Gene description for GTF3C4
Gene name general transcription factor IIIC, polypeptide 4, 90kDa
Gene symbol GTF3C4
Other names/aliases KAT12
TFIII90
TFIIIC290
TFIIIC90
TFIIICDELTA
Species Homo sapiens
 Database cross references - GTF3C4
ExoCarta ExoCarta_9329
Vesiclepedia VP_9329
Entrez Gene 9329
HGNC 4667
MIM 604892
UniProt Q9UKN8  
 GTF3C4 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
 Gene ontology annotations for GTF3C4
Molecular Function
    RNA polymerase III general transcription initiation factor activity GO:0000995 IDA
    DNA binding GO:0003677 IC
    protein binding GO:0005515 IPI
    histone H3K56 acetyltransferase activity GO:0032931 IEA
    histone H3K14 acetyltransferase activity GO:0036408 IDA
    histone H3K9 acetyltransferase activity GO:0043992 IEA
    histone H3K18 acetyltransferase activity GO:0043993 IEA
    histone H3K23 acetyltransferase activity GO:0043994 IEA
    histone H4K5 acetyltransferase activity GO:0043995 IEA
    histone H4K8 acetyltransferase activity GO:0043996 IEA
    histone H4K12 acetyltransferase activity GO:0043997 IEA
    histone H2AK5 acetyltransferase activity GO:0043999 IEA
    histone H2AK9 acetyltransferase activity GO:0044012 IEA
    histone H2BK5 acetyltransferase activity GO:0044014 IEA
    histone H2BK12 acetyltransferase activity GO:0044015 IEA
    histone H3K4 acetyltransferase activity GO:0044016 IEA
    histone H3K27 acetyltransferase activity GO:0044017 IEA
    histone H3K36 acetyltransferase activity GO:0044018 IEA
    histone H4K16 acetyltransferase activity GO:0046972 IEA
    histone H3K122 acetyltransferase activity GO:0140908 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    transcription by RNA polymerase III GO:0006383 IC
    transcription initiation at RNA polymerase III promoter GO:0006384 IEA
    5S class rRNA transcription by RNA polymerase III GO:0042791 IC
    tRNA transcription by RNA polymerase III GO:0042797 IC
Subcellular Localization
    transcription factor TFIIIC complex GO:0000127 IBA
    transcription factor TFIIIC complex GO:0000127 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified GTF3C4 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for GTF3C4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMC3 9126
Affinity Capture-MS Homo sapiens
2 SENP3 26168
Affinity Capture-MS Homo sapiens
3 CBX1 10951
Affinity Capture-MS Homo sapiens
4 COIL  
Proximity Label-MS Homo sapiens
5 Gtf3c2  
Affinity Capture-MS Mus musculus
6 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
7 ACTC1 70
Proximity Label-MS Homo sapiens
8 NOP56 10528
Affinity Capture-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 CLIP2 7461
Affinity Capture-MS Homo sapiens
11 MID1 4281
Affinity Capture-MS Homo sapiens
12 SPAG9 9043
Affinity Capture-MS Homo sapiens
13 CENPA  
Proximity Label-MS Homo sapiens
14 USP36  
Affinity Capture-MS Homo sapiens
15 DPPA4  
Affinity Capture-MS Homo sapiens
16 MCM2 4171
Affinity Capture-MS Homo sapiens
17 ZNF638 27332
Affinity Capture-MS Homo sapiens
18 METTL22  
Affinity Capture-MS Homo sapiens
19 HIST2H3A 333932
Reconstituted Complex Homo sapiens
20 NPM1 4869
Affinity Capture-MS Homo sapiens
21 POU5F1  
Affinity Capture-MS Homo sapiens
22 HEXB 3074
Co-fractionation Homo sapiens
23 MCM6 4175
Affinity Capture-MS Homo sapiens
24 PLCG1 5335
Co-fractionation Homo sapiens
25 HELLS 3070
Affinity Capture-MS Homo sapiens
26 GTF3C5 9328
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 EED  
Affinity Capture-MS Homo sapiens
28 ZNF644  
Affinity Capture-MS Homo sapiens
29 PPP6R1 22870
Affinity Capture-MS Homo sapiens
30 MYCN  
Affinity Capture-MS Homo sapiens
31 HK1 3098
Co-fractionation Homo sapiens
32 RAD23A 5886
Co-fractionation Homo sapiens
33 RBPJ 3516
Affinity Capture-MS Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 AURKA 6790
Reconstituted Complex Homo sapiens
36 USP15 9958
Affinity Capture-MS Homo sapiens
37 NOL3 8996
Co-fractionation Homo sapiens
38 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
39 POLR3F  
Reconstituted Complex Homo sapiens
40 PARK2  
Affinity Capture-MS Homo sapiens
41 EPS15L1 58513
Affinity Capture-MS Homo sapiens
