Gene description for RACGAP1
Gene name Rac GTPase activating protein 1
Gene symbol RACGAP1
Other names/aliases CYK4
HsCYK-4
ID-GAP
MgcRacGAP
Species Homo sapiens
 Database cross references - RACGAP1
ExoCarta ExoCarta_29127
Vesiclepedia VP_29127
Entrez Gene 29127
HGNC 9804
MIM 604980
UniProt Q9H0H5  
 RACGAP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Prostate cancer cells 25844599    
Saliva 19199708    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for RACGAP1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 IMP
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    microtubule binding GO:0008017 IDA
    protein kinase binding GO:0019901 IPI
    protein-macromolecule adaptor activity GO:0030674 IDA
    alpha-tubulin binding GO:0043014 IDA
    gamma-tubulin binding GO:0043015 IDA
    metal ion binding GO:0046872 IEA
    beta-tubulin binding GO:0048487 IDA
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    mitotic cytokinesis GO:0000281 IC
    mitotic cytokinesis GO:0000281 IDA
    mitotic cytokinesis GO:0000281 IMP
    actomyosin contractile ring assembly GO:0000915 IMP
    monoatomic ion transport GO:0006811 IEA
    Rho protein signal transduction GO:0007266 IBA
    spermatogenesis GO:0007283 IEP
    neuroblast proliferation GO:0007405 ISS
    erythrocyte differentiation GO:0030218 IMP
    positive regulation of cytokinesis GO:0032467 IDA
    positive regulation of cytokinesis GO:0032467 IMP
    regulation of embryonic development GO:0045995 ISS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    mitotic spindle midzone assembly GO:0051256 IBA
    mitotic spindle midzone assembly GO:0051256 IDA
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 IMP
    sulfate transmembrane transport GO:1902358 IDA
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    spindle GO:0005819 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    midbody GO:0030496 IBA
    midbody GO:0030496 IDA
    cleavage furrow GO:0032154 IBA
    cleavage furrow GO:0032154 IDA
    spindle midzone GO:0051233 IBA
    spindle midzone GO:0051233 IDA
    extracellular exosome GO:0070062 HDA
    mitotic spindle GO:0072686 IDA
    mitotic spindle GO:0072686 IMP
    Flemming body GO:0090543 IEA
    centralspindlin complex GO:0097149 IBA
    centralspindlin complex GO:0097149 IDA
 Experiment description of studies that identified RACGAP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
24
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
25
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RACGAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-MS Homo sapiens
2 NET1 10276
Affinity Capture-MS Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 DPYSL2 1808
Affinity Capture-MS Homo sapiens
6 PPP2R4 5524
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 FAM98A 25940
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ARID5B 84159
Affinity Capture-MS Homo sapiens
9 TCTN3  
Affinity Capture-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
11 Calml3  
Affinity Capture-MS Mus musculus
12 Chmp4b 75608
Affinity Capture-MS Mus musculus
13 CD2AP 23607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TNFRSF8 943
Affinity Capture-MS Homo sapiens
15 TAB1 10454
Affinity Capture-MS Homo sapiens
16 ROCK2 9475
Co-fractionation Homo sapiens
17 TMEM263 90488
Affinity Capture-MS Homo sapiens
18 COPA 1314
Affinity Capture-MS Homo sapiens
19 AGAP3 116988
Affinity Capture-MS Homo sapiens
20 CENPA  
Proximity Label-MS Homo sapiens
21 APEX1 328
Proximity Label-MS Homo sapiens
22 CAPZB 832
Affinity Capture-MS Homo sapiens
23 SAMHD1 25939
Affinity Capture-MS Homo sapiens
24 PCNT  
Affinity Capture-MS Homo sapiens
25 BCL7B  
Affinity Capture-MS Homo sapiens
26 DYRK1A 1859
Affinity Capture-MS Homo sapiens
27 KIF23 9493
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 Cep55 74107
Affinity Capture-MS Mus musculus
29 FZR1  
Affinity Capture-Western Homo sapiens
30 PRC1 9055
Affinity Capture-MS Homo sapiens
31 ERC1 23085
Affinity Capture-MS Homo sapiens
32 CALM3 808
Affinity Capture-MS Homo sapiens
33 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 STX18 53407
Affinity Capture-MS Homo sapiens
35 HMGA1 3159
Affinity Capture-MS Homo sapiens
36 CSTF1 1477
Affinity Capture-MS Homo sapiens
37 RSRC1  
Affinity Capture-MS Homo sapiens
38 Kif23 71819
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
39 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ELMO2 63916
Affinity Capture-MS Homo sapiens
41 SFN 2810
Affinity Capture-MS Homo sapiens
42 CUL1 8454
Affinity Capture-MS Homo sapiens
43 HSD17B4 3295
Affinity Capture-MS Homo sapiens
44 TRAPPC11 60684
Affinity Capture-MS Homo sapiens
45 WDR5 11091
Affinity Capture-Western Homo sapiens
46 Racgap1 26934
Affinity Capture-MS Mus musculus
47 KIF14 9928
Affinity Capture-MS Homo sapiens
48 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 DAPK2  
Affinity Capture-MS Homo sapiens
50 KLF16  
Proximity Label-MS Homo sapiens
