Gene description for Nck2
Gene name non-catalytic region of tyrosine kinase adaptor protein 2
Gene symbol Nck2
Other names/aliases 4833426I10Rik
Grb4
NCKbeta
Nck-2
Species Mus musculus
 Database cross references - Nck2
ExoCarta ExoCarta_17974
Vesiclepedia VP_17974
Entrez Gene 17974
UniProt O55033  
 Nck2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nck2
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    receptor tyrosine kinase binding GO:0030971 IBA
    signaling adaptor activity GO:0035591 IBA
    protein-containing complex binding GO:0044877 IPI
    scaffold protein binding GO:0097110 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    immunological synapse formation GO:0001771 ISO
    actin filament organization GO:0007015 IMP
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IMP
    lamellipodium assembly GO:0030032 IMP
    positive regulation of actin filament polymerization GO:0030838 IEA
    positive regulation of actin filament polymerization GO:0030838 ISO
    response to endoplasmic reticulum stress GO:0034976 IGI
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IBA
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IGI
    positive regulation of T cell proliferation GO:0042102 IEA
    positive regulation of T cell proliferation GO:0042102 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    ephrin receptor signaling pathway GO:0048013 IBA
    ephrin receptor signaling pathway GO:0048013 IDA
    dendritic spine development GO:0060996 ISO
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IBA
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IGI
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IBA
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IGI
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 IGI
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 TAS
    vesicle membrane GO:0012506 IDA
    postsynaptic density GO:0014069 ISO
    synapse GO:0045202 ISO
 Experiment description of studies that identified Nck2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nck2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Arhgap26  
Co-fractionation Mus musculus
2 Prickle2  
Co-fractionation Mus musculus
3 Dnm1 13429
Two-hybrid Mus musculus
4 Syngap1  
Co-fractionation Mus musculus
5 Hnrnpk 15387
Two-hybrid Mus musculus
6 Vav1 22324
Co-fractionation Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Sorbs2  
Co-fractionation Mus musculus
9 Abi1 11308
Two-hybrid Mus musculus
10 Syne2  
Co-fractionation Mus musculus
11 Sorbs1  
Co-fractionation Mus musculus
12 Arhgap21  
Co-fractionation Mus musculus
13 Arhgap42  
Co-fractionation Mus musculus
14 Dok1  
Affinity Capture-MS Mus musculus
15 Axin1  
Two-hybrid Mus musculus
16 Dok2  
Affinity Capture-MS Mus musculus
17 Efnb1  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
18 Arhgap15  
Co-fractionation Mus musculus
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