Gene description for Mthfd1
Gene name methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Gene symbol Mthfd1
Other names/aliases Dcs
E430024A07Rik
Mthfd
Species Mus musculus
 Database cross references - Mthfd1
ExoCarta ExoCarta_108156
Vesiclepedia VP_108156
Entrez Gene 108156
UniProt Q922D8  
 Mthfd1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mthfd1
Molecular Function
    formate-tetrahydrofolate ligase activity GO:0004329 IMP
    formate-tetrahydrofolate ligase activity GO:0004329 ISO
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 IBA
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 ISO
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 ISS
    methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity GO:0004486 ISO
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 IDA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IBA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IMP
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 ISO
    ATP binding GO:0005524 IEA
Biological Process
    L-histidine biosynthetic process GO:0000105 IEA
    neutrophil homeostasis GO:0001780 IMP
    neural tube closure GO:0001843 IMP
    purine nucleotide biosynthetic process GO:0006164 IMP
    purine nucleotide biosynthetic process GO:0006164 ISO
    methionine metabolic process GO:0006555 IMP
    one-carbon metabolic process GO:0006730 ISO
    heart development GO:0007507 IMP
    serine family amino acid metabolic process GO:0009069 IMP
    serine family amino acid biosynthetic process GO:0009070 IEA
    serine family amino acid biosynthetic process GO:0009070 ISO
    methionine biosynthetic process GO:0009086 IEA
    methionine biosynthetic process GO:0009086 ISO
    purine ribonucleotide biosynthetic process GO:0009152 IMP
    10-formyltetrahydrofolate biosynthetic process GO:0009257 IMP
    10-formyltetrahydrofolate biosynthetic process GO:0009257 ISO
    transsulfuration GO:0019346 IMP
    tetrahydrofolate interconversion GO:0035999 IBA
    tetrahydrofolate interconversion GO:0035999 IEA
    tetrahydrofolate interconversion GO:0035999 IMP
    tetrahydrofolate interconversion GO:0035999 ISO
    somite development GO:0061053 IMP
Subcellular Localization
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Mthfd1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mthfd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Rps21 66481
Co-fractionation Mus musculus
4 Nans 94181
Co-fractionation Mus musculus
5 Uba2 50995
Co-fractionation Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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