Gene description for ARHGAP18
Gene name Rho GTPase activating protein 18
Gene symbol ARHGAP18
Other names/aliases MacGAP
SENEX
bA307O14.2
Species Homo sapiens
 Database cross references - ARHGAP18
ExoCarta ExoCarta_93663
Vesiclepedia VP_93663
Entrez Gene 93663
HGNC 21035
MIM 613351
UniProt Q8N392  
 ARHGAP18 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ARHGAP18
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IMP
    GTPase activator activity GO:0005096 TAS
    cadherin binding GO:0045296 HDA
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IMP
    regulation of cell shape GO:0008360 IMP
    regulation of actin filament polymerization GO:0030833 IBA
    regulation of actin filament polymerization GO:0030833 IMP
    regulation of actin cytoskeleton organization GO:0032956 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    regulation of cell motility GO:2000145 IMP
Subcellular Localization
    ruffle GO:0001726 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IMP
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoplasmic microtubule GO:0005881 IDA
    plasma membrane GO:0005886 IDA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified ARHGAP18 in sEVs
1
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP2S1 1175
Affinity Capture-MS Homo sapiens
2 ENPP4 22875
Affinity Capture-MS Homo sapiens
3 WDR6 11180
Affinity Capture-MS Homo sapiens
4 AP2B1 163
Affinity Capture-MS Homo sapiens
5 SPRTN  
Affinity Capture-MS Homo sapiens
6 MED17  
Affinity Capture-MS Homo sapiens
7 FGD2 221472
Affinity Capture-MS Homo sapiens
8 PREX2  
Affinity Capture-MS Homo sapiens
9 GAK 2580
Affinity Capture-MS Homo sapiens
10 CD2AP 23607
Affinity Capture-MS Homo sapiens
11 EPS15 2060
Affinity Capture-MS Homo sapiens
12 LSM4 25804
Affinity Capture-MS Homo sapiens
13 CALCOCO1 57658
Affinity Capture-MS Homo sapiens
14 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
15 SMAP2 64744
Affinity Capture-MS Homo sapiens
16 CLTB 1212
Affinity Capture-MS Homo sapiens
17 AFTPH  
Affinity Capture-MS Homo sapiens
18 DNAJB6 10049
Affinity Capture-MS Homo sapiens
19 EIF4ENIF1 56478
Affinity Capture-MS Homo sapiens
20 HSPA2 3306
Affinity Capture-MS Homo sapiens
21 EDC3 80153
Affinity Capture-MS Homo sapiens
22 HELZ 9931
Affinity Capture-MS Homo sapiens
23 CHMP5 51510
Affinity Capture-MS Homo sapiens
24 ANKRD49  
Affinity Capture-MS Homo sapiens
25 AP1G1 164
Affinity Capture-MS Homo sapiens
26 CPSF7 79869
Affinity Capture-MS Homo sapiens
27 SERPINB1 1992
Co-fractionation Homo sapiens
28 AP1AR 55435
Affinity Capture-MS Homo sapiens
29 DDX6 1656
Affinity Capture-MS Homo sapiens
30 GTSE1 51512
Affinity Capture-MS Homo sapiens
31 ANAPC16  
Affinity Capture-MS Homo sapiens
32 TFRC 7037
Affinity Capture-MS Homo sapiens
33 LSM1 27257
Affinity Capture-MS Homo sapiens
34 DCP1B  
Affinity Capture-MS Homo sapiens
35 HSPA8 3312
Affinity Capture-MS Homo sapiens
36 LSM2 57819
Affinity Capture-MS Homo sapiens
37 EDC4 23644
