Gene description for SEC16A
Gene name SEC16 homolog A (S. cerevisiae)
Gene symbol SEC16A
Other names/aliases KIAA0310
SEC16L
p250
Species Homo sapiens
 Database cross references - SEC16A
ExoCarta ExoCarta_9919
Vesiclepedia VP_9919
Entrez Gene 9919
HGNC 29006
MIM 612854
UniProt O15027  
 SEC16A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for SEC16A
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    regulation of COPII vesicle coating GO:0003400 IMP
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    Golgi to plasma membrane transport GO:0006893 IMP
    endoplasmic reticulum organization GO:0007029 IDA
    endoplasmic reticulum organization GO:0007029 IMP
    Golgi organization GO:0007030 IBA
    substantia nigra development GO:0021762 HEP
    protein exit from endoplasmic reticulum GO:0032527 ISS
    response to endoplasmic reticulum stress GO:0034976 IDA
    protein stabilization GO:0050821 IMP
    protein localization to endoplasmic reticulum exit site GO:0070973 IBA
    protein localization to endoplasmic reticulum exit site GO:0070973 IMP
    protein localization to plasma membrane GO:0072659 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum membrane GO:0005789 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ER to Golgi transport vesicle membrane GO:0012507 IBA
    organelle membrane GO:0031090 IDA
    perinuclear region of cytoplasm GO:0048471 ISS
    endoplasmic reticulum exit site GO:0070971 IBA
    endoplasmic reticulum exit site GO:0070971 IDA
 Experiment description of studies that identified SEC16A in sEVs
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
13
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
21
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
22
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 434
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 435
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SEC16A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CHD6 84181
Affinity Capture-MS Homo sapiens
3 ZKSCAN1  
Affinity Capture-MS Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 FOXC1  
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 AP2B1 163
Proximity Label-MS Homo sapiens
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
9 KLK15  
Affinity Capture-MS Homo sapiens
10 ETV6  
Proximity Label-MS Homo sapiens
11 BAG3 9531
Affinity Capture-MS Homo sapiens
12 SHC1 6464
Affinity Capture-MS Homo sapiens
13 AIPL1  
Affinity Capture-MS Homo sapiens
14 METTL7A 25840
Proximity Label-MS Homo sapiens
15 LAMP3  
Proximity Label-MS Homo sapiens
16 SOX4  
Proximity Label-MS Homo sapiens
17 KLF9 687
Proximity Label-MS Homo sapiens
18 GJA1 2697
Proximity Label-MS Homo sapiens
19 PKP2 5318
Affinity Capture-MS Homo sapiens
20 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 P4HA2 8974
Affinity Capture-MS Homo sapiens
22 Calml3  
Affinity Capture-MS Mus musculus
23 EBNA-LP  
Affinity Capture-MS
24 DBN1 1627
Affinity Capture-MS Homo sapiens
25 TSG101 7251
Affinity Capture-MS Homo sapiens
26 KIF20A 10112
Affinity Capture-MS Homo sapiens
27 HSD17B11 51170
Proximity Label-MS Homo sapiens
28 EYA2  
Proximity Label-MS Homo sapiens
29 CAND1 55832
Affinity Capture-MS Homo sapiens
30 FIP1L1 81608
Affinity Capture-MS Homo sapiens
31 PTPN1 5770
Proximity Label-MS Homo sapiens
32 Actb 11461
Affinity Capture-MS Mus musculus
33 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 STX4 6810
Proximity Label-MS Homo sapiens
35 ANKRD28 23243
Proximity Label-MS Homo sapiens
36 Nhsl1  
Affinity Capture-MS Mus musculus
37 FAM83D 81610
Affinity Capture-MS Homo sapiens
38 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
39 BAG5 9529
Affinity Capture-MS Homo sapiens
40 EIF4ENIF1 56478
Affinity Capture-MS Homo sapiens
41 HDAC3 8841
