Gene description for LIMD1
Gene name LIM domains containing 1
Gene symbol LIMD1
Other names/aliases -
Species Homo sapiens
 Database cross references - LIMD1
ExoCarta ExoCarta_8994
Vesiclepedia VP_8994
Entrez Gene 8994
HGNC 6612
MIM 604543
UniProt Q9UGP4  
 LIMD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for LIMD1
Molecular Function
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 ISS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    response to hypoxia GO:0001666 IBA
    response to hypoxia GO:0001666 IDA
    osteoblast development GO:0002076 ISS
    regulation of DNA-templated transcription GO:0006355 IBA
    cytoskeleton organization GO:0007010 IBA
    cytoskeleton organization GO:0007010 IMP
    regulation of cell shape GO:0008360 IMP
    phosphorylation GO:0016310 IDA
    cell migration GO:0016477 IMP
    P-body assembly GO:0033962 IMP
    miRNA-mediated post-transcriptional gene silencing GO:0035195 IMP
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 IMP
    negative regulation of hippo signaling GO:0035331 IBA
    negative regulation of hippo signaling GO:0035331 IDA
    negative regulation of osteoblast differentiation GO:0045668 ISS
    negative regulation of DNA-templated transcription GO:0045892 IDA
    negative regulation of DNA-templated transcription GO:0045892 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISS
Subcellular Localization
    P-body GO:0000932 IBA
    P-body GO:0000932 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    transcription regulator complex GO:0005667 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 IDA
    focal adhesion GO:0005925 IDA
    RISC complex GO:0016442 IDA
 Experiment description of studies that identified LIMD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LIMD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 LGALS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
5 ETV6  
Proximity Label-MS Homo sapiens
6 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
7 TRIM35 23087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DISC1 27185
Affinity Capture-MS Homo sapiens
9 C9orf78 51759
Co-fractionation Homo sapiens
10 EGLN1 54583
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 VHL  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
13 TEKT3 64518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LMO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RFPL4B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PHYHIP 9796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ZNF843  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ACTR3 10096
Proximity Label-MS Homo sapiens
19 ZFYVE1 53349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PLCG1 5335
Co-fractionation Homo sapiens
21 TULP3 7289
Affinity Capture-MS Homo sapiens
22 TEX19  
Affinity Capture-MS Homo sapiens
23 GOLGA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ZNF576  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 EHD1 10938
Co-fractionation Homo sapiens
27 NINL  
Proximity Label-MS Homo sapiens
28 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PRPH 5630
Proximity Label-MS Homo sapiens
30 PHAX  
Affinity Capture-MS Homo sapiens
31 CDPF1  
Affinity Capture-MS Homo sapiens
32 PFDN2 5202
Affinity Capture-MS Homo sapiens
33 RNF43  
Proximity Label-MS Homo sapiens
34 PEX14 5195
Proximity Label-MS Homo sapiens
35 BICD2 23299
Proximity Label-MS Homo sapiens
36 RPA4  
Proximity Label-MS Homo sapiens
37 ACTB 60
Proximity Label-MS Homo sapiens
38 Ppp2r3a  
Affinity Capture-MS Mus musculus
39 STIL  
Proximity Label-MS Homo sapiens
40 TANC2  
Affinity Capture-MS Homo sapiens
41 LATS2 26524
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
42 TMPRSS13  
Affinity Capture-MS Homo sapiens
43 IGBP1 3476
Co-fractionation Homo sapiens
44 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
45 PPP2R3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MAP3K6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C1orf105  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CEP135  
Proximity Label-MS Homo sapiens
49 XPO1 7514
Affinity Capture-MS Homo sapiens
50 EGLN3  
Affinity Capture-Western Homo sapiens
51 BAG2 9532
Affinity Capture-MS Homo sapiens
52 STAC  
Two-hybrid Homo sapiens
53 CTNNB1 1499
Proximity Label-MS Homo sapiens
54 TRAF6 7189
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
56 UBR7  
Co-fractionation Homo sapiens
57 EIF4E 1977
Co-fractionation Homo sapiens
58 GTF3C4 9329
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
59 ABLIM3 22885
Affinity Capture-MS Homo sapiens
60 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RPA3 6119
Proximity Label-MS Homo sapiens
62 NIN 51199
Proximity Label-MS Homo sapiens
63 HSPA1A 3303
Affinity Capture-MS Homo sapiens
64 DSTN 11034
Co-fractionation Homo sapiens
65 BICD1 636
Proximity Label-MS Homo sapiens
66 AMOT 154796
Proximity Label-MS Homo sapiens
67 CTNNA1 1495
Proximity Label-MS Homo sapiens
68 ARHGAP31 57514
Affinity Capture-MS Homo sapiens
69 APBA1  
Affinity Capture-MS Homo sapiens
70 DZIP3  
Affinity Capture-MS Homo sapiens
71 TRIP6 7205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 PDLIM7 9260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
74 RARA 5914
Affinity Capture-Western Homo sapiens
75 RHOBTB3 22836
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 FBXO10  
Affinity Capture-MS Homo sapiens
77 HIPK3 10114
Two-hybrid Homo sapiens
78 AJUBA  
Affinity Capture-MS Homo sapiens
79 Arhgap30  
Affinity Capture-MS Mus musculus
80 FKBP9 11328
Co-fractionation Homo sapiens
81 LATS1  
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
82 EGLN2  
Affinity Capture-Western Homo sapiens
83 KRT8 3856
Proximity Label-MS Homo sapiens
84 DDX6 1656
Co-fractionation Homo sapiens
85 SLC27A3 11000
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 PYGB 5834
Co-fractionation Homo sapiens
87 PXN 5829
Proximity Label-MS Homo sapiens
88 LIMD1 8994
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
89 UBA6 55236
Co-fractionation Homo sapiens
View the network image/svg+xml



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