Gene description for Abi1
Gene name abl-interactor 1
Gene symbol Abi1
Other names/aliases E3B1
NAP1
Ssh3bp1
Species Mus musculus
 Database cross references - Abi1
ExoCarta ExoCarta_11308
Vesiclepedia VP_11308
Entrez Gene 11308
UniProt Q8CBW3  
 Abi1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Abi1
Molecular Function
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    protein tyrosine kinase activator activity GO:0030296 IDA
    signaling adaptor activity GO:0035591 IBA
    signaling adaptor activity GO:0035591 IDA
    signaling adaptor activity GO:0035591 ISO
Biological Process
    somitogenesis GO:0001756 IMP
    neuron migration GO:0001764 IBA
    megakaryocyte development GO:0035855 IMP
    dendrite morphogenesis GO:0048813 IDA
    cell projection morphogenesis GO:0048858 IBA
    positive regulation of protein tyrosine kinase activity GO:0061098 ISO
    lamellipodium morphogenesis GO:0072673 IMP
    postsynapse to nucleus signaling pathway GO:0099527 ISO
Subcellular Localization
    intracellular anatomical structure GO:0005622 ISO
    nucleus GO:0005634 IEA
    cytoskeleton GO:0005856 IEA
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    lamellipodium GO:0030027 IBA
    lamellipodium GO:0030027 IDA
    lamellipodium GO:0030027 ISO
    growth cone GO:0030426 IEA
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 IDA
    SCAR complex GO:0031209 ISO
    cell leading edge GO:0031252 IDA
    filopodium tip GO:0032433 IEA
    filopodium tip GO:0032433 ISO
    neuron projection GO:0043005 IDA
    actin-based cell projection GO:0098858 IBA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Abi1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Abi1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Wasf1  
Co-fractionation Mus musculus
2 Eps8  
Affinity Capture-Western Mus musculus
3 Tnik  
Affinity Capture-MS Mus musculus
4 PRKAA1 5562
Two-hybrid Homo sapiens
5 Cyfip2 76884
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Nckap1l 105855
Affinity Capture-MS Mus musculus
7 Pik3r1  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Far Western Mus musculus
8 Tsc1  
Affinity Capture-MS Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Dlg4  
Affinity Capture-MS Mus musculus
12 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
13 Hras 15461
Phenotypic Suppression Mus musculus
14 Mrfap1  
Co-fractionation Mus musculus
15 Wasf2 242687
Affinity Capture-MS Mus musculus
16 Nckap1 50884
Affinity Capture-MS Mus musculus
17 Tmem173  
Proximity Label-MS Mus musculus
18 Nck2 17974
Two-hybrid Mus musculus
19 Cyfip1 20430
Affinity Capture-MS Mus musculus
20 Brk1  
Affinity Capture-MS Mus musculus
21 Abl2  
Biochemical Activity Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
22 Tcerg1  
Co-fractionation Mus musculus
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