Gene description for PLXNC1
Gene name plexin C1
Gene symbol PLXNC1
Other names/aliases CD232
PLXN-C1
VESPR
Species Homo sapiens
 Database cross references - PLXNC1
ExoCarta ExoCarta_10154
Vesiclepedia VP_10154
Entrez Gene 10154
HGNC 9106
MIM 604259
UniProt O60486  
 PLXNC1 identified in sEVs derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for PLXNC1
Molecular Function
    signaling receptor binding GO:0005102 TAS
    protein binding GO:0005515 IPI
    semaphorin receptor activity GO:0017154 IBA
Biological Process
    cell adhesion GO:0007155 TAS
    negative regulation of cell adhesion GO:0007162 IBA
    synapse assembly GO:0007416 IBA
    regulation of cell shape GO:0008360 IBA
    regulation of cell migration GO:0030334 IBA
    positive regulation of axonogenesis GO:0050772 IBA
    semaphorin-plexin signaling pathway GO:0071526 IBA
    regulation of synapse pruning GO:1905806 IEA
Subcellular Localization
    semaphorin receptor complex GO:0002116 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    cerebellar climbing fiber to Purkinje cell synapse GO:0150053 IEA
 Experiment description of studies that identified PLXNC1 in sEVs
1
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PLXNC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA5 3309
Affinity Capture-MS Homo sapiens
2 POTEF 728378
Affinity Capture-MS Homo sapiens
3 FBXO2 26232
Affinity Capture-MS Homo sapiens
4 MANEA 79694
Affinity Capture-MS Homo sapiens
5 FAM163B  
Affinity Capture-MS Homo sapiens
6 PDC  
Affinity Capture-MS Homo sapiens
7 HLA-DPA1  
Affinity Capture-MS Homo sapiens
8 CEMP1  
Affinity Capture-MS Homo sapiens
9 GDF11 10220
Affinity Capture-MS Homo sapiens
10 TMEM205 374882
Affinity Capture-MS Homo sapiens
11 LHX3  
Affinity Capture-MS Homo sapiens
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