Co-purification Homo sapiens
Co-localization Homo sapiens
73 MAPK8 5599
Co-purification Homo sapiens
74 SKP2  
Affinity Capture-Western Homo sapiens
75 PEA15 8682
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
76 DEDD  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
77 ANKRD13A 88455
Affinity Capture-Western Homo sapiens
78 RYBP  
Affinity Capture-Western Homo sapiens
79 DAXX  
Affinity Capture-Western Homo sapiens
80 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
81 FAF1 11124
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
82 IKBKG 8517
Affinity Capture-Western Homo sapiens
83 EDAR  
Affinity Capture-Western Homo sapiens
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