Affinity Capture-MS Homo sapiens
38 ZNF444  
Affinity Capture-MS Homo sapiens
39 PNRC1 10957
Affinity Capture-MS Homo sapiens
40 STOM 2040
Affinity Capture-MS Homo sapiens
41 NBR1 4077
Affinity Capture-MS Homo sapiens
42 XRN1 54464
Affinity Capture-MS Homo sapiens
43 PSMD14 10213
Affinity Capture-MS Homo sapiens
44 GOLGA2 2801
Affinity Capture-MS Homo sapiens
45 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
46 KDM4B  
Affinity Capture-MS Homo sapiens
47 CDH1 999
Proximity Label-MS Homo sapiens
48 BBS1 582
Affinity Capture-MS Homo sapiens
49 DCP1A 55802
Affinity Capture-MS Homo sapiens
50 AP1B1 162
Affinity Capture-MS Homo sapiens
51 PEX14 5195
Proximity Label-MS Homo sapiens
52 PATL1 219988
Affinity Capture-MS Homo sapiens
53 BMP2K 55589
Affinity Capture-MS Homo sapiens
54 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
55 PICALM 8301
Affinity Capture-MS Homo sapiens
56 RYBP  
Affinity Capture-MS Homo sapiens
57 MEA1  
Affinity Capture-MS Homo sapiens
58 ZBTB2 57621
Affinity Capture-MS Homo sapiens
59 IQSEC1 9922
Affinity Capture-MS Homo sapiens
60 RABGGTA 5875
Affinity Capture-MS Homo sapiens
61 CLINT1 9685
Affinity Capture-MS Homo sapiens
62 BAG2 9532
Affinity Capture-MS Homo sapiens
63 SEC13 6396
Affinity Capture-MS Homo sapiens
64 DOK4 55715
Affinity Capture-MS Homo sapiens
65 AP1M1 8907
Affinity Capture-MS Homo sapiens
66 CD9 928
Cross-Linking-MS (XL-MS) Homo sapiens
67 TNRC6B  
Affinity Capture-MS Homo sapiens
68 KIAA0430  
Affinity Capture-MS Homo sapiens
69 AP2M1 1173
Affinity Capture-MS Homo sapiens
70 IST1 9798
Affinity Capture-MS Homo sapiens
71 KIAA2022  
Cross-Linking-MS (XL-MS) Homo sapiens
72 SEC16A 9919
Affinity Capture-MS Homo sapiens
73 AP2A2 161
Affinity Capture-MS Homo sapiens
74 HIP1 3092
Affinity Capture-MS Homo sapiens
75 C6orf10 10665
Affinity Capture-MS Homo sapiens
76 RHOB 388
Proximity Label-MS Homo sapiens
77 CLTC 1213
Affinity Capture-MS Homo sapiens
78 HSPA9 3313
Affinity Capture-MS Homo sapiens
79 MAP7D2 256714
Affinity Capture-MS Homo sapiens
80 BAG4  
Affinity Capture-MS Homo sapiens
81 SYNRG 11276
Affinity Capture-MS Homo sapiens
82 YTHDC2 64848
Affinity Capture-MS Homo sapiens
83 NUDT21 11051
Affinity Capture-MS Homo sapiens
84 MRPL42  
Affinity Capture-MS Homo sapiens
85 MPP6 51678
Two-hybrid Homo sapiens
86 SCYL2 55681
Affinity Capture-MS Homo sapiens
87 LSM14A 26065
Affinity Capture-MS Homo sapiens
88 SMAD4  
Affinity Capture-MS Homo sapiens
89 ZBTB10 65986
Affinity Capture-MS Homo sapiens
90 PLEKHG4B 153478
Affinity Capture-MS Homo sapiens
91 EPN2 22905
Affinity Capture-MS Homo sapiens
92 REPS1 85021
Affinity Capture-MS Homo sapiens
93 MND1  
Affinity Capture-MS Homo sapiens
94 CLTA 1211
Affinity Capture-MS Homo sapiens
95 LSM6 11157
Affinity Capture-MS Homo sapiens
96 JAK2 3717
Affinity Capture-MS Homo sapiens
97 DCP2  
Affinity Capture-MS Homo sapiens
98 HSPA1B 3304
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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