Affinity Capture-MS Homo sapiens
42 B3GAT1  
Proximity Label-MS Homo sapiens
43 ERF  
Affinity Capture-MS Homo sapiens
44 TNK1  
Affinity Capture-MS Homo sapiens
45 B3GNT2 10678
Affinity Capture-MS Homo sapiens
46 IMPDH2 3615
Affinity Capture-MS Homo sapiens
47 USO1 8615
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
48 DYRK1A 1859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 KLF8  
Affinity Capture-MS Homo sapiens
50 MCM2 4171
Affinity Capture-MS Homo sapiens
51 HELZ 9931
Affinity Capture-MS Homo sapiens
52 FAM83B  
Affinity Capture-MS Homo sapiens
53 AP2A1 160
Affinity Capture-MS Homo sapiens
54 CCAR2 57805
Affinity Capture-MS Homo sapiens
55 AMOT 154796
Proximity Label-MS Homo sapiens
56 SEC24C 9632
Affinity Capture-MS Homo sapiens
57 RPN1 6184
Proximity Label-MS Homo sapiens
58 FBXW7  
Affinity Capture-MS Homo sapiens
59 PTPN23 25930
Proximity Label-MS Homo sapiens
60 IQGAP1 8826
Affinity Capture-MS Homo sapiens
61 CPSF7 79869
Affinity Capture-MS Homo sapiens
62 KLF6  
Proximity Label-MS Homo sapiens
63 ETV4  
Proximity Label-MS Homo sapiens
64 SSR1 6745
Proximity Label-MS Homo sapiens
65 PPEF1  
Affinity Capture-MS Homo sapiens
66 Cenpe  
Affinity Capture-MS Mus musculus
67 CLPB 81570
Affinity Capture-MS Homo sapiens
68 RPN2 6185
Proximity Label-MS Homo sapiens
69 STX6 10228
Proximity Label-MS Homo sapiens
70 AURKA 6790
Affinity Capture-MS Homo sapiens
71 SLC25A46 91137
Proximity Label-MS Homo sapiens
72 BCOR  
Proximity Label-MS Homo sapiens
73 DNAJC7 7266
Affinity Capture-MS Homo sapiens
74 GTSE1 51512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 SEC23A 10484
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
77 MAPRE1 22919
Affinity Capture-MS Homo sapiens
78 KLF15  
Proximity Label-MS Homo sapiens
79 EBAG9 9166
Proximity Label-MS Homo sapiens
80 FAM120A 23196
Affinity Capture-MS Homo sapiens
81 Lima1  
Affinity Capture-MS Mus musculus
82 DCP1B  
Affinity Capture-MS Homo sapiens
83 KIF14 9928
Affinity Capture-MS Homo sapiens
84 IMPDH1 3614
Affinity Capture-MS Homo sapiens
85 TRIM31  
Affinity Capture-MS Homo sapiens
86 GOLGA2 2801
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 KLF16  
Affinity Capture-MS Homo sapiens
88 PFN1 5216
Proximity Label-MS Homo sapiens
89 Fam83d  
Affinity Capture-MS Mus musculus
90 KRT8 3856
Proximity Label-MS Homo sapiens
91 KIAA1217 56243
Affinity Capture-MS Homo sapiens
92 PKP3 11187
Affinity Capture-MS Homo sapiens
93 MYO18A 399687
Affinity Capture-MS Homo sapiens
94 FOXA1  
Affinity Capture-MS Homo sapiens
95 ARHGEF39 84904
Affinity Capture-MS Homo sapiens
96 RPA3 6119
Proximity Label-MS Homo sapiens
97 EMD 2010
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
98 ECT2 1894
Affinity Capture-MS Homo sapiens
99 RB1CC1 9821
Affinity Capture-MS Homo sapiens
100 CTBP1 1487
Proximity Label-MS Homo sapiens
101 ANKHD1 54882
Affinity Capture-MS Homo sapiens
102 NCOR2  
Affinity Capture-MS Homo sapiens
103 RBM4 5936
Affinity Capture-MS Homo sapiens
104 Gorasp1  
Affinity Capture-MS Mus musculus
105 Sec24c  
Affinity Capture-MS Mus musculus
106 CLTCL1 8218
Affinity Capture-MS Homo sapiens
107 AHNAK 79026
Co-fractionation Homo sapiens
108 IRAK1  
Affinity Capture-MS Homo sapiens
109 TRIM37  
Proximity Label-MS Homo sapiens
110 PFDN2 5202
Affinity Capture-MS Homo sapiens
111 XPNPEP3 63929
Affinity Capture-MS Homo sapiens
112 CDKN2AIPNL  
Affinity Capture-MS Homo sapiens
113 VCL 7414
Affinity Capture-MS Homo sapiens
114 BTF3 689
Affinity Capture-MS Homo sapiens
115 MYH9 4627
Affinity Capture-MS Homo sapiens
116 VCP 7415
Affinity Capture-MS Homo sapiens
117 NTRK1 4914
Affinity Capture-MS Homo sapiens
118 BTBD3  
Affinity Capture-MS Homo sapiens
119 REL 5966
Co-fractionation Homo sapiens
120 DTX2 113878
Proximity Label-MS Homo sapiens
121 IQCB1  
