Gene description for TRADD
Gene name TNFRSF1A-associated via death domain
Gene symbol TRADD
Other names/aliases Hs.89862
Species Homo sapiens
 Database cross references - TRADD
ExoCarta ExoCarta_8717
Vesiclepedia VP_8717
Entrez Gene 8717
HGNC 12030
MIM 603500
UniProt Q15628  
 TRADD identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for TRADD
Molecular Function
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IBA
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IPI
    tumor necrosis factor receptor binding GO:0005164 IEA
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IPI
    protein-macromolecule adaptor activity GO:0030674 IDA
    signaling adaptor activity GO:0035591 ISS
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 IEA
    death domain binding GO:0070513 IPI
Biological Process
    apoptotic process GO:0006915 TAS
    signal transduction GO:0007165 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IEA
    canonical NF-kappaB signal transduction GO:0007249 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 TAS
    positive regulation of cell migration GO:0030335 IMP
    tumor necrosis factor-mediated signaling pathway GO:0033209 IDA
    tumor necrosis factor-mediated signaling pathway GO:0033209 IEP
    tumor necrosis factor-mediated signaling pathway GO:0033209 TAS
    TRAIL-activated apoptotic signaling pathway GO:0036462 IDA
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 TAS
    positive regulation of inflammatory response GO:0050729 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    positive regulation of hair follicle development GO:0051798 IEA
    cellular response to tumor necrosis factor GO:0071356 IMP
    extrinsic apoptotic signaling pathway GO:0097191 IBA
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    extrinsic apoptotic signaling pathway GO:0097191 TAS
Subcellular Localization
    tumor necrosis factor receptor superfamily complex GO:0002947 IBA
    tumor necrosis factor receptor superfamily complex GO:0002947 IDA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IDA
    cytoplasmic side of plasma membrane GO:0009898 IC
    death-inducing signaling complex GO:0031264 TAS
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified TRADD in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRADD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 KRT18 3875
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 USP2  
Affinity Capture-Western Homo sapiens
4 CASP8 841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 FADD 8772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 IFIT3 3437
Affinity Capture-Western Homo sapiens
7 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
8 TNFRSF1B  
Affinity Capture-Western Homo sapiens
9 TNFRSF25  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 Tnfrsf1a 21937
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
11 FAS 355
Affinity Capture-Western Homo sapiens
12 TNF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 LMP1 3783750
Affinity Capture-Western
14 S100A6 6277
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 Pparg  
Affinity Capture-MS Mus musculus
17 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
18 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 DAB2IP 153090
Affinity Capture-Western Homo sapiens
20 TRADD 8717
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
21 RNMT 8731
Co-fractionation Homo sapiens
22 PDLIM7 9260
Affinity Capture-Western Homo sapiens
23 BPNT1 10380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 BCL10  
Affinity Capture-Western Homo sapiens
25 RIPK1 8737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 DDX58 23586
Affinity Capture-RNA Homo sapiens
27 OPTN 10133
Affinity Capture-Western Homo sapiens
28 TRIP12 9320
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 TOMM70A 9868
Affinity Capture-Western Homo sapiens
30 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
31 STAT1 6772
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
32 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
33 TNFRSF21 27242
Affinity Capture-Western Homo sapiens
34 NGFR 4804
Affinity Capture-Western Homo sapiens
35 NEDD1 121441
Affinity Capture-MS Homo sapiens
36 RXRA 6256
Co-fractionation Homo sapiens
37 APBB2  
Two-hybrid Homo sapiens
38 ITGB4 3691
Co-fractionation Homo sapiens
39 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
40 CDKN2A 1029
Affinity Capture-Western Homo sapiens
41 HIPK2 28996
Affinity Capture-Western Homo sapiens
42 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
43 ZMYND11 10771
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 CHUK 1147
Co-fractionation Homo sapiens
45 MAP3K7  
Co-fractionation Homo sapiens
46 NOL3 8996
Phenotypic Suppression Homo sapiens
47 CAV1 857
Affinity Capture-Western Homo sapiens
48 EDAR  
Affinity Capture-Western Homo sapiens
49 APP 351
Co-fractionation Homo sapiens
50 LRRK2 120892
Affinity Capture-Western Homo sapiens
51 TRPC4AP  
Affinity Capture-Western Homo sapiens
52 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
53 Nedd1  
Affinity Capture-MS Mus musculus
54 TRAF1 7185
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 HYPM  
Affinity Capture-MS Homo sapiens
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