Gene description for Rcc2
Gene name regulator of chromosome condensation 2
Gene symbol Rcc2
Other names/aliases 2610510H01Rik
2610529N02Rik
AA536646
AA675016
Td60
mKIAA1470
Species Mus musculus
 Database cross references - Rcc2
ExoCarta ExoCarta_108911
Vesiclepedia VP_108911
Entrez Gene 108911
UniProt Q8BK67  
 Rcc2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rcc2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IPI
    small GTPase binding GO:0031267 ISO
Biological Process
    integrin-mediated signaling pathway GO:0007229 IEA
    integrin-mediated signaling pathway GO:0007229 ISO
    regulation of fibroblast migration GO:0010762 IMP
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IEA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 ISO
    regulation of cell migration GO:0030334 IMP
    negative regulation of GTPase activity GO:0034260 IMP
    negative regulation of GTPase activity GO:0034260 ISO
    establishment of protein localization GO:0045184 IGI
    focal adhesion assembly GO:0048041 IEA
    focal adhesion assembly GO:0048041 ISO
    cell division GO:0051301 IEA
    negative regulation of focal adhesion assembly GO:0051895 IMP
    positive regulation of attachment of spindle microtubules to kinetochore GO:0051987 IBA
    positive regulation of attachment of spindle microtubules to kinetochore GO:0051987 IEA
    positive regulation of attachment of spindle microtubules to kinetochore GO:0051987 ISO
    chromosome passenger complex localization to kinetochore GO:0072356 IBA
    chromosome passenger complex localization to kinetochore GO:0072356 IEA
    chromosome passenger complex localization to kinetochore GO:0072356 ISO
    activation of GTPase activity GO:0090630 IGI
    negative regulation of substrate adhesion-dependent cell spreading GO:1900025 IMP
    regulation of ruffle assembly GO:1900027 IGI
    regulation of ruffle assembly GO:1900027 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IEA
    microtubule GO:0005874 IEA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IBA
    membrane GO:0016020 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    early endosome membrane GO:0031901 IDA
    chromosome, centromeric core domain GO:0034506 IEA
    chromosome, centromeric core domain GO:0034506 ISO
    mitotic spindle midzone GO:1990023 IEA
    mitotic spindle midzone GO:1990023 ISO
 Experiment description of studies that identified Rcc2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rcc2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 Hdgf  
Co-fractionation Mus musculus
3 Rac1 19353
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
4 Chaf1a  
Affinity Capture-MS Mus musculus
5 Atg12  
Positive Genetic Mus musculus
6 Nanog  
Affinity Capture-MS Mus musculus
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