Gene description for Uba2
Gene name ubiquitin-like modifier activating enzyme 2
Gene symbol Uba2
Other names/aliases AA986091
Arx
Sae2
UBA1
Ubl1a2
Uble1b
Species Mus musculus
 Database cross references - Uba2
ExoCarta ExoCarta_50995
Vesiclepedia VP_50995
Entrez Gene 50995
UniProt Q9Z1F9  
 Uba2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Uba2
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    transcription factor binding GO:0008134 IPI
    transferase activity GO:0016740 IEA
    SUMO activating enzyme activity GO:0019948 IDA
    SUMO activating enzyme activity GO:0019948 ISO
    SUMO activating enzyme activity GO:0019948 ISO
    SUMO activating enzyme activity GO:0019948 ISS
    SUMO binding GO:0032183 IEA
    SUMO binding GO:0032183 ISO
    small protein activating enzyme binding GO:0044388 IEA
    small protein activating enzyme binding GO:0044388 ISO
    ubiquitin-like protein conjugating enzyme binding GO:0044390 IEA
    ubiquitin-like protein conjugating enzyme binding GO:0044390 ISO
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    protein sumoylation GO:0016925 IBA
    protein sumoylation GO:0016925 IEA
    protein sumoylation GO:0016925 ISO
    protein sumoylation GO:0016925 ISS
    positive regulation of protein sumoylation GO:0033235 IEA
    positive regulation of protein sumoylation GO:0033235 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    SUMO activating enzyme complex GO:0031510 IBA
    SUMO activating enzyme complex GO:0031510 IDA
    SUMO activating enzyme complex GO:0031510 ISO
    SUMO activating enzyme complex GO:0031510 ISS
 Experiment description of studies that identified Uba2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Uba2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ube2i  
Co-fractionation Mus musculus
2 Serbp1 66870
Co-fractionation Mus musculus
3 Ireb2  
Co-fractionation Mus musculus
4 Ufd1l  
Co-fractionation Mus musculus
5 USP2  
Biochemical Activity Homo sapiens
6 Eed  
Affinity Capture-MS Mus musculus
7 Zpr1  
Co-fractionation Mus musculus
8 Plaa  
Co-fractionation Mus musculus
9 Mthfd1 108156
Co-fractionation Mus musculus
10 Hnrnpul2 68693
Co-fractionation Mus musculus
11 Sae1 56459
Co-fractionation Mus musculus
12 Rabggta  
Co-fractionation Mus musculus
13 Nploc4  
Co-fractionation Mus musculus
14 Rad23b 19359
Co-fractionation Mus musculus
15 Ssb 20823
Co-fractionation Mus musculus
16 Fancd2  
Affinity Capture-MS Mus musculus
17 Srp14  
Co-fractionation Mus musculus
18 Pde12  
Co-fractionation Mus musculus
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