Affinity Capture-MS Homo sapiens
122 DCTN1 1639
Affinity Capture-MS Homo sapiens
123 RAB7A 7879
Proximity Label-MS Homo sapiens
124 BMP2K 55589
Affinity Capture-MS Homo sapiens
125 TRIM66  
Affinity Capture-MS Homo sapiens
126 Hnrnpf 98758
Affinity Capture-MS Mus musculus
127 CSNK1E 1454
Affinity Capture-MS Homo sapiens
128 STIL  
Proximity Label-MS Homo sapiens
129 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
130 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
131 NFKB2 4791
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
132 Cdk5rap2  
Affinity Capture-MS Mus musculus
133 LATS2 26524
Proximity Label-MS Homo sapiens
134 PICALM 8301
Affinity Capture-MS Homo sapiens
135 ELOVL5 60481
Proximity Label-MS Homo sapiens
136 Ksr1  
Affinity Capture-MS Mus musculus
137 SEC61B 10952
Proximity Label-MS Homo sapiens
138 NFATC4 4776
Proximity Label-MS Homo sapiens
139 WDR33 55339
Affinity Capture-MS Homo sapiens
140 HNRNPH3 3189
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
141 KDF1  
Affinity Capture-MS Homo sapiens
142 P3H1 64175
Affinity Capture-MS Homo sapiens
143 CBFB 865
Affinity Capture-MS Homo sapiens
144 ANLN 54443
Affinity Capture-MS Homo sapiens
145 PHLPP1  
Proximity Label-MS Homo sapiens
146 LIMA1 51474
Affinity Capture-MS Homo sapiens
147 TMPO 7112
Affinity Capture-MS Homo sapiens
148 NUP155 9631
Proximity Label-MS Homo sapiens
149 ARHGAP32  
Affinity Capture-MS Homo sapiens
150 SMG7  
Affinity Capture-MS Homo sapiens
151 MAP7 9053
Affinity Capture-MS Homo sapiens
152 CLINT1 9685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 Myh9 17886
Affinity Capture-MS Mus musculus
154 SKP2  
Affinity Capture-MS Homo sapiens
155 PXN 5829
Proximity Label-MS Homo sapiens
156 AMBRA1  
Affinity Capture-MS Homo sapiens
157 XPO1 7514
Affinity Capture-MS Homo sapiens
158 KANK2 25959
Affinity Capture-MS Homo sapiens
159 RC3H2  
Affinity Capture-MS Homo sapiens
160 SEC13 6396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 RELA 5970
Co-fractionation Homo sapiens
162 SIRT7  
Affinity Capture-MS Homo sapiens
163 CTNNB1 1499
Affinity Capture-MS Homo sapiens
164 C4BPA 722
Affinity Capture-MS Homo sapiens
165 MEX3A  
Affinity Capture-RNA Homo sapiens
166 CD274 29126
Affinity Capture-MS Homo sapiens
167 BCAP31 10134
Proximity Label-MS Homo sapiens
168 HSD3B7 80270
Proximity Label-MS Homo sapiens
169 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 PUM1 9698
Affinity Capture-MS Homo sapiens
171 STIM1 6786
Proximity Label-MS Homo sapiens
172 EGFR 1956
Affinity Capture-MS Homo sapiens
173 MYO19  
Affinity Capture-MS Homo sapiens
174 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
175 AKAP8L  
Affinity Capture-MS Homo sapiens
176 DUSP16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 RIPK4  
Affinity Capture-MS Homo sapiens
178 SASS6 163786
Proximity Label-MS Homo sapiens
179 SEC24A 10802
Affinity Capture-MS Homo sapiens
180 ELMSAN1  
Affinity Capture-MS Homo sapiens
181 FUBP3 8939
Affinity Capture-MS Homo sapiens
182 AURKB 9212
Affinity Capture-MS Homo sapiens
183 CDK2 1017
Affinity Capture-MS Homo sapiens
184 CEP250 11190
Affinity Capture-MS Homo sapiens
185 SYNPO 11346
Affinity Capture-MS Homo sapiens
186 MYO6 4646
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
187 Uso1 56041
Affinity Capture-MS Mus musculus
188 ATP2A1 487
Proximity Label-MS Homo sapiens
189 SAV1  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
190 RAB9A 9367
Proximity Label-MS Homo sapiens
191 GOLGA1  
Proximity Label-MS Homo sapiens
192 RPA4  
Proximity Label-MS Homo sapiens
193 TRAF2 7186
Affinity Capture-Western Homo sapiens
194 CRY2  
Affinity Capture-MS Homo sapiens
195 FKBP3 2287
Co-fractionation Homo sapiens
196 RNF2  
Affinity Capture-MS Homo sapiens
197 Kctd5  
Affinity Capture-MS Mus musculus
