Gene description for BTN3A3
Gene name butyrophilin, subfamily 3, member A3
Gene symbol BTN3A3
Other names/aliases BTF3
BTN3.3
Species Homo sapiens
 Database cross references - BTN3A3
ExoCarta ExoCarta_10384
Vesiclepedia VP_10384
Entrez Gene 10384
HGNC 1140
MIM 613595
UniProt O00478  
 BTN3A3 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for BTN3A3
Molecular Function
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
Biological Process
    regulation of cytokine production GO:0001817 IBA
    T cell mediated immunity GO:0002456 IMP
    T cell receptor signaling pathway GO:0050852 IBA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified BTN3A3 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for BTN3A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPY19L1 23333
Affinity Capture-MS Homo sapiens
2 UFSP2 55325
Affinity Capture-MS Homo sapiens
3 HSD17B11 51170
Proximity Label-MS Homo sapiens
4 TOMM40 10452
Affinity Capture-MS Homo sapiens
5 CTSL 1514
Two-hybrid Homo sapiens
6 RPN2 6185
Proximity Label-MS Homo sapiens
7 C1orf27 54953
Affinity Capture-MS Homo sapiens
8 METTL7A 25840
Proximity Label-MS Homo sapiens
9 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BTN3A2 11118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 EMD 2010
Proximity Label-MS Homo sapiens
12 TRIM68 55128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ARL8B 55207
Two-hybrid Homo sapiens
14 BTN3A1 11119
Affinity Capture-MS Homo sapiens
15 SEC62 7095
Proximity Label-MS Homo sapiens
16 CKAP5 9793
Affinity Capture-MS Homo sapiens
17 RAB13 5872
Affinity Capture-MS Homo sapiens
18 RAB11B 9230
Affinity Capture-MS Homo sapiens
19 EPHA2 1969
Proximity Label-MS Homo sapiens
20 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RPN1 6184
Proximity Label-MS Homo sapiens
22 SEC61B 10952
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which BTN3A3 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Butyrophilin (BTN) family interactions TAS Reactome
Immune System TAS Reactome





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