Gene description for AGAP3
Gene name ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
Gene symbol AGAP3
Other names/aliases AGAP-3
CENTG3
CRAG
MRIP-1
cnt-g3
Species Homo sapiens
 Database cross references - AGAP3
ExoCarta ExoCarta_116988
Vesiclepedia VP_116988
Entrez Gene 116988
HGNC 16923
UniProt Q96P47  
 AGAP3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for AGAP3
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activator activity GO:0005096 IBA
    GTP binding GO:0005525 IEA
    polyubiquitin modification-dependent protein binding GO:0031593 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    cellular response to reactive oxygen species GO:0034614 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    postsynaptic density GO:0014069 IEA
    cell periphery GO:0071944 ISO
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified AGAP3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AGAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CLIP1 6249
Proximity Label-MS Homo sapiens
4 MIER2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 AHCYL1 10768
Affinity Capture-MS Homo sapiens
6 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PRNP 5621
Affinity Capture-MS Homo sapiens
8 AGAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 YWHAB 7529
Affinity Capture-MS Homo sapiens
11 RACGAP1 29127
Affinity Capture-MS Homo sapiens
12 RPA2 6118
Proximity Label-MS Homo sapiens
13 FGF12  
Affinity Capture-MS Homo sapiens
14 LAMP1 3916
Proximity Label-MS Homo sapiens
15 NUFIP2 57532
Affinity Capture-MS Homo sapiens
16 RABEP2 79874
Affinity Capture-MS Homo sapiens
17 CD6 923
Affinity Capture-MS Homo sapiens
18 TANC2  
Affinity Capture-MS Homo sapiens
19 NBEA  
Affinity Capture-MS Homo sapiens
20 MCC 4163
Affinity Capture-MS Homo sapiens
21 HSPB1 3315
Two-hybrid Homo sapiens
22 FKBP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HSPA8 3312
Affinity Capture-MS Homo sapiens
24 ARF6 382
Proximity Label-MS Homo sapiens
25 METTL21B  
Affinity Capture-MS Homo sapiens
26 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CALM1 801
Affinity Capture-MS Homo sapiens
28 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
29 FLCN 201163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MCAM 4162
Proximity Label-MS Homo sapiens
31 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SKIDA1  
Affinity Capture-MS Homo sapiens
33 TANC1 85461
Affinity Capture-MS Homo sapiens
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 UBE2O 63893
Affinity Capture-MS Homo sapiens
36 ZCCHC10  
Affinity Capture-MS Homo sapiens
37 DCTN1 1639
Affinity Capture-MS Homo sapiens
38 S100B 6285
Affinity Capture-MS Homo sapiens
39 RPA4  
Proximity Label-MS Homo sapiens
40 TNFRSF1B  
Affinity Capture-MS Homo sapiens
41 C12orf57 113246
Affinity Capture-MS Homo sapiens
42 YWHAG 7532
Affinity Capture-MS Homo sapiens
43 SLC31A1 1317
Affinity Capture-MS Homo sapiens
44 SPANXN2  
Affinity Capture-MS Homo sapiens
45 CETN2 1069
Affinity Capture-MS Homo sapiens
46 TSC22D3  
Affinity Capture-MS Homo sapiens
47 BRD3 8019
Affinity Capture-MS Homo sapiens
48 NCKAP5  
Affinity Capture-MS Homo sapiens
49 PAGE1  
Affinity Capture-MS Homo sapiens
50 HUNK  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AGAP3 is involved
No pathways found





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