198 CLTC 1213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
199 NUP35 129401
Proximity Label-MS Homo sapiens
200 STAMBPL1  
Affinity Capture-MS Homo sapiens
201 2510003E04Rik  
Affinity Capture-MS Mus musculus
202 TRAF3 7187
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
203 DOCK3  
Affinity Capture-MS Homo sapiens
204 CCDC8  
Affinity Capture-MS Homo sapiens
205 RAB5A 5868
Proximity Label-MS Homo sapiens
206 DSP 1832
Affinity Capture-MS Homo sapiens
207 STAT4  
Proximity Label-MS Homo sapiens
208 SEC24B 10427
Affinity Capture-MS Homo sapiens
209 KRT18 3875
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
210 RBM14 10432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
211 RNF43  
Proximity Label-MS Homo sapiens
212 UBE3B 89910
Affinity Capture-MS Homo sapiens
213 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
214 FASN 2194
Negative Genetic Homo sapiens
215 TERF2  
Affinity Capture-MS Homo sapiens
216 SIRT6  
Affinity Capture-MS Homo sapiens
217 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
218 TRPS1  
Affinity Capture-MS Homo sapiens
219 WDR77 79084
Affinity Capture-MS Homo sapiens
220 Akap8  
Affinity Capture-MS Mus musculus
221 PPP1R13L 10848
Affinity Capture-MS Homo sapiens
222 SEC23B 10483
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
223 ATXN2 6311
Affinity Capture-MS Homo sapiens
224 Myo1c 17913
Affinity Capture-MS Mus musculus
225 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
226 PDHA1 5160
Affinity Capture-MS Homo sapiens
227 IKBKB 3551
Co-fractionation Homo sapiens
228 HNRNPM 4670
Affinity Capture-MS Homo sapiens
229 CAPZA2 830
Affinity Capture-MS Homo sapiens
230 SQSTM1 8878
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
231 RBM39 9584
Affinity Capture-MS Homo sapiens
232 DCTN2 10540
Proximity Label-MS Homo sapiens
233 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
234 FAM83H 286077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
235 ERGIC1 57222
Proximity Label-MS Homo sapiens
236 PRRC2B  
Affinity Capture-MS Homo sapiens
237 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
238 HNRNPK 3190
Affinity Capture-MS Homo sapiens
239 LATS1  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
240 FAM120C 54954
Affinity Capture-MS Homo sapiens
241 COMMD3-BMI1 100532731
Affinity Capture-MS Homo sapiens
242 KLF5  
Proximity Label-MS Homo sapiens
243 PXMP2  
Proximity Label-MS Homo sapiens
244 TBK1 29110
Affinity Capture-Western Homo sapiens
245 ERGIC2 51290
Proximity Label-MS Homo sapiens
246 TERF1 7013
Affinity Capture-MS Homo sapiens
247 CHMP4C 92421
Affinity Capture-MS Homo sapiens
248 SEC62 7095
Proximity Label-MS Homo sapiens
249 GAK 2580
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
250 CAD 790
Affinity Capture-MS Homo sapiens
251 FAM83A  
Affinity Capture-MS Homo sapiens
252 CEP135  
Proximity Label-MS Homo sapiens
253 EEF1D 1936
Affinity Capture-MS Homo sapiens
254 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
255 PSMF1 9491
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
256 WDR92 116143
Affinity Capture-MS Homo sapiens
257 SPG20 23111
Affinity Capture-MS Homo sapiens
258 EPHA2 1969
Proximity Label-MS Homo sapiens
259 PIH1D1  
Affinity Capture-MS Homo sapiens
260 NEFL 4747
Affinity Capture-MS Homo sapiens
261 C9orf72  
Affinity Capture-MS Homo sapiens
262 AR 367
Affinity Capture-MS Homo sapiens
263 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
264 TRIM29 23650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
265 KLHL12  
Affinity Capture-MS Homo sapiens
266 PRMT1 3276
Affinity Capture-MS Homo sapiens
267 ARX  
Affinity Capture-MS Homo sapiens
268 CUL4A 8451
Affinity Capture-MS Homo sapiens
269 DERL1 79139
Proximity Label-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here