Gene description for CYB561D2
Gene name cytochrome b561 family, member D2
Gene symbol CYB561D2
Other names/aliases 101F6
TSP10
XXcos-LUCA11.4
Species Homo sapiens
 Database cross references - CYB561D2
ExoCarta ExoCarta_11068
Vesiclepedia VP_11068
Entrez Gene 11068
HGNC 30253
MIM 607068
UniProt O14569  
 CYB561D2 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CYB561D2
Molecular Function
    protein binding GO:0005515 IPI
    heme binding GO:0020037 IDA
    metal ion binding GO:0046872 IEA
    transmembrane ascorbate ferrireductase activity GO:0140571 ISS
    transmembrane monodehydroascorbate reductase activity GO:0140575 IDA
Biological Process
    transmembrane transport GO:0055085 IEA
    ascorbate homeostasis GO:0140576 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 IEA
    cytoplasmic vesicle membrane GO:0030659 IEA
    vesicle GO:0031982 ISS
 Experiment description of studies that identified CYB561D2 in sEVs
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CYB561D2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 SLC44A1 23446
Affinity Capture-MS Homo sapiens
3 TMEM45B 120224
Two-hybrid Homo sapiens
4 TMEM154  
Two-hybrid Homo sapiens
5 NTHL1  
Affinity Capture-MS Homo sapiens
6 UNC119 9094
Two-hybrid Homo sapiens
7 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
8 FAM209A 200232
Two-hybrid Homo sapiens
9 PTPN5  
Two-hybrid Homo sapiens
10 ACACA 31
Positive Genetic Homo sapiens
11 ERGIC3 51614
Two-hybrid Homo sapiens
12 GPX8 493869
Two-hybrid Homo sapiens
13 ARL13B 200894
Two-hybrid Homo sapiens
14 SLC30A2  
Two-hybrid Homo sapiens
15 CLEC14A  
Two-hybrid Homo sapiens
16 CLDN7 1366
Two-hybrid Homo sapiens
17 PGRMC2 10424
Two-hybrid Homo sapiens
18 CD79A  
Two-hybrid Homo sapiens
19 PSCA 8000
Two-hybrid Homo sapiens
20 RMDN3 55177
Two-hybrid Homo sapiens
21 CREB3L1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CYB561D2 is involved
No pathways found





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