Gene description for Nckap1l
Gene name NCK associated protein 1 like
Gene symbol Nckap1l
Other names/aliases 4930568P13Rik
AI463083
Hem1
Hemp1
Species Mus musculus
 Database cross references - Nckap1l
ExoCarta ExoCarta_105855
Vesiclepedia VP_105855
Entrez Gene 105855
 Nckap1l identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nckap1l
Molecular Function
    GTPase activator activity GO:0005096 ISO
    GTPase activator activity GO:0005096 ISS
    protein kinase activator activity GO:0030295 ISO
    protein kinase activator activity GO:0030295 ISS
    protein-containing complex binding GO:0044877 ISO
    protein-containing complex binding GO:0044877 ISS
    TORC2 complex binding GO:1904841 ISO
    TORC2 complex binding GO:1904841 ISS
Biological Process
    cell morphogenesis GO:0000902 IBA
    B cell homeostasis GO:0001782 IMP
    myeloid cell homeostasis GO:0002262 IMP
    positive regulation of leukocyte migration GO:0002687 ISO
    positive regulation of leukocyte migration GO:0002687 ISS
    chemotaxis GO:0006935 ISO
    chemotaxis GO:0006935 ISS
    response to xenobiotic stimulus GO:0009410 ISO
    response to xenobiotic stimulus GO:0009410 ISS
    cell migration GO:0016477 IBA
    maintenance of cell polarity GO:0030011 ISO
    maintenance of cell polarity GO:0030011 ISS
    cell projection assembly GO:0030031 IBA
    neutrophil chemotaxis GO:0030593 ISO
    positive regulation of actin filament polymerization GO:0030838 IMP
    positive regulation of actin filament polymerization GO:0030838 ISO
    positive regulation of actin filament polymerization GO:0030838 ISS
    cortical actin cytoskeleton organization GO:0030866 IBA
    cortical actin cytoskeleton organization GO:0030866 IMP
    cortical actin cytoskeleton organization GO:0030866 ISO
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of B cell proliferation GO:0030890 ISS
    negative regulation of interleukin-17 production GO:0032700 IMP
    negative regulation of interleukin-6 production GO:0032715 IMP
    positive regulation of cell adhesion mediated by integrin GO:0033630 IMP
    erythrocyte homeostasis GO:0034101 IMP
    intracellular signal transduction GO:0035556 ISS
    positive regulation of T cell proliferation GO:0042102 IMP
    positive regulation of T cell proliferation GO:0042102 ISO
    positive regulation of T cell proliferation GO:0042102 ISS
    positive regulation of phosphorylation GO:0042327 ISO
    T cell homeostasis GO:0043029 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    negative regulation of cytotoxic T cell degranulation GO:0043318 ISO
    negative regulation of cytotoxic T cell degranulation GO:0043318 ISS
    positive regulation of CD4-positive, alpha-beta T cell differentiation GO:0043372 IMP
    positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043378 IMP
    positive regulation of B cell differentiation GO:0045579 IMP
    positive regulation of gamma-delta T cell differentiation GO:0045588 IMP
    positive regulation of lymphocyte differentiation GO:0045621 IMP
    positive regulation of erythrocyte differentiation GO:0045648 IMP
    neuron projection morphogenesis GO:0048812 IBA
    erythrocyte development GO:0048821 IEA
    B cell receptor signaling pathway GO:0050853 ISO
    B cell receptor signaling pathway GO:0050853 ISS
    positive regulation of phagocytosis, engulfment GO:0060100 IMP
    protein-containing complex assembly GO:0065003 IMP
    actin polymerization-dependent cell motility GO:0070358 ISO
    actin polymerization-dependent cell motility GO:0070358 ISS
    positive regulation of neutrophil chemotaxis GO:0090023 IMP
    positive regulation of neutrophil migration GO:1902624 ISO
    positive regulation of neutrophil migration GO:1902624 ISS
    positive regulation of TORC2 signaling GO:1904515 ISO
    positive regulation of TORC2 signaling GO:1904515 ISS
Subcellular Localization
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 IMP
    SCAR complex GO:0031209 ISO
 Experiment description of studies that identified Nckap1l in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nckap1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Foxp3  
Affinity Capture-MS Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Abi1 11308
Affinity Capture-MS Mus musculus
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