42 TUFM 7284
Co-fractionation Homo sapiens
43 CBX3 11335
Proximity Label-MS Homo sapiens
44 BARD1 580
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 METTL21C  
Affinity Capture-MS Homo sapiens
47 FAIM  
Affinity Capture-MS Homo sapiens
48 IFI16 3428
Affinity Capture-MS Homo sapiens
49 GTF3C2  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 PLK1 5347
Affinity Capture-MS Homo sapiens
51 Bag2  
Affinity Capture-MS Mus musculus
52 SMC1A 8243
Affinity Capture-MS Homo sapiens
53 HECTD1 25831
Affinity Capture-MS Homo sapiens
54 FOXS1  
Affinity Capture-MS Homo sapiens
55 PAPSS2 9060
Co-fractionation Homo sapiens
56 BRCA1 672
Two-hybrid Homo sapiens
57 CD3EAP  
Proximity Label-MS Homo sapiens
58 SCARNA22  
Affinity Capture-RNA Homo sapiens
59 ZNF747  
Affinity Capture-MS Homo sapiens
60 GTF3C3 9330
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
61 HSF1  
Affinity Capture-MS Homo sapiens
62 RAD1  
Affinity Capture-MS Homo sapiens
63 SMARCAD1  
Affinity Capture-MS Homo sapiens
64 LMNB1 4001
Proximity Label-MS Homo sapiens
65 GTF3C1  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
66 MEX3A  
Affinity Capture-RNA Homo sapiens
67 CUL3 8452
Affinity Capture-MS Homo sapiens
68 EGFR 1956
Negative Genetic Homo sapiens
69 ZC3HC1  
Co-fractionation Homo sapiens
70 MCM4 4173
Affinity Capture-MS Homo sapiens
71 FOXL1  
Affinity Capture-MS Homo sapiens
72 PDCD11 22984
Affinity Capture-MS Homo sapiens
73 HEXA 3073
Co-fractionation Homo sapiens
74 TBP  
Reconstituted Complex Homo sapiens
75 GTF3C6  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
76 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 USP37  
Proximity Label-MS Homo sapiens
78 TOP1 7150
Co-fractionation Homo sapiens
79 SBNO1 55206
Affinity Capture-MS Homo sapiens
80 RPL22 6146
Co-fractionation Homo sapiens
81 DDRGK1 65992
Affinity Capture-MS Homo sapiens
82 RNF2  
Affinity Capture-MS Homo sapiens
83 FOXQ1  
Affinity Capture-MS Homo sapiens
84 Rpl35 66489
Affinity Capture-MS Mus musculus
85 MSN 4478
Co-fractionation Homo sapiens
86 NANOG  
Affinity Capture-MS Homo sapiens
87 PABPC3 5042
Affinity Capture-MS Homo sapiens
88 NFATC2  
Affinity Capture-MS Homo sapiens
89 PASK  
Affinity Capture-MS Homo sapiens
90 HUWE1 10075
Affinity Capture-MS Homo sapiens
91 RPS16 6217
Affinity Capture-MS Homo sapiens
92 PROSC 11212
Co-fractionation Homo sapiens
93 PFAS 5198
Affinity Capture-MS Homo sapiens
94 Gtf3c6  
Affinity Capture-MS Mus musculus
95 EHD1 10938
Co-fractionation Homo sapiens
96 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
97 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 SIRT6  
Affinity Capture-MS Homo sapiens
99 FBXL6  
Affinity Capture-MS Homo sapiens
100 TP53 7157
Affinity Capture-MS Homo sapiens
101 EHD4 30844
Co-fractionation Homo sapiens
102 MTMR3  
Affinity Capture-MS Homo sapiens
103 FOXJ2  
Affinity Capture-MS Homo sapiens
104 POLR3C 10623
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
105 FOXB1  
Affinity Capture-MS Homo sapiens
106 IVNS1ABP  
Affinity Capture-MS Homo sapiens
107 EZR 7430
Co-fractionation Homo sapiens
108 Gtf3c3  
Affinity Capture-MS Mus musculus
109 FKBP9 11328
Co-fractionation Homo sapiens
110 ZNF764 92595
Affinity Capture-MS Homo sapiens
111 HNRNPK 3190
Co-fractionation Homo sapiens
112 UFL1 23376
Affinity Capture-MS Homo sapiens
113 API5 8539
Co-fractionation Homo sapiens
114 ZNF688  
Affinity Capture-MS Homo sapiens
115 C16orf72 29035
Affinity Capture-MS Homo sapiens
116 GAPVD1 26130
Affinity Capture-MS Homo sapiens
117 CBX8 57332
Affinity Capture-MS Homo sapiens
118 CIT 11113
Affinity Capture-MS Homo sapiens
119 METTL3  
Affinity Capture-MS Homo sapiens
120 LIMD1 8994
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
121 FOXM1  
Affinity Capture-MS Homo sapiens
122 ANK3  
Affinity Capture-MS Homo sapiens
123 C9orf72  
Affinity Capture-MS Homo sapiens
124 AR 367
Proximity Label-MS Homo sapiens
125 PTRF 284119
Co-fractionation Homo sapiens
126 WARS 7453
Co-fractionation Homo sapiens
127 XPNPEP1 7511
Co-fractionation Homo sapiens
128 PSME4 23198
Affinity Capture-MS Homo sapiens
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