Gene description for ARHGAP31
Gene name Rho GTPase activating protein 31
Gene symbol ARHGAP31
Other names/aliases AOS1
CDGAP
Species Homo sapiens
 Database cross references - ARHGAP31
ExoCarta ExoCarta_57514
Vesiclepedia VP_57514
Entrez Gene 57514
HGNC 29216
MIM 610911
UniProt Q2M1Z3  
 ARHGAP31 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGAP31
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IEA
    lamellipodium GO:0030027 IBA
 Experiment description of studies that identified ARHGAP31 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGAP31
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUP93 9688
Affinity Capture-MS Homo sapiens
2 HACD3 51495
Affinity Capture-MS Homo sapiens
3 SF3B5 83443
Affinity Capture-MS Homo sapiens
4 ARL1 400
Affinity Capture-MS Homo sapiens
5 ITSN1 6453
Affinity Capture-Western Homo sapiens
6 HMGB1 3146
Affinity Capture-MS Homo sapiens
7 PCNA 5111
Affinity Capture-MS Homo sapiens
8 XPO1 7514
Affinity Capture-MS Homo sapiens
9 NME1-NME2 654364
Affinity Capture-MS Homo sapiens
10 SLC25A22  
Affinity Capture-MS Homo sapiens
11 RHOA 387
FRET Homo sapiens
12 RIF1  
Affinity Capture-MS Homo sapiens
13 Arhgap28  
Affinity Capture-MS Mus musculus
14 PRDX5 25824
Affinity Capture-MS Homo sapiens
15 CHCHD2  
Affinity Capture-MS Homo sapiens
16 MRPL10 124995
Affinity Capture-MS Homo sapiens
17 TMEM263 90488
Affinity Capture-MS Homo sapiens
18 CFL1 1072
Affinity Capture-MS Homo sapiens
19 SNRPF 6636
Affinity Capture-MS Homo sapiens
20 ARF4 378
Affinity Capture-MS Homo sapiens
21 TUBB4A 10382
Affinity Capture-MS Homo sapiens
22 MSI1  
Affinity Capture-MS Homo sapiens
23 MCAM 4162
Proximity Label-MS Homo sapiens
24 CDC42 998
FRET Homo sapiens
25 TRIM37  
Proximity Label-MS Homo sapiens
26 PRDX3 10935
Affinity Capture-MS Homo sapiens
27 PRDX2 7001
Affinity Capture-MS Homo sapiens
28 DHRS7B 25979
Affinity Capture-MS Homo sapiens
29 TLN1 7094
Affinity Capture-MS Homo sapiens
30 ARF1 375
Affinity Capture-MS Homo sapiens
31 LIMD1 8994
Affinity Capture-MS Homo sapiens
32 TIMM23 100287932
Affinity Capture-MS Homo sapiens
33 Arhgap18  
Affinity Capture-MS Mus musculus
34 KRAS 3845
Proximity Label-MS Homo sapiens
35 ATP5C1 509
Affinity Capture-MS Homo sapiens
36 RAC1 5879
Two-hybrid Homo sapiens
FRET Homo sapiens
37 MRPS17 51373
Affinity Capture-MS Homo sapiens
38 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
39 CSTB 1476
Affinity Capture-MS Homo sapiens
40 BOLA2 552900
Affinity Capture-MS Homo sapiens
41 CYLD  
Affinity Capture-MS Homo sapiens
42 PPP2CA 5515
Affinity Capture-MS Homo sapiens
43 PPIF 10105
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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