51 CHMP4C 92421
Affinity Capture-MS Homo sapiens
52 PHLDB2 90102
Affinity Capture-MS Homo sapiens
53 VAV1 7409
Reconstituted Complex Homo sapiens
54 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
55 KIF20A 10112
Affinity Capture-MS Homo sapiens
56 PARK2  
Affinity Capture-MS Homo sapiens
57 LDHD  
Affinity Capture-MS Homo sapiens
58 LYN 4067
Proximity Label-MS Homo sapiens
59 RCC2 55920
Affinity Capture-MS Homo sapiens
60 VRK1 7443
Co-fractionation Homo sapiens
61 GID8 54994
Affinity Capture-MS Homo sapiens
62 CHMP4B 128866
Affinity Capture-MS Homo sapiens
63 SIPA1L2  
Affinity Capture-MS Homo sapiens
64 TANK 10010
Two-hybrid Homo sapiens
65 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
66 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
68 ANAPC4 29945
Affinity Capture-MS Homo sapiens
69 HECTD1 25831
Affinity Capture-MS Homo sapiens
70 SHCBP1 79801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 EOGT 285203
Affinity Capture-MS Homo sapiens
72 KLF12  
Proximity Label-MS Homo sapiens
73 SYBU  
Affinity Capture-MS Homo sapiens
74 TXNDC11 51061
Two-hybrid Homo sapiens
75 ANLN 54443
Affinity Capture-MS Homo sapiens
76 ACTL8  
Affinity Capture-MS Homo sapiens
77 STON2 85439
Affinity Capture-MS Homo sapiens
78 SLC26A8  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
79 HIST1H3A 8350
Proximity Label-MS Homo sapiens
80 Cd2ap 12488
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
81 C9orf78 51759
Affinity Capture-MS Homo sapiens
82 EGLN3  
Affinity Capture-MS Homo sapiens
83 MRPL35  
Affinity Capture-MS Homo sapiens
84 ECT2 1894
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
85 YWHAZ 7534
Affinity Capture-MS Homo sapiens
86 RASIP1  
Affinity Capture-MS Homo sapiens
87 EGFR 1956
Negative Genetic Homo sapiens
88 CHEK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 ARF6 382
Proximity Label-MS Homo sapiens
90 MORF4L1  
Two-hybrid Homo sapiens
91 PSMD7 5713
Affinity Capture-MS Homo sapiens
92 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
93 RFC1 5981
Co-fractionation Homo sapiens
94 AURKB 9212
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
95 ZYX 7791
Affinity Capture-MS Homo sapiens
96 BAZ2A  
Affinity Capture-MS Homo sapiens
97 PCBP1 5093
Affinity Capture-MS Homo sapiens
98 RND2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
99 RAPGEF2 9693
Affinity Capture-MS Homo sapiens
100 RPA3 6119
Proximity Label-MS Homo sapiens
101 Tpx2  
Affinity Capture-MS Mus musculus
102 PKP4 8502
Affinity Capture-MS Homo sapiens
103 TRIM27  
Affinity Capture-MS Homo sapiens
104 AKT2 208
Affinity Capture-MS Homo sapiens
105 MOB2 81532
Affinity Capture-MS Homo sapiens
106 CUL7 9820
Affinity Capture-MS Homo sapiens
107 DDRGK1 65992
Affinity Capture-MS Homo sapiens
108 RHOB 388
Proximity Label-MS Homo sapiens
109 MPRIP 23164
Two-hybrid Homo sapiens
110 PIN4 5303
Affinity Capture-MS Homo sapiens
111 HIF1A 3091
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
112 SURF6  
Affinity Capture-MS Homo sapiens
113 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
114 ANKFY1 51479
Affinity Capture-MS Homo sapiens
115 COPZ1 22818
Affinity Capture-MS Homo sapiens
116 PARP1 142
Proximity Label-MS Homo sapiens
117 LIG3 3980
Co-fractionation Homo sapiens
118 GRB2 2885
Affinity Capture-MS Homo sapiens
119 DLG3 1741
Affinity Capture-MS Homo sapiens
120 KLHL8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 EIF4G1 1981
Affinity Capture-MS Homo sapiens
122 Zw10  
Affinity Capture-MS Mus musculus
123 BYSL 705
Two-hybrid Homo sapiens
124 IKBKG 8517
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
125 SMNDC1  
Co-fractionation Homo sapiens
126 CAPZA2 830
Affinity Capture-MS Homo sapiens
127 CHD6 84181
Affinity Capture-MS Homo sapiens
128 CSTB 1476
Affinity Capture-MS Homo sapiens
129 RABL6 55684
Affinity Capture-MS Homo sapiens
130 IPP  
Affinity Capture-MS Homo sapiens
131 MKI67  
Affinity Capture-MS Homo sapiens
132 CDK1 983
Biochemical Activity Homo sapiens
133 SP6  
Affinity Capture-MS Homo sapiens
134 MED21  
Affinity Capture-MS Homo sapiens
135 E2F4  
Affinity Capture-MS Homo sapiens
136 CDK3 1018
Two-hybrid Homo sapiens
137 MICAL3 57553
Affinity Capture-MS Homo sapiens
138 ENTPD6  
Affinity Capture-MS Homo sapiens
139 ANKRD11 29123
Affinity Capture-MS Homo sapiens
140 C14orf166 51637
Affinity Capture-MS Homo sapiens
141 Flot1 14251
Affinity Capture-MS Mus musculus
142 C2CD5 9847
Affinity Capture-MS Homo sapiens
143 SNW1 22938
Affinity Capture-MS Homo sapiens
144 KRAS 3845
Negative Genetic Homo sapiens
145 Shcbp1 20419
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
146 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
147 PPP1CB 5500
Affinity Capture-MS Homo sapiens
148 RAC1 5879
FRET Homo sapiens
149 PSMD1 5707
Co-fractionation Homo sapiens
150 PRSS23 11098
Two-hybrid Homo sapiens
151 CDC5L 988
Affinity Capture-MS Homo sapiens
152 PLG 5340
Affinity Capture-MS Homo sapiens
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