Gene description for HIPK3
Gene name homeodomain interacting protein kinase 3
Gene symbol HIPK3
Other names/aliases DYRK6
FIST3
PKY
YAK1
Species Homo sapiens
 Database cross references - HIPK3
ExoCarta ExoCarta_10114
Vesiclepedia VP_10114
Entrez Gene 10114
HGNC 4915
MIM 604424
UniProt Q9H422  
 HIPK3 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for HIPK3
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein tyrosine kinase activity GO:0004713 IBA
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    apoptotic process GO:0006915 IEA
    mRNA transcription GO:0009299 IDA
    peptidyl-serine phosphorylation GO:0018105 ISS
    peptidyl-threonine phosphorylation GO:0018107 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of JUN kinase activity GO:0043508 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    nuclear body GO:0016604 IDA
    PML body GO:0016605 IBA
 Experiment description of studies that identified HIPK3 in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for HIPK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
2 HNRNPH1 3187
Protein-RNA Homo sapiens
3 ARRB2 409
Two-hybrid Homo sapiens
4 FADD 8772
Affinity Capture-Western Homo sapiens
5 GRB2 2885
Two-hybrid Homo sapiens
6 SIAH2 6478
Two-hybrid Homo sapiens
7 TP53 7157
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 FAS 355
Affinity Capture-Western Homo sapiens
9 ZYX 7791
Two-hybrid Homo sapiens
10 C1QA 712
Two-hybrid Homo sapiens
11 SRSF1 6426
Protein-RNA Homo sapiens
12 HNRNPA2B1 3181
Protein-RNA Homo sapiens
13 LIMS2 55679
Two-hybrid Homo sapiens
14 SRSF5 6430
Protein-RNA Homo sapiens
15 HNRNPA1 3178
Protein-RNA Homo sapiens
16 TGFB1I1 7041
Two-hybrid Homo sapiens
17 TRA2B 6434
Protein-RNA Homo sapiens
18 CREB1  
Two-hybrid Homo sapiens
19 TP73  
Two-hybrid Homo sapiens
20 GORASP1 64689
Two-hybrid Homo sapiens
21 UBE2I 7329
Two-hybrid Homo sapiens
22 HEYL  
Two-hybrid Homo sapiens
23 GLUL 2752
Two-hybrid Homo sapiens
24 TOX4  
Two-hybrid Homo sapiens
25 LIMD1 8994
Two-hybrid Homo sapiens
26 NR2F2  
Two-hybrid Homo sapiens
27 ZNF107  
Two-hybrid Homo sapiens
28 LIMK2 3985
Two-hybrid Homo sapiens
29 MTMR4  
Two-hybrid Homo sapiens
30 SUMO1 7341
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which HIPK3 is involved
No